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Identification of the novel splicing variants for the hPXR in human livers.

Abstract：

:The human pregnane X receptor (hPXR) plays a key role in the regulation of both drug metabolism and efflux by inducing the expression of CYP3A4 and MDR1 gene. Using reverse transcription-polymerase chain reaction (RT-PCR) analysis, we identified seven novel splicing variants of hPXR in tissue from a single human liver. The expression of hPXR-related transcripts in the liver samples of 15 Caucasian individuals was subsequently determined by RT-PCR assays. The pattern of expression levels of these transcripts varied among liver samples. These results suggest that the hPXR is expressed as several different transcripts in liver tissues, apparently due to alternative as well as defective gene splicing. Furthermore, because this study provides the possibility of interindividual differences in hPXR transcript profiles, these alternative splicings for hPXR may largely contribute to the interindividual variability in CYP3A4 and P-glycoprotein induction.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Fukuen S,Fukuda T,Matsuda H,Sumida A,Yamamoto I,Inaba T,Azuma J

doi

10.1016/s0006-291x(02)02469-5

subject

Has Abstract

pub_date

2002-11-01 00:00:00

pages

433-8

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006291X02024695

journal_volume

298

pub_type

杂志文章

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