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F0F1-ATPase as biosensor to detect single virus.

Abstract：

:F(0)F(1)-ATPase within chromatophore was constructed as a biosensor (immuno-rotary biosensor) for the purpose of capturing single virus. Capture of virus was based on antibody-antigen reaction. The detection of virus based on proton flux change driven by ATP-synthesis of F(0)F(1)-ATPase, which was indicated by F1300, was directly observed by a fluorescence microscope. The results demonstrate that the biosensor loading of virus particles has remarkable signal-to-noise ratio (3.8:1) compared to its control at single molecular level, and will be convenient, quick, and even super-sensitive for detecting virus particles.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Liu X,Zhang Y,Yue J,Jiang P,Zhang Z

doi

10.1016/j.bbrc.2006.02.103

subject

Has Abstract

pub_date

2006-04-21 00:00:00

pages

1319-22

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(06)00357-3

journal_volume

342

pub_type

杂志文章

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