Differential immunoreactivity of goat derived scrapie following in vitro misfolding versus mouse bioassay.

abstract：:This study investigated the effects of shear stress on gene expression of prostacyclin synthesis-related enzymes cyclooxygenases (COX-1 and COX-2), prostacyclin synthase (PGS), and thromboxane synthase (TXS) and their metabolites prostaglandin (PGI(2)) and thromboxane A(2) (TXA(2)) in endothelium of intact conduit ves...

journal_title：Biochemical and biophysical research communications

authors： Doroudi R,Gan LM,Selin Sjögren L,Jern S

更新日期：2000-03-05 00:00:00

abstract：:The chaperonin containing t-complex polypeptide 1 (CCT) is a heterooligomeric molecular chaperone that assists in the folding of actin, tubulin, and other cytosolic proteins. We show here that degradation of CCT in mammalian cells is inhibited by a proteasome-specific inhibitor, lactacystin. When CCT synthesis was inh...

journal_title：Biochemical and biophysical research communications

authors： Yokota S,Kayano T,Ohta T,Kurimoto M,Yanagi H,Yura T,Kubota H

更新日期：2000-12-20 00:00:00

abstract：:The human histiocyte cell line, U937, with monocyte characteristics, can be induced to differentiate into macrophage-like cells when exposed to growth medium containing 1.5% DMSO. Following three days of exposure, DMSO-treated but not control U937 cells can be stimulated to release endogenous arachidonic acid from the...

journal_title：Biochemical and biophysical research communications

authors： Myers RF,Siegel MI

更新日期：1984-01-13 00:00:00

abstract：:Strategies that antagonize growth factor signaling are attractive candidates for the biological therapy of brain tumors. HGF/NK2 is a secreted truncated splicing variant and potential antagonist of scatter factor/hepatocyte growth factor (SF/HGF), a multifunctional cytokine involved in the malignant progression of sol...

journal_title：Biochemical and biophysical research communications

authors： Guerin C,Luddy C,Abounader R,Lal B,Laterra J

更新日期：2000-06-24 00:00:00

abstract：:Rearrangement of the p53 gene is frequent in virus transformed cell lines and in chronic myelogenous leukemia. It is a rare event in solid tumours and has been reported only in osteosarcomas. In this study we have examined rearrangement of the p53 gene in human breast tumours. We found rearrangement in 35% of the pati...

journal_title：Biochemical and biophysical research communications

authors： Nayak BK,Patnaik S,Das BR

更新日期：1998-04-17 00:00:00

abstract：:Collagen synthesis in human fibroblasts was increased by the presence of 0.1M excess NaCl in the culture medium. Type analysis of the collagen produced by the cells showed the increase in the ratio of type III collagen to the total collagen. Similar NaCl effects were observed in several cell lines derived from differe...

journal_title：Biochemical and biophysical research communications

authors： Hata R,Sunada H,Nagai Y

更新日期：1983-11-30 00:00:00

abstract：:Rheumatoid arthritis (RA) is an autoimmune inflammatory disease exhibited most commonly in joints. We found that the expression of C1qtnf3, which encodes C1q/TNF-related protein 3 (CTRP3), was highly increased in two mouse RA models with different etiology. To elucidate the pathogenic roles of CTRP3 in the development...

journal_title：Biochemical and biophysical research communications

authors： Murayama MA,Kakuta S,Maruhashi T,Shimizu K,Seno A,Kubo S,Sato N,Saijo S,Hattori M,Iwakura Y

更新日期：2014-01-03 00:00:00

abstract：:Separation of proteins by two-dimensional electrophoresis and following mass spectrometry (MS) is now a conventional technique for proteomic analysis. For proteomic analysis of a certain tissue with a limited information of primary structures of proteins, we have developed an analytical system for peptide mass fingerp...

journal_title：Biochemical and biophysical research communications

authors： Hozumi A,Satouh Y,Ishibe D,Kaizu M,Konno A,Ushimaru Y,Toda T,Inaba K

更新日期：2004-07-09 00:00:00

abstract：:The octaheme cytochrome c3 (Mr 26000) from Desulfovibrio desulfuricans Norway was studied using cyclic voltammetry at the pyrolytic graphite electrode. The kinetics of reduction of the octaheme cytochrome c3 (Mr 26000) from D. desulfuricans Norway by the Ni-Fe-Se hydrogenase purified from the same organism was investi...

journal_title：Biochemical and biophysical research communications

authors： Haladjian J,Bianco P,Guerlesquin F,Bruschi M

更新日期：1991-08-30 00:00:00

abstract：:The cDNA encoding a swelling-induced chloride conductance regulatory protein, plcln, was cloned from rabbit ciliary epithelium by using a polymerase chain reaction (PCR)-based approach. The open reading frame encoding 236 amino acids possesses high amino acid identity (93/%) with the previously cloned plcln from human...

journal_title：Biochemical and biophysical research communications

authors： Wan XL,Chen S,Sears M

更新日期：1997-10-29 00:00:00

abstract：:X-ray microanalysis has been used to determine elemental content in the cytoplasm of hepatocytes of hyperthyroid rats in comparison with euthyroid controls. No significant differences were found for any examined element (sodium, phosphorus, sulfur, chlorine, potassium, magnesium and calcium). In contrast to earlier re...

journal_title：Biochemical and biophysical research communications

authors： Smith NK,Lewiński A,Stabler-Morris S

更新日期：1983-08-30 00:00:00

abstract：:We have previously observed that extracellular Mg2+ influences the phosphofructokinase (PFK) activity of intact Ehrlich Ascites tumour cells (EATC). In this study we have investigated the mechanism by which Mg2+ modulates this key glycolytic enzyme in EATC made permeable to the cation by either digitonin or dextran su...

journal_title：Biochemical and biophysical research communications

authors： Wolf FI,Bossi D,Cittadini A

更新日期：1991-09-16 00:00:00

abstract：:Sarafotoxins (SRTXs) are endothelin-like peptides extracted from snake venom. SRTXs stimulate the endothelin ETA and ETB receptors and enhance vasoconstriction, followed by left ventricular dysfunction and bronchoconstriction. SRTXs include four major isopeptides, S6a-d, with different subtype selectivities. Here, we ...

journal_title：Biochemical and biophysical research communications

authors： Izume T,Miyauchi H,Shihoya W,Nureki O

更新日期：2020-07-23 00:00:00

abstract：:Because of the growing importance of the Retinoid X Receptors (RXR alpha, beta and gamma) in the retinoid acid signalling pathway, we have prepared polyclonal and monoclonal antibodies directed against these proteins. For this purpose, either the whole mouse RXR alpha protein expressed in E.Coli, or synthetic peptides...

journal_title：Biochemical and biophysical research communications

authors： Rochette-Egly C,Lutz Y,Pfister V,Heyberger S,Scheuer I,Chambon P,Gaub MP

更新日期：1994-10-28 00:00:00

abstract：:Adiponectin is an adipocyte-derived hormone and known to form several species of multimer, however, the precise components of each multimer have not been fully determined. We purified each multimer adiponectin selectively from human plasma and characterized them by affinity columns using anti-adiponectin, gelatin, or ...

journal_title：Biochemical and biophysical research communications

authors： Hada Y,Yamauchi T,Waki H,Tsuchida A,Hara K,Yago H,Miyazaki O,Ebinuma H,Kadowaki T

更新日期：2007-05-04 00:00:00

abstract：:Activins, TGF-beta superfamily members, have multiple functions in a variety of cells and tissues. Recently, additional activin beta subunit genes, betaC and betaE, have been identified. To explore the role of activin E, we created transgenic mice overexpressing human activin betaE subunit. There were pronounced diffe...

journal_title：Biochemical and biophysical research communications

authors： Hashimoto O,Ushiro Y,Sekiyama K,Yamaguchi O,Yoshioka K,Mutoh K,Hasegawa Y

更新日期：2006-03-10 00:00:00

abstract：:Atrial- and brain-type natriuretic peptides (ANP and BNP, respectively) have been shown to exert potent lipolytic action in adipocytes. A family of natriuretic peptide receptors (NPRs), NPR-1, NPR-2, and NPR-3, mediates their physiologic effects. NPR-1 and NPR-2 are receptor guanylyl cyclases, while NPR-3 lacks enzyma...

journal_title：Biochemical and biophysical research communications

authors： Nakatsuji H,Maeda N,Hibuse T,Hiuge A,Hirata A,Kuroda Y,Kishida K,Kihara S,Funahashi T,Shimomura I

更新日期：2010-01-29 00:00:00

abstract：:The molecular structures of enzyme mimics may be modified to optimize their catalytic properties. In this study, to generate artificial enzyme mimics, Watson-Crick base paired DNA duplexes were designed as scaffolds which were assembled by nucleotides modified with specific functional groups. This process allowed vari...

journal_title：Biochemical and biophysical research communications

authors： Xu L,Ji C,Bai Y,He J,Liu K

更新日期：2013-05-10 00:00:00

abstract：:Bovine erythrocyte green heme binding protein and bovine erythrocyte flavin reductase have been isolated in highly purified forms and subjected to amino acid analysis and N-terminal amino acid sequence analysis. The two proteins possess similar amino acid compositions and identical N-terminal amino acid sequences. Mor...

journal_title：Biochemical and biophysical research communications

authors： Quandt KS,Xu F,Chen P,Hultquist DE

更新日期：1991-07-15 00:00:00

abstract：:Cathepsin D, a lysosomal aspartic proteinase, has been shown to induce apoptosis of HeLa cells when overexpressed. To further understand regulatory mechanisms of cathepsin D-induced cell death, we examined whether lysosomal cysteine and aspartic proteinases are involved in apoptosis of PC12 cells following serum depri...

journal_title：Biochemical and biophysical research communications

authors： Shibata M,Kanamori S,Isahara K,Ohsawa Y,Konishi A,Kametaka S,Watanabe T,Ebisu S,Ishido K,Kominami E,Uchiyama Y

更新日期：1998-10-09 00:00:00

abstract：:We report the construction of a cDNA clone encoding a functional GM2-activator protein. The sequence of the complete 5' end of the coding region was determined by direct nucleotide sequencing of a fragment generated by multiple RACE PCR procedures from Hela cell cDNA. Specific oligonucleotides were synthesized from th...

journal_title：Biochemical and biophysical research communications

authors： Xie B,McInnes B,Neote K,Lamhonwah AM,Mahuran D

更新日期：1991-06-28 00:00:00

abstract：:To examine the possibility that the Orf135 protein of Escherichia coli functions as a hydrolyzing enzyme for a damaged DNA precursor (deoxyribonucleoside 5'-triphosphate), we purified the recombinant Orf135 protein and incubated it with oxidized deoxynucleotides. Of the nucleotides tested, 2-hydroxydeoxyadenosine 5'-t...

journal_title：Biochemical and biophysical research communications

authors： Kamiya H,Murata-Kamiya N,Iida E,Harashima H

更新日期：2001-11-02 00:00:00

abstract：:High ethanol intake is considered to impair insulin sensitivity. In the present study, we investigated the acute and chronic effects of ethanol intake on glucose metabolism and insulin signal transduction. Hyperinsulinemic-euglycemic clamp studies revealed 70% and 51% decreases in the glucose infusion rate, 52% and 31...

journal_title：Biochemical and biophysical research communications

authors： Onishi Y,Honda M,Ogihara T,Sakoda H,Anai M,Fujishiro M,Ono H,Shojima N,Fukushima Y,Inukai K,Katagiri H,Kikuchi M,Oka Y,Asano T

更新日期：2003-04-11 00:00:00

abstract：:Four conserved residues of Clostridium thermocellum endoglucanase CelC were replaced by site-directed mutagenesis. Proteins mutated in His-90, Asn-139 and Glu-140 showed strongly reduced activity, in agreement with predictions of sequence alignments. Mutations in Glu-140 did not result in any detectable change in Km, ...

journal_title：Biochemical and biophysical research communications

authors： Navas J,Béguin P

更新日期：1992-12-15 00:00:00

abstract：:Previously we found a depression in the activity of hepatic xanthine dehydrogenase in rats fed clofibrate or di(2-ethylhexyl)phthalate. The present study further demonstrated that dietary addition of other peroxisome proliferators, including bezafibrate, dehydroepiandrosterone and perfluoro-n-octanoic acid also depres...

journal_title：Biochemical and biophysical research communications

authors： Satoh A,Sasago S,Takahashi S,Kato N

更新日期：1993-09-15 00:00:00

abstract：:Human adipose-derived mesenchymal stem cells (hASCs) are attractive cell source for skin tissue engineering. The aim of this study was to investigate the effects of low-level light therapy (LLLT) on transplanted cluster hASC in a skin wound animal model. The hASCs were cultured in monolayer or clusters. The LLLT, hASC...

journal_title：Biochemical and biophysical research communications

authors： Park IS,Chung PS,Ahn JC

更新日期：2015-07-03 00:00:00

abstract：:Breast cancer amplified sequence 2 (BCAS2) was initially identified as a gene that was overexpressed and amplified in some breast cancer cell lines. It was later found to be a component of the spliceosome. Here, we identified BCAS2 as an estrogen receptor (ER) alpha interacting protein by yeast two-hybrid screening. I...

journal_title：Biochemical and biophysical research communications

authors： Qi C,Zhu YT,Chang J,Yeldandi AV,Rao MS,Zhu YJ

更新日期：2005-03-11 00:00:00

abstract：:Metabolic deregulation accompanying type II diabetes is characterized by insulin resistance in peripheral tissues (liver, muscle, and adipose), mediated by impairments in insulin receptor (IR) signaling. Protein tyrosine phosphatase 1B (PTP1B) has been shown to be a negative regulator of IR autophosphorylation and thu...

journal_title：Biochemical and biophysical research communications

authors： Xu J,Li L,Qian Z,Hong J,Shen S,Huang W

更新日期：2005-04-08 00:00:00

abstract：:Monoclonal antibody BB1 was previously shown to recognize the CD80 protein as well as a putative B7.3 molecule, both ligands of the important T-cell receptors CD28 and CTLA-4. We report that BB1 coprecipitated from detergent lysates of human B- and pre-B-cell lines a protein kinase activity, as detected by solid phase...

journal_title：Biochemical and biophysical research communications

authors： Angelisová P,Cerný J,Horejsí V

更新日期：1996-11-12 00:00:00

abstract：:Several lines of evidence suggest that TNT detoxification by Phanerochaete chrysosporium is through reduction. Rates of TNT reduction were directly correlated with mycelial mass and TNT concentration. Toxicity was inversely related to the amount of fungus. TNT toxicity was identical in both ligninolytic and nonlignino...

journal_title：Biochemical and biophysical research communications

authors： Stahl JD,Aust SD

更新日期：1993-04-30 00:00:00