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Local database and the search program for proteomic analysis of sperm proteins in the ascidian Ciona intestinalis.

Abstract：

:Separation of proteins by two-dimensional electrophoresis and following mass spectrometry (MS) is now a conventional technique for proteomic analysis. For proteomic analysis of a certain tissue with a limited information of primary structures of proteins, we have developed an analytical system for peptide mass fingerprinting in gene products in the testis of the ascidian Ciona intestinalis. Ciona sperm proteins were separated by two-dimensional gel electrophoresis and the tryptic fragments were subjected to MALDI-TOF/MS. The mass pattern was searched against on-line databases but resulted in less identification of these proteins. We have constructed a MS database from Ciona testis ESTs and the genome draft sequence, along with a newly devised, perl-based search program PerMS for peptide mass fingerprinting. This system could identify more than 80% of Ciona sperm proteins, suggesting that it could be widely applied for proteomic analysis for a limited tissue with less genomic information.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Hozumi A,Satouh Y,Ishibe D,Kaizu M,Konno A,Ushimaru Y,Toda T,Inaba K

doi

10.1016/j.bbrc.2004.05.118

subject

Has Abstract

pub_date

2004-07-09 00:00:00

pages

1241-6

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006291X04011283

journal_volume

319

pub_type

杂志文章

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