当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Newly expressed proteins of mouse embryonic fibroblasts irradiated to be inactive.

Newly expressed proteins of mouse embryonic fibroblasts irradiated to be inactive.

Abstract：

:It has been found that post-radiation mouse embryonic fibroblasts can well maintain the pluripotency in human embryonic stem cells. However, the molecular mechanism remains unclear. In the present study, the new protein expression profile of post-radiation mouse embryonic fibroblasts was analyzed by immobilized pH gradient 2-dimensional polyacrylamide gel electrophoresis. Image analysis following silver staining revealed (969+/-57) vs. (1085+/-107) spots from post-radiation mouse embryonic fibroblasts and pre-radiation ones, respectively. Some newly expressed proteins, which were only abundantly present after irradiation, were subjected to peptide mass fingerprint analysis and identified using MALDI-TOF-MS, SWISS-PROT database, and RT-PCR. Several of those proteins were preliminarily identified to participate in cytokine secretion, cell signal transduction, transcriptional regulation, and apoptosis, etc., which suggested that inactive post-radiation mouse embryonic fibroblasts expressed some new proteins that may underlie the molecular mechanisms to maintain the pluripotency in human embryonic stem cells.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Xie CQ,Lin G,Luo KL,Luo SW,Lu GX

doi

10.1016/j.bbrc.2004.01.089

subject

Has Abstract

pub_date

2004-03-12 00:00:00

pages

581-8

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006291X04001160

journal_volume

315

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • TNP-470 blockage of VEGF synthesis is dependent on MAPK/COX-2 signaling pathway in PDGF-BB-activated hepatic stellate cells.

    abstract：:Angiogenesis is a key pathogenic event in hepatic fibrogenesis, which is mediated by activated hepatic stellate cells (HSCs). TNP-470 is a known anti-angiogenic agent in cancer, and in this study, we investigated the regulatory mechanisms of TNP-470 blockage of vascular endothelial growth factor (VEGF) synthesis in ac...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.12.175

    authors： Wang YQ,Luk JM,Chu AC,Ikeda K,Man K,Kaneda K,Fan ST

    更新日期：2006-03-03 00:00:00

  • Anabolic function of the type II isozyme of hexokinase in hepatic lipid synthesis.

    abstract：:The mRNA encoding the Type II isozyme of hexokinase was markedly elevated in livers of transgenic mice overexpressing the transcriptionally active nuclear form of sterol regulatory element binding protein-1a (nSREBP-1a), but not in transgenic mice overexpressing the nSREBP-1c or nSREBP-2 isoforms. Cellulose acetate el...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2527

    authors： Sebastian S,Horton JD,Wilson JE

    更新日期：2000-04-21 00:00:00

  • Tropylium tetrafluoroborate, a novel substrate for aldehyde oxidase.

    abstract：:The stabilized carbonium ion salt, tropylium tetrafluoroborate, was oxidized to tropone (cycloheptatrienone) by rabbit liver aldehyde oxidase but not by the closely related molybdenum hydroxylase, xanthine oxidase. The tropylium cation is an aromatic hydrocarbon which lacks the aldehyde, imine, or iminium functional g...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90775-8

    authors： Banks RB,Barnett SD

    更新日期：1986-10-30 00:00:00

  • The novel extremely psychrophilic luciferase from Metridia longa: Properties of a high-purity protein produced in insect cells.

    abstract：:The bright bioluminescence of copepod Metridia longa is conditioned by a small secreted coelenterazine-dependent luciferase (MLuc). To date, three isoforms of MLuc differing in length, sequences, and some properties were cloned and successfully applied as high sensitive bioluminescent reporters. In this work, we repor...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.12.067

    authors： Larionova MD,Markova SV,Vysotski ES

    更新日期：2017-01-29 00:00:00

  • Trichosanthin affects HSV-1 replication in Hep-2 cells.

    abstract：:Trichosanthin (TCS) is a type I ribosome-inactivating protein that inhibits the replication of both human immunodeficiency virus type 1 (HIV-1) and herpes simplex virus type 1 (HSV-1). The mechanism of inhibition is not clear. This investigation explored the effects of TCS on the stages of HSV-1 infection in Hep-2 cel...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.10.080

    authors： He DX,Tam SC

    更新日期：2010-11-26 00:00:00

  • Germ cell nuclear factor is a response element-specific repressor of transcription.

    abstract：:We have shown that the orphan receptor Germ Cell Nuclear Factor (GCNF) binds to a direct repeat of the sequence AGGTCA with zero base pair spacing (DR0). Here, we further characterize the binding characteristics of GCNF. We demonstrate that GCNF binds specifically to DR0s as a homodimer, and does not bind with high af...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8391

    authors： Cooney AJ,Hummelke GC,Herman T,Chen F,Jackson KJ

    更新日期：1998-04-07 00:00:00

  • Osteoclastic activity induces osteomodulin expression in osteoblasts.

    abstract：:Bone resorption by osteoclasts stimulates bone formation by osteoblasts. To isolate osteoblastic factors coupled with osteoclast activity, we performed microarray and cluster analysis of 8 tissues including bone, and found that among 10,490 genes, osteomodulin (OMD), an extracellular matrix keratan sulfate proteoglyca...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.07.193

    authors： Ninomiya K,Miyamoto T,Imai J,Fujita N,Suzuki T,Iwasaki R,Yagi M,Watanabe S,Toyama Y,Suda T

    更新日期：2007-10-19 00:00:00

  • Small molecule antagonists of CCR8 inhibit eosinophil and T cell migration.

    abstract：:In this study, we demonstrate that in addition to T lymphocytes, human naïve eosinophils and the differentiated eosinophil-like cell line, AML14.3D10 express CCR8 and respond to CCL1 through CCR8 engagement. The responsiveness of cells was dependent on maturation stage, since CCL1 induced pronounced chemotaxis only in...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.03.097

    authors： Karlsson AK,Walles K,Bladh H,Connolly S,Skrinjar M,Rosendahl A

    更新日期：2011-04-22 00:00:00

  • Estrogen modulation of endothelium-derived relaxing factors by human endothelial cells.

    abstract：:We report the modulatory effects of estrogen on release of endothelium-derived relaxing factors (EDRFs) in a human endothelial cell line, EA.hy926. Using bioassay, we showed that EA.hy926 released EDRF including nitric oxide (NO) and endothelium-derived hyperpolarizing factor (EDHF) measured by relaxation of pre-contr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.07.137

    authors： Rahimian R,Chan L,Goel A,Poburko D,van Breemen C

    更新日期：2004-09-17 00:00:00

  • Antiproliferative effect of 1,25-dihydroxyvitamin D3 and its analogs on human colon adenocarcinoma cells (CaCo-2): influence of extracellular calcium.

    abstract：:Depending on culture in either "low Ca++" (0.25 mM) or "normal Ca++" (1.8 mM) medium, human colon adenocarcinoma-derived CaCo-2 cells exhibit differential sensitivity to the antiproliferative action of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) and of two side-chain modified analogs, 1,25S,26-trihydroxy-delta 22-vitamin D...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91333-8

    authors： Cross HS,Huber C,Peterlik M

    更新日期：1991-08-30 00:00:00

  • F0F1-ATPase as biosensor to detect single virus.

    abstract：:F(0)F(1)-ATPase within chromatophore was constructed as a biosensor (immuno-rotary biosensor) for the purpose of capturing single virus. Capture of virus was based on antibody-antigen reaction. The detection of virus based on proton flux change driven by ATP-synthesis of F(0)F(1)-ATPase, which was indicated by F1300, ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.02.103

    authors： Liu X,Zhang Y,Yue J,Jiang P,Zhang Z

    更新日期：2006-04-21 00:00:00

  • Exposure of protein kinase motifs that trigger binding of Hsp90 and Cdc37.

    abstract：:Hsp90 and its co-chaperone Cdc37 are required for the activity of numerous eukaryotic protein kinases. c-Jun N-terminal kinases (JNKs) appear to be Hsp90-independent kinases, as their activity is unaffected by Hsp90 inhibition. It is currently unknown why some protein kinases are Hsp90- and Cdc37-dependent for their f...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.10.100

    authors： Prince T,Matts RL

    更新日期：2005-12-23 00:00:00

  • Quantum dots in bio-imaging: Revolution by the small.

    abstract：:Visual analysis of biomolecules is an integral avenue of basic and applied biological research. It has been widely carried out by tagging of nucleotides and proteins with traditional fluorophores that are limited in their application by features such as photobleaching, spectral overlaps, and operational difficulties. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2005.02.043

    authors： Arya H,Kaul Z,Wadhwa R,Taira K,Hirano T,Kaul SC

    更新日期：2005-04-22 00:00:00

  • Construction of a cDNA clone containing the entire coding region of the human liver-type phosphofructokinase.

    abstract：:Genomic fragments containing coding sequences of the human liver phosphofructokinase (PFKL) were used to screen cDNA libraries and several clones corresponding to PFKL were isolated. Three overlapping cDNA clones spanning the entire coding region were stitched together yielding the plasmid pG-cPFKL3.0 containing a 3Kb...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(87)80194-8

    authors： Levanon D,Danciger E,Dafni N,Groner Y

    更新日期：1987-09-30 00:00:00

  • α-Synuclein accumulation reduces GABAergic inhibitory transmission in a model of multiple system atrophy.

    abstract：:Multiple system atrophy is a neurodegenerative disease caused by abnormal α-synuclein (α-syn) accumulation in oligodendrocytes and neurons. We previously demonstrated that transgenic (Tg) mice that selectively overexpressed human α-syn in oligodendrocytes exhibited neuronal α-syn accumulation. Microtubule β-III tubuli...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.10.057

    authors： Ito H,Nakayama K,Jin C,Suzuki Y,Yazawa I

    更新日期：2012-11-23 00:00:00

  • Folate-modified PLGA nanoparticles for tumor-targeted delivery of pheophorbide a in vivo.

    abstract：:Targeted drug delivery has been an important issue for tumor therapy including photodynamic therapy (PDT). The purpose of our study is to increase the targeting efficiency of photosensitizer (PS) using folate-modified nanoparticles (NPs) to tumor site in vivo. Folate receptor is over-expressed on the surface of many h...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.03.013

    authors： Son J,Yang SM,Yi G,Roh YJ,Park H,Park JM,Choi MG,Koo H

    更新日期：2018-04-06 00:00:00

  • Specific desensitization of actin polymerization of bovine platelets.

    abstract：:Polymerization of actin induced by activation of platelets was investigated using deoxyribonuclease I inhibition assay. When platelets were activated with ADP or 5-hydroxytryptamine, actin was polymerized quickly followed by rapid depolymerization to the initial level. Reactivation with the same agonist, however, did ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(84)80184-9

    authors： Imada T,Kikuchi T,Shimada H,Inada Y,Saito Y

    更新日期：1984-05-16 00:00:00

  • The specific bisindolylmaleimide PKC-inhibitor GF 109203X efficiently modulates MRP-associated multiple drug resistance.

    abstract：:The newly identified drug transporter MRP is functionally linked to a multiple drug resistance independent from P-glycoprotein. Resistance modifiers for this type of MDR are rare at present. We analyzed the modulating effect of the highly selective bisindolylmaleimide PKC inhibitor GF 109203X on the MRP overexpressing...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1017

    authors： Gekeler V,Boer R,Ise W,Sanders KH,Schächtele C,Beck J

    更新日期：1995-01-05 00:00:00

  • Efficient generation of transgenic mice by direct intraovarian injection of plasmid DNA.

    abstract：:We established a rapid procedure for obtaining transgenic mice by directly injecting an enhanced green fluorescent protein (EGFP)-expressing plasmid (pIRES-EGFP) into the ovaries of fertile mice. The frequency of transgenic mouse production was determined by pair-mating, and by polymerase chain reaction (PCR) and sequ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.04.112

    authors： Yang SY,Wang JG,Cui HX,Sun SG,Li Q,Gu L,Hong Y,Liu PP,Liu WQ

    更新日期：2007-06-22 00:00:00

  • Mutation of the carboxy terminal zinc finger of E. coli isoleucyl-tRNA synthetase alters zinc binding and aminoacylation activity.

    abstract：:Escherichia coli isoleucyl-tRNA synthetase has been shown to contain two enzyme-bound zinc atoms per polypeptide chain. To investigate the structural and functional significance of the C-terminal enzyme-bound zinc, mutagenesis was used to alter Cys 922 to Ser [IleRS(C922S)] and to replace Cys 922 through Ala 939 with ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2671

    authors： Zhou L,Rosevear PR

    更新日期：1995-11-13 00:00:00

  • Cleavage of cottontail rabbit papillomavirus E7 RNA with an anti-E7 ribozyme.

    abstract：:We have designed and constructed a plasmid (pE7RZ-1) that contains a gene for a hammerhead ribozyme that specifically cleaves cottontail rabbit papillomavirus (CRPV) E7 RNA sequences in vitro. The in vitro produced transcripts of pE7RZ-1 cleave CRPV E7 target sequences in trans at 37 degrees C. Pretreatment of pE7RZ-1...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1490

    authors： Wisotzkey JD,Krizenoskas A,DiAngelo S,Kreider JW

    更新日期：1993-04-30 00:00:00

  • Reconstitution of the NF1 GAP-related domain in NF1-deficient human Schwann cells.

    abstract：:Schwann cells derived from peripheral nerve sheath tumors from individuals with Neurofibromatosis Type 1 (NF1) are deficient for the protein neurofibromin, which contains a GAP-related domain (NF1-GRD). Neurofibromin-deficient Schwann cells have increased Ras activation, increased proliferation in response to certain ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.07.159

    authors： Thomas SL,Deadwyler GD,Tang J,Stubbs EB Jr,Muir D,Hiatt KK,Clapp DW,De Vries GH

    更新日期：2006-09-29 00:00:00

  • Prompt activation of telomerase by chemical carcinogens in rats detected with a modified TRAP assay.

    abstract：:The maintenance of telomere length is crucial for survival of cells. Telomerase is an RNA-containing reverse transcriptase, which is responsible for elongation of shortened telomeres. Telomerase reactivation has been suggested to be involved in malignant progressions. To study on the involvement of telomerase activati...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8487

    authors： Miura M,Karasaki Y,Abe T,Higashi K,Ikemura K,Gotoh S

    更新日期：1998-05-08 00:00:00

  • Identification of ubiquitin-modified lysine residues and novel phosphorylation sites on eukaryotic initiation factor 2B epsilon.

    abstract：:Eukaryotic initiation factor 2Bε (eIF2Bε) plays a critical role in the initiation of mRNA translation and its expression and guanine nucleotide exchange activity are major determinants of the rate of protein synthesis. In this work we provide evidence that the catalytic epsilon subunit of eIF2B is subject to ubiquitin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.05.053

    authors： Tuckow AP,Kazi AA,Kimball SR,Jefferson LS

    更新日期：2013-06-21 00:00:00

  • Vitamin D fails to prevent serum starvation- or staurosporine-induced apoptosis in human and rat osteosarcoma-derived cell lines.

    abstract：:Previous studies have suggested that 1,25(OH)2D3, the active form of vitamin D3, may increase the survival of bone-forming osteoblasts through an inhibition of apoptosis. On the other hand, vitamin D3 has also been shown to trigger apoptosis in human cancer cells, including osteosarcoma-derived cell lines. In the pres...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.03.061

    authors： Witasp E,Gustafsson AC,Cotgreave I,Lind M,Fadeel B

    更新日期：2005-05-13 00:00:00

  • Cloning and characterization of the human stearoyl-CoA desaturase gene promoter: transcriptional activation by sterol regulatory element binding protein and repression by polyunsaturated fatty acids and cholesterol.

    abstract：:Stearoyl-CoA desaturase (SCD) is a microsomal enzyme required for the biosynthesis of oleate (C18:1) and palmitoleate (C16:1) which are the major monounsaturated fatty acids of membrane phospholipids, triglycerides and cholesterol esters. Previously the full-length human skin cDNA was sequenced and the exon and intron...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5102

    authors： Bené H,Lasky D,Ntambi JM

    更新日期：2001-06-29 00:00:00

  • Computer studies on the stereostructure and quantum chemical properties of 6-pyruvoyl tetrahydropterin, the key intermediate of tetrahydrobiopterin biosynthesis.

    abstract：:The optimized geometry of the conformation of atoms constituting the 6-pyruvoyl tetrahydropterin molecule, the labile key intermediate of tetrahydrobiopterin biosynthesis, was obtained by molecular orbital calculations within the MINDO/3 framework. The stereostructure of the molecule showing the preferred mode for bin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)90888-e

    authors： Katoh S,Sueoka T,Kurihara T

    更新日期：1991-04-15 00:00:00

  • Histone H2A mobility is regulated by its tails and acetylation of core histone tails.

    abstract：:Histone tail domains play important roles in cellular processes, such as replication, transcription, and chromosome condensation. Histone H2A has one central and two tail domains, and their functions have mainly been studied from a biochemical perspective. In addition, analyses based on visualization have been employe...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.03.203

    authors： Higashi T,Matsunaga S,Isobe K,Morimoto A,Shimada T,Kataoka S,Watanabe W,Uchiyama S,Itoh K,Fukui K

    更新日期：2007-06-08 00:00:00

  • Further evidence for both functional and structural microcompartmentation within the membranes of two associated organelles, mitochondrion and endoplasmic reticulum.

    abstract：:We previously demonstrated that the translocation of phospholipids between the mitochondrion and the endoplasmic reticulum occurs via highly specialized membrane microdomains of both organelles that are in situ closely associated. As understanding of the interactions between both organelles requires characterization o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.2194

    authors： Gasnier F,Ardail D,Febvay G,Simonot C,Lerme F,Guillaud J,Louisot P,Gateau-Roesch O

    更新日期：1993-09-30 00:00:00

  • The novel aldehyde dehydrogenase gene, ALDH5, encodes an active aldehyde dehydrogenase enzyme.

    abstract：:The mRNA for the novel aldehyde dehydrogenase 5 (ALDH5) gene was detected in HuH7 hepatoma cells. The cells also expressed cytosolic aldehyde dehydrogenase (ALDH1) mRNA, but no mitochondrial aldehyde dehydrogenase (ALDH2) mRNA. Extracts of the hepatoma cells contained an enzymatic activity with an isoelectric point si...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1789

    authors： Stewart MJ,Malek K,Xiao Q,Dipple KM,Crabb DW

    更新日期：1995-06-06 00:00:00