Channel formation in planar lipid bilayers by a neurotoxic fragment of the beta-amyloid peptide.

abstract：:T-type Ca(2+) channels are important regulators of peripheral sensory neuron excitability. Accordingly, T-type Ca(2+) currents are often increased in various pathological pain conditions, such as inflammation or nerve injury. Here we investigated effects of inflammation on functional expression of T-type Ca(2+) channe...

journal_title：Biochemical and biophysical research communications

authors： Huang D,Liang C,Zhang F,Men H,Du X,Gamper N,Zhang H

更新日期：2016-04-29 00:00:00

abstract：:Hepatitis delta virus (HDV) is a human pathogen causing fulminant hepatitis and liver cirrhosis. HDV has a circular single-stranded RNA genome, which encodes only a single protein, hepatitis delta antigen (HDAg), from the antigenomic strand. Although the functional roles of HDAg have been extensively studied, the mole...

journal_title：Biochemical and biophysical research communications

authors： Hwang SB,Park KJ,Kim YS

更新日期：1998-03-27 00:00:00

abstract：:The stage V sporulation protein G (SpoVG) homolog of Staphylococcus aureus is a modulator of virulence factor synthesis and antibiotic resistance in this clinically important gram-positive pathogen. Here we demonstrate that SpoVG can be phosphorylated by the staphylococcal Ser/Thr protein kinase Stk1 and that phosphor...

journal_title：Biochemical and biophysical research communications

authors： Bischoff M,Brelle S,Minatelli S,Molle V

更新日期：2016-05-13 00:00:00

abstract：:Putative E2/NS1 sequence of hepatitis C virus was expressed in E. coli as a fusion protein with maltose binding protein. Approximately 80 kDa protein was obtained containing 38 kDa E2/NS1 protein. The antibody to this protein was detectable in the same serum from which the sequence was amplified. It was also detectabl...

journal_title：Biochemical and biophysical research communications

authors： Yokosuka O,Ito Y,Imazeki F,Ohto M,Omata M

更新日期：1992-11-30 00:00:00

abstract：:Rats were fed for 15 days a diet containing ammonium acetate (20% w/w) and then injected i. p. with ammonium acetate (7 mmol/Kg). Only 1 out of 18 control rats but 9 of 18 rats fed ammonium survived, indicating a protective effect of ammonium ingestion against an acute ammonia challenge. Blood ammonia returned to norm...

journal_title：Biochemical and biophysical research communications

authors： Miñana MD,Felipo V,Grisolía S

更新日期：1988-06-30 00:00:00

abstract：:Fibroblast growth factor (FGF)-23 was identified as a causative factor of tumor-induced osteomalacia and also as a responsible gene for autosomal dominant hypophosphatemic rickets. To clarify the pathophysiological roles of FGF-23 in these diseases, we generated its transgenic mice. The transgenic mice expressing huma...

journal_title：Biochemical and biophysical research communications

authors： Shimada T,Urakawa I,Yamazaki Y,Hasegawa H,Hino R,Yoneya T,Takeuchi Y,Fujita T,Fukumoto S,Yamashita T

更新日期：2004-02-06 00:00:00

abstract：OBJECTIVE:The aim of this study was to determine whether ATP-binding cassette transporter A1 (ABCA1) was up-regulated by growth differentiation factor-15 (GDF-15) via the phosphoinositide 3-kinase (PI3K)/protein kinase Cζ (PKCζ)/specificity protein 1 (SP1) pathway in THP-1 macrophages. METHODS AND RESULTS:We investiga...

journal_title：Biochemical and biophysical research communications

authors： Wu JF,Wang Y,Zhang M,Tang YY,Wang B,He PP,Lv YC,Ouyang XP,Yao F,Tan YL,Tang SL,Tang DP,Cayabyab FS,Zheng XL,Zhang DW,Zeng GF,Tang CK

更新日期：2014-02-14 00:00:00

abstract：:7,8-Dihydrobiopterin is not an intermediate in the de novo biosynthesis of tetrahydrobiopterin, the cofactor required for aromatic amino acid hydroxylations. However, N-acetyl-serotonin inhibition of sepiapterin reductase, an enzyme whose previously only known function was the reduction of sepiapterin to 7,8-dihydrobi...

journal_title：Biochemical and biophysical research communications

authors： Milstien S,Kaufman S

更新日期：1983-09-30 00:00:00

abstract：:The small heat shock protein hsp27 is associated with aggressive tumor behavior in certain subsets of breast cancer patients. Previously we demonstrated that hsp27 overexpression in breast cancer cells increased in vitro and in vivo invasiveness, suggesting that hsp27 influences the metastatic process. To investigate ...

journal_title：Biochemical and biophysical research communications

authors： Hansen RK,Parra I,Hilsenbeck SG,Himelstein B,Fuqua SA

更新日期：2001-03-23 00:00:00

abstract：:Nuclei were isolated from bloodstream forms of Trypanosoma brucei by nitrogen cavitation and sedimentation through percoll density gradients. Transcription studies with these nuclei in vitro demonstrated features not seen with other eukaryotes: RNA synthesis was much greater in the presence of Mn2+ than with Mg2+ and ...

journal_title：Biochemical and biophysical research communications

authors： Earnshaw DL,Beebee TJ,Gutteridge WE

更新日期：1985-09-16 00:00:00

abstract：:Isolated Agrobacterium tumefaciens was exposed to different extremely low frequencies of square amplitude modulated waves (QAMW) from two generators to determine the resonance frequency that causes growth inhibition. The carrier was 10 MHz sine wave with amplitude ±10 Vpp which was modulated by a second wave generator...

journal_title：Biochemical and biophysical research communications

authors： Fadel MA,El-Gebaly RH,Mohamed SA,Abdelbacki AMM

更新日期：2017-12-09 00:00:00

abstract：:Electroporation is the most widely employed method of gene transfer into macrophages which are hard to transfect. RAW 264.7 is a widely used cell line for studying macrophage responses. Electroporation of RAW 264.7 cells with commercial reagents although very efficient is expensive necessitating the development of cos...

journal_title：Biochemical and biophysical research communications

authors： Alamuru-Yellapragada NP,Kapadia B,Parsa KVL

更新日期：2017-05-27 00:00:00

abstract：:The process in which nucleotide is buried into the active site of heavy meromyosin was studied with stopped-flow apparatus by monitoring the time-course of the large fluorescence increase of 1,N6-ethenoadenosine triphosphate (epsilon-ATP) when it binds from acrylamide-containing solutions. We have recently reported th...

journal_title：Biochemical and biophysical research communications

authors： Ando T,Duke JA

更新日期：1983-08-30 00:00:00

abstract：:Extra long PCR analysis of mitochondrial DNA (mtDNA) isolated from skeletal muscle of humans of different ages revealed three phenomena: (i) the amount of normal length mtDNA (16.5 kb) was progressively reduced with age, such that the cells of old age individuals (about 90 years) contained little, or undetectable amou...

journal_title：Biochemical and biophysical research communications

authors： Kovalenko SA,Kopsidas G,Kelso JM,Linnane AW

更新日期：1997-03-06 00:00:00

abstract：:Human LPTS/PinX1 is a telomerase-inhibitory protein, which binds to the telomere protein Pin2/TRF1 and the catalytic subunit hTERT of telomerase. To explore the proteins that might be involved in the telomerase pathway, we performed a yeast two-hybrid screening with LPTS/PinX1 as the bait. A novel gene, MCRS2, encodin...

journal_title：Biochemical and biophysical research communications

authors： Song H,Li Y,Chen G,Xing Z,Zhao J,Yokoyama KK,Li T,Zhao M

更新日期：2004-04-16 00:00:00

abstract：:The crypt-villi axis of intestinal mucosa maintains homeostasis by renewal of epithelia, and also exhibits different properties from undifferentiated to terminally differentiated cells. We investigated differential susceptibility to genotoxin-induced cell death, based on the degree of differentiation of epithelial cel...

journal_title：Biochemical and biophysical research communications

authors： Chae B,Yang KM,Kim TI,Kim WH

更新日期：2009-01-23 00:00:00

abstract：:Coxsackievirus A24 (CVA24) is responsible for acute hemorrhagic conjunctivitis, a highly contagious eye disease for which no prevention or treatment is currently available. We thus assessed the antiviral potential of a small interfering RNA (siRNA) targeting CVA24. HeLa cells with or without four different siRNAs comp...

journal_title：Biochemical and biophysical research communications

authors： Jun EJ,Nam YR,Ahn J,Tchah H,Joo CH,Jee Y,Kim YK,Lee H

更新日期：2008-11-14 00:00:00

abstract：:Iron overload, as a risk factor for osteoporosis, can result in the up-regulation of Hepcidin, and Hepcidin knockout mice display defects in their bone microarchitecture. However, the molecular and genetic mechanisms underlying Hepcidin deficiency-derived bone loss remain unclear. Here, we show that hepcidin knockdown...

journal_title：Biochemical and biophysical research communications

authors： Jiang Y,Yan Y,Wang X,Zhu G,Xu YJ

更新日期：2016-07-15 00:00:00

abstract：:Inositol 1,2,3,5,6-pentakisphosphate (Ins(1,2,3,5,6)P5) 5-phosphatase present in fetal calf thymus has been partially purified. This enzyme was inhibited dose-dependently by different thiol modifiers like N-ethylmaleimide (NEM), p-chloromercuribenzene sulfonate (PCMBS), diamide, and phenylarsine oxide (PAO). The inhib...

journal_title：Biochemical and biophysical research communications

authors： Bandyopadhyay U,Kaiser T,Rudolf MT,Schultz C,Guse AH,Mayr GW

更新日期：1997-11-07 00:00:00

abstract：:To gain further insight into the biochemical properties of the antibacterial hexetidine, isolated rat liver mitochondria were added with this drug and investigation made of certain features related to mitochondrial bioenergetics. Hexetidine was found to cause oxidation of intramitochondrial pyridine nucleotides and st...

journal_title：Biochemical and biophysical research communications

authors： D'Arcangelo G,Barile M,Passarella S,Quagliariello E

更新日期：1987-09-15 00:00:00

abstract：:The ZntR protein from Escherichia coli is a member of the MerR-family of transcriptional regulatory proteins and acts as a hyper-sensitive transcriptional switch primarily in response to Zn(II) and Cd(II). The binding of metal-ions to ZntR initiates a mechanism that remodels the cognate promoter, increasing its affini...

journal_title：Biochemical and biophysical research communications

authors： Khan S,Brocklehurst KR,Jones GW,Morby AP

更新日期：2002-12-06 00:00:00

abstract：:Dietary phosphate restriction produces an adaptive increase in renal tubular Na/Pi cotransport. A similar adaptation occurs during phosphate depletion in opossum kidney cells, a continuous line of cultured renal epithelial cells. We investigated the cellular changes associated with adaptation to phosphate depletion in...

journal_title：Biochemical and biophysical research communications

authors： Saxena S,Dansby L,Allon M

更新日期：1995-11-02 00:00:00

abstract：:Chloride intracellular channel protein CLIC4 is a putative organellar anion channel or channel regulator with an unusual dual cytoplasmic and integral membrane localisation. To investigate its contribution to cellular anion channel activity, the protein was overexpressed in stably transfected HEK-293 cells. Patch-clam...

journal_title：Biochemical and biophysical research communications

authors： Proutski I,Karoulias N,Ashley RH

更新日期：2002-09-20 00:00:00

abstract：:In this report, we demonstrate that HL-60 nuclei isolated in calcium but not EGTA containing buffers specifically bind PE and express approximately 37,000 receptor sites/nucleus. Nuclear phorbol ester binding is lost by isolation in the absence of calcium, but can be repleted by the addition of partially purified prot...

journal_title：Biochemical and biophysical research communications

authors： Kraft AS,Appling C,Berkow RL

更新日期：1987-04-14 00:00:00

abstract：:In this study we investigated the role of active site residues in the peroxidase activity of Orp1 (GPx3) using three different peroxide substrates. Using a structural homology model of the reduced form of Orp1, we identified Asn126 and Phe127 as evolutionarily conserved residues that line the back of the Orp1 active s...

journal_title：Biochemical and biophysical research communications

authors： Takanishi CL,Ma LH,Wood MJ

更新日期：2010-12-03 00:00:00

abstract：:Daudi lymphoma cells, of a line sensitive to growth inhibition by alpha interferon, showed dose-dependent plasma membrane depolarization within 10 min after exposure to natural or recombinant alpha interferons (10 to 1000 IU/ml). This biophysical change was detected flow cytometrically by measuring the intensity of fl...

journal_title：Biochemical and biophysical research communications

authors： Grimley PM,Aszalos A

更新日期：1987-07-15 00:00:00

abstract：:We generated two transgenic mice carrying the adenovirus type 12 E1 region genes under control of the human renin promoter, in which the E1A and E1B genes were expressed predominantly in the kidney. Interestingly, renal transcription of the gene for mouse renin was shown to be elevated at 28 and 40 folds as compared i...

journal_title：Biochemical and biophysical research communications

authors： Fukamizu A,Sagara M,Sugiyama F,Yagami K,Murakami K

更新日期：1994-02-28 00:00:00

abstract：:Matrix metalloproteinases (MMPs) have both protective and pathological roles in inflammation, and transcriptional mechanisms are important in regulating physiological levels to maintain health. Zinc-binding protein-89 (ZBP-89) is a transcription factor with roles in regulating vital cellular processes, acting through ...

journal_title：Biochemical and biophysical research communications

authors： Borghaei RC,Gorski G,Seutter S,Chun J,Khaselov N,Scianni S

更新日期：2016-03-18 00:00:00

abstract：:Protein folding continues to be an important biophysical topic in molecular biology. We report the parameters for successfully refolding the guanylyl cyclase core of the ANP receptor, an allosteric homodimeric enzyme. Urea was a better chaotropic solvent than guanidine HCl, and physiological salt concentrations and pH...

journal_title：Biochemical and biophysical research communications

authors： Thorpe DS,Niu S,Morkin E

更新日期：1996-07-25 00:00:00

abstract：:Virosomes as membranous vesicles with viral fusion protein in their membrane are versatile vehicles for cargo delivery. The vesicular stomatitis virus glycoprotein (VSV-G) is a common fusogenic protein used in virosome preparation. This glycoprotein has been used in liposomal systems so far, but in this study, we have...

journal_title：Biochemical and biophysical research communications

authors： Asadikaram G,Poustforoosh A,Pardakhty A,Torkzadeh-Mahani M,Nematollahi MH

更新日期：2021-01-01 00:00:00