Icariin stimulates angiogenesis by activating the MEK/ERK- and PI3K/Akt/eNOS-dependent signal pathways in human endothelial cells.

abstract：:We reported that ganglioside GD3 enhances cell proliferation and invasion of melanomas causing stronger tyrosine-phosphorylation of p130Cas and paxillin after stimulation with fetal calf serum. Besides signals via growth factor/receptor, adhesion signals via integrin might be also enhanced by GD3. Here, roles of integ...

journal_title：Biochemical and biophysical research communications

authors： Ohkawa Y,Miyazaki S,Miyata M,Hamamura K,Furukawa K,Furukawa K

更新日期：2008-08-15 00:00:00

abstract：:The production of recombinant hepatitis B virus surface antigen (rHBsAg) purified by immunoaffinity chromatography with monoclonal antibodies is used to obtain a vaccine against this virus. Monoclonal antibodies to rHBsAg from mouse ascites have been purified by Staphylococcal Protein A (SpA)--prior coupling to Sephar...

journal_title：Biochemical and biophysical research communications

authors： Valdés Veliz R,García J,Reyes B,Muñoz L,Alvarez T,Padilla S,Abrahantes Mdel C,Zubiaurrez J,Agraz A,Marrero A

更新日期：2003-04-11 00:00:00

abstract：:It has been demonstrated that joint cultivation of human K-562 cells and mouse L-929 cells results in triggering apoptosis in L-929 cells and possibly also in K-562 cells. Analysis of proteins recovered from the culture medium has revealed the presence of three cytotoxic factors including TNF-alpha. This is the first ...

journal_title：Biochemical and biophysical research communications

authors： Gnuchev NV,Sashchenko LP,Iarovaia OV,Dukhanina E,Lukianova T,Razin SV

更新日期：1997-05-29 00:00:00

abstract：:Up to 1 mol of phosphoryl groups was incorporated per mol of eukaryotic protein synthesis initiation factor (eIF) 4E following incubation of purified preparations of this factor with purified preparations of a protamine kinase from bovine kidney cytosol. By contrast, purified preparations of two forms of mitogen-activ...

journal_title：Biochemical and biophysical research communications

authors： Amick GD,Damuni Z

更新日期：1992-03-16 00:00:00

abstract：:In isolated rabbit proximal tubules the addition of 2.0 microM but not 0.2 microM ionomycin induced a sustained increase in cell pH ([pH]i). This [pH]i response to 2.0 microM ionomycin was shown to be independent of several transporters such as Na+/H+ exchanger, Na(+)-HCO3- cotransporter, Cl-/HCO3- exchanger, or H(+)-...

journal_title：Biochemical and biophysical research communications

authors： Yamada H,Seki G,Taniguchi S,Uwatoko S,Suzuki K,Kurokawa K

更新日期：1996-08-05 00:00:00

abstract：:microRNAs have been demonstrated to play important roles in cancer development and progression. Hence, identifying functional microRNAs and better understanding of the underlying molecular mechanisms would provide new clues for the development of targeted cancer therapies. Herein, we reported that a microRNA, miR-421 ...

journal_title：Biochemical and biophysical research communications

authors： Chen L,Tang Y,Wang J,Yan Z,Xu R

更新日期：2013-06-14 00:00:00

abstract：:Expression of vascular endothelial growth factor (VEGF) by cultured vascular smooth muscle cells was analyzed. Serum and hypoxia had nearly additive actions on VEGF mRNA expression. The function of the VEGF promoter in smooth muscle cells was analyzed using transient luciferase reporter assays. Serum and hypoxia stimu...

journal_title：Biochemical and biophysical research communications

authors： D'Angelo G,Ladoux A,Frelin C

更新日期：2000-01-07 00:00:00

abstract：:Localization of myoplasmic free calcium was measured in fura2-loaded single rat myocardial cells to determine whether the mechanism of norepinephrine desensitization includes redistribution of calcium. Fluorescence intensities at each pixel were quantitated by use of a photon-counting, microchannel plate camera. From ...

journal_title：Biochemical and biophysical research communications

authors： Erne P,Hermsmeyer K

更新日期：1988-02-29 00:00:00

abstract：:Adiponectin has gained significant attention as a mediator of insulin sensitivity. Recently, two receptors of this adipocyte-secreted hormone, adiponectin receptor 1 (AdipoR1) and 2 (AdipoR2), have been cloned. To improve our understanding of the regulation of these receptors in adipocytes, AdipoR1 and AdipoR2 mRNA wa...

journal_title：Biochemical and biophysical research communications

authors： Blüher M,Fasshauer M,Kralisch S,Schön MR,Krohn K,Paschke R

更新日期：2005-04-15 00:00:00

abstract：:PHF8 is a JmjC domain-containing protein and erases repressive histone marks including H4K20me1 and H3K9me1/2. It binds to H3K4me3, an active histone mark usually located at transcription start sites (TSSs), through its plant homeo-domain, and is thus recruited and enriched in gene promoters. PHF8 is involved in the d...

journal_title：Biochemical and biophysical research communications

authors： Shen Y,Pan X,Zhao H

更新日期：2014-08-15 00:00:00

abstract：:Zinc-finger antiviral protein (ZAP) is a recently isolated host antiviral factor that inhibits the replication of many viruses such as Moloney murine leukemia virus (MLV) and Sindbis virus (SIN) by preventing the accumulation of viral mRNA in the cytoplasm. ZAP comprises four CCCH zinc-finger motifs, the second and fo...

journal_title：Biochemical and biophysical research communications

authors： Jeong MS,Kim EJ,Jang SB

更新日期：2010-06-04 00:00:00

abstract：:First messengers and viral transfection are the two most common ways to stimulate cells for signal output, although their applications are limited. We investigated mechanisms of inducing neural stem cell differentiation using recombinant ephrin-B2/Fc and found that it acted as a ligand and inhibited endogenous ephrin-...

journal_title：Biochemical and biophysical research communications

authors： Xiaodong H,Zhen H,Min S,Zhiming C,Hongyan J,Chong Z,Xuefeng T,Guohua J

更新日期：2013-10-18 00:00:00

abstract：:Tropomyosins are a family of actin binding proteins encoded by a group of highly conserved genes. Humans have four tropomyosin-encoding genes: TPM1, TPM2, TPM3, and TPM4, each of which is known to generate multiple isoforms by alternative splicing, promoters, and 3' end processing. TPM1 is the most versatile and encod...

journal_title：Biochemical and biophysical research communications

authors： Denz CR,Narshi A,Zajdel RW,Dube DK

更新日期：2004-08-06 00:00:00

abstract：:To investigate the role of secondary structure in the substrate specificity of human 72 kDa type IV collagenase, we synthesised linear and cyclic collagen sequence analogs. As Ca2+ plays a crucial role in the enzyme activity, the CD and FTIR spectra of the peptides were also measured in the presence of Ca2+. Most of t...

journal_title：Biochemical and biophysical research communications

authors： Likó Z,Botyánszki J,Bódi J,Vass E,Majer Z,Hollósi M,Süli-Vargha H

更新日期：1996-10-14 00:00:00

abstract：:The poly(rC)-binding protein PCBP2 has multiple functions in post-transcriptional control of host and viral gene expression. Since it interacts with picornaviral RNA structures, it was proposed that PCBP2 regulates viral genome translation and replication. The hepatitis A virus (HAV), an atypical picornavirus, contain...

journal_title：Biochemical and biophysical research communications

authors： Zhang B,Seitz S,Kusov Y,Zell R,Gauss-Müller V

更新日期：2007-12-28 00:00:00

abstract：:The effect of extracellular ATP on intracellular free Ca2+ was characterized in quin2-loaded parotid acinar cells. ATP specifically increased the intracellular Ca2+ concentration six-fold above a basal level of 180 nM. Of other purine nucleotides tested, only adenylylthiodiphosphate (ATP gamma S) had significant activ...

journal_title：Biochemical and biophysical research communications

authors： McMillian MK,Soltoff SP,Cantley LC,Talamo BR

更新日期：1987-12-16 00:00:00

abstract：:We compared the nitric oxide (NO)-generating behavior of nitroglycerin (NTG), pentaerythritol trinitrate (PEtriN) and isosorbide dinitrate (ISDN), in the microsomal preparation of bovine coronary artery smooth muscle cells. The comparative NO generating activities among these nitrates were consistent with their relati...

journal_title：Biochemical and biophysical research communications

authors： Chung SJ,Fung HL

更新日期：1992-06-30 00:00:00

abstract：:We investigated the role of tyrosine phosphorylation of FAK in the stretch-induced MAPKs (extracellular signal-regulated kinase (ERK), p38MAPK) activation in mutant FAK-transfected fibroblasts. In response to uniaxial cyclic stretch (1 Hz, 120% in length), the levels of tyrosine phosphorylation of the Tyr-397 and Tyr-...

journal_title：Biochemical and biophysical research communications

authors： Wang JG,Miyazu M,Matsushita E,Sokabe M,Naruse K

更新日期：2001-10-26 00:00:00

abstract：:The exon/intron organization and the structure of the 5' flanking region of the human thyrotropin receptor gene (hTSH-R) were determined. The hTSH-R gene spans more than 60 kb and is split into ten exons. The extracellular domain is encoded by the first nine exons and part of the last exon, whereas the transmembrane a...

journal_title：Biochemical and biophysical research communications

authors： Gross B,Misrahi M,Sar S,Milgrom E

更新日期：1991-06-14 00:00:00

abstract：:Cellular defects which prevent apoptotic cell death can result in the generation of hyperproliferative disorders and can prevent the effective treatment of such diseases. The majority of cellular defects which result in apoptosis resistance lie upstream of caspase activation. We have described chimeric caspase molecul...

journal_title：Biochemical and biophysical research communications

authors： Shearwin-Whyatt L,Baliga B,Doumanis J,Kumar S

更新日期：2001-04-20 00:00:00

abstract：:A truncated form of 24kDa FGF-2 consisting of 86 NH(2)-terminal amino acids (ATE+31) inhibits cell migration in vitro and tumor development and angiogenesis in vivo. Focal adhesion kinase (FAK) is phosphorylated on tyrosine and serine sites after cell stimulation by growth factors. This study examined the effect of AT...

journal_title：Biochemical and biophysical research communications

authors： Lin AH,Eliceiri BP,Levin EG

更新日期：2009-05-08 00:00:00

abstract：:Previous studies have shown that a specific inhibitor of insulin-like growth factor (IGF) action in vitro can be isolated from normal human serum and subsequently partially purified on an IGF-affinity column. The ability of the inhibitor to bind the IGFs has now been confirmed directly using covalent cross-linking tec...

journal_title：Biochemical and biophysical research communications

authors： Ooi GT,Herington AC

更新日期：1986-05-29 00:00:00

abstract：:Alzheimer's disease (AD) pathology is characterized by plaques, tangles, and neuronal cell loss. The main constituent of plaques is beta-amyloid peptide (A beta), a 39-42 residue peptide which has been linked to disruption of calcium homeostasis and neurotoxicity in vitro. We demonstrate that a neurotoxic fragment of ...

journal_title：Biochemical and biophysical research communications

authors： Mirzabekov T,Lin MC,Yuan WL,Marshall PJ,Carman M,Tomaselli K,Lieberburg I,Kagan BL

更新日期：1994-07-29 00:00:00

abstract：:Xylan from Rhodymenia palmata binds to the cellobiohydrolase I from Trichoderma reesei (CBH I) or its core protein, inhibiting their activity. Adsorption onto microcrystalline cellulose (Avicel) is reduced approximately 30% for intact CBH I and nearly 50% for the core, whereas the effects with cellobiose are negligibl...

journal_title：Biochemical and biophysical research communications

authors： Abuja PM,Pilz I,Tomme P,Claeyssens M

更新日期：1989-12-15 00:00:00

abstract：:The effects of etomoxir, an irreversible carnitine palmitoyltransferase I inhibitor, on the liver protein pattern and on liver morphology were examined by two-dimensional gel electrophoresis in female Sprague-Dawley rats treated with 125 mg/kg/day etomoxir for 28 days. In livers of treated animals a protein spot was f...

journal_title：Biochemical and biophysical research communications

authors： Steiner S,Wahl D,Mangold BL,Robison R,Raymackers J,Meheus L,Anderson NL,Cordier A

更新日期：1996-01-26 00:00:00

abstract：:Lp-A-I was isolated by immunoaffinity chromatography and then separated into two fractions of large and small Lp-A-I particles by conventional ultracentrifugation with a cut-off density of 1.125 g/ml. The large and small particle-rich fractions were then radiolabeled with [125I]-Na and [131I]-Na, respectively. Both of...

journal_title：Biochemical and biophysical research communications

authors： Saku K,Liu R,Ohta T,Jimi S,Matsuda I,Arakawa K

更新日期：1994-04-15 00:00:00

abstract：:Nitrogen heterocycle small molecules display various pharmaceutically important bioactivities and have great potential in drug development and application. Microbes are an important source for discovering nitrogen heterocycle natural products, and the elucidation of their biosynthetic pathways in microbes facilitates ...

journal_title：Biochemical and biophysical research communications

authors： Chai S,Cao M,Feng Z

更新日期：2021-01-11 00:00:00

abstract：:The human pregnane X receptor (hPXR) plays a key role in the regulation of both drug metabolism and efflux by inducing the expression of CYP3A4 and MDR1 gene. Using reverse transcription-polymerase chain reaction (RT-PCR) analysis, we identified seven novel splicing variants of hPXR in tissue from a single human liver...

journal_title：Biochemical and biophysical research communications

authors： Fukuen S,Fukuda T,Matsuda H,Sumida A,Yamamoto I,Inaba T,Azuma J

更新日期：2002-11-01 00:00:00

abstract：:Application of landscape theory and the dehydron hypothesis to a crystal structure of a G85R mutant superoxide dismutase (SOD1) tetrameric complex allows for the description of a prion-like hypothesis that serves to explain propagated SOD1 misfolding. We have developed two conformational-change scenarios, one local to...

journal_title：Biochemical and biophysical research communications

authors： Healy EF

更新日期：2016-09-30 00:00:00

abstract：:Colorectal cancer is a common cancer with metachronous distant metastases still threatening overall survival. Tumor oxygen level influences tumor radiosensitivity in relation to autophagy and apoptosis. The objective of this study is to evaluate the expression and interaction between multiple key regulators in differe...

journal_title：Biochemical and biophysical research communications

authors： Tam SY,Wu VWC,Law HKW

更新日期：2019-09-17 00:00:00