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Protein fragment complementation in M.HhaI DNA methyltransferase.

Abstract：

:The 5mC DNA methyltransferase M.HhaI can be split into two individually inactive N- and C-terminal fragments that together can form an active enzyme in vivo capable of efficiently methylating DNA. This active fragment pair was identified by creating libraries of M.HhaI gene fragment pairs and then selecting for the pairs that code for an active 5mC methyltransferase. The site of bisection for successful protein fragment complementation in M.HhaI was in the variable region near the target recognition domain between motif VIII and TRD. This same region is the location of bifurcation in the naturally split 5mC methyltransferase M.AquI, the location for circular permutation in M.BssHII, and the location for previously engineered split versions of M.BspRI.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Choe W,Chandrasegaran S,Ostermeier M

doi

10.1016/j.bbrc.2005.07.017

subject

Has Abstract

pub_date

2005-09-09 00:00:00

pages

1233-40

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(05)01457-9

journal_volume

334

pub_type

杂志文章

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