Soluble multimer of recombinant endostatin expressed in E. coli has anti-angiogenesis activity.


:The bioactivity, refolding, and multimer formation of endostatin, particularly of recombinant endostatin produced from bacteria, are proved challenging for clinical application. In order to determine the biological activity of recombinant endostatin multimer, first, we expressed endostatin in Escherichia coli and purified it with ion-exchange chromatography. The purified active protein could elicit multimer formation spontaneously, but still has comparable activity. Aim to determine the anti-angiogenic activity of multimer endostatin, by use of RP-HPLC, we then successfully separated endostatin monomer and multimer for subjecting to anti-angiogenesis assay. The results from CAM (chorioallantoic membrane) inhibition assay showed that both monomer and multimer suppressed CAM vascularization significantly. At the dosage of 0.8 microg, inhibition rates of multimeric and monomeric proteins were about 58% and 38%, respectively. Multimeric endostatin exerted a higher activity than monomeric endostatin (p < 0.05). However, when the protein dosage is less than 0.4 microg/ml, there is no significance between their inhibition rates (p > 0.05), although both of them show a high inhibition effect in contrast to control. The results from HUVEC proliferation assay also showed similar effects at dosages of 0.6 and 1.6 microg/ml, multimer exerted a higher activity on inhibition of HUVEC proliferation comparing with monomer (p < 0.05). In conclusion, our results suggest that endostatin multimer has a comparable or higher bioactivity and multimerization will not affect its bioactivity, implying that endostatin activity is insensitive to structure conformation contributed by disulfide bonds.


Wei DM,Gao Y,Cao XR,Zhu NC,Liang JF,Xie WP,Zhen MY,Zhu MS




Has Abstract


2006-07-14 00:00:00














  • Illegitimate recombination in a bovine papillomavirus shuttle vector: a high level of site specificity.

    abstract::Recombination in a bovine papillomavirus shuttle vector carrying direct repeats of Moloney murine leukemia virus LTR sequence was examined. Differently from similar vectors carrying direct repeats of SV40 polyA addition signal or neomycin resistance gene, the vector exhibited no homologous recombination between the re...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kitamura Y,Naito A,Yoshikura H

    更新日期:1991-08-30 00:00:00

  • Differential interaction of CrkII adaptor protein with platelet-derived growth factor alpha- and beta-receptors is determined by its internal tyrosine phosphorylation.

    abstract::CrkII is an intracellular adaptor protein involved in signal transduction by various growth factors. Activation of PDGF alpha-receptor resulted in its association with CrkII in vivo. In contrast, binding of CrkII to the PDGF beta-receptor was negligible, despite its becoming prominently phosphorylated. Bacterially exp...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Matsumoto T,Yokote K,Take A,Takemoto M,Asaumi S,Hashimoto Y,Matsuda M,Saito Y,Mori S

    更新日期:2000-04-02 00:00:00

  • Cancer associated fibroblasts express pro-inflammatory factors in human breast and ovarian tumors.

    abstract::Inflammation has been established in recent years as a hallmark of cancer. Cancer Associated Fibroblasts (CAFs) support tumorigenesis by stimulating angiogenesis, cancer cell proliferation and invasion. We previously demonstrated that CAFs also mediate tumor-enhancing inflammation in a mouse model of skin carcinoma. B...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Erez N,Glanz S,Raz Y,Avivi C,Barshack I

    更新日期:2013-08-02 00:00:00

  • Identification and characterization of mouse PSF1-binding protein, SLD5.

    abstract::Although most somatic cells cannot proliferate, immature cells proliferate continuously to produce mature cells. Recently, we cloned mouse PSF1 from a hematopoietic stem cell specific cDNA library and reported that PSF1 is indispensable for the proliferation of immature cells. To identify the PSF1-binding protein, we ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kong L,Ueno M,Itoh M,Yoshioka K,Takakura N

    更新日期:2006-01-27 00:00:00

  • Structural basis for the selective addition of an oxygen atom to cyclic ketones by Baeyer-Villiger monooxygenase from Parvibaculum lavamentivorans.

    abstract::Baeyer-Villiger monooxygenase (BVMO) catalyzes insertion of an oxygen atom into aliphatic or cyclic ketones with high regioselectivity. The BVMOs from Parvibaculum lavamentivorans (BVMOParvi) and Oceanicola batsensis (BVMOOcean) are interesting because of their homologies, with >40% sequence identity, and reaction wit...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Nguyen TD,Choi GE,Gu DH,Seo PW,Kim JW,Park JB,Kim JS

    更新日期:2019-05-07 00:00:00

  • Suppressed hepatocyte proliferation via a ROS-HNE-P21 pathway is associated with nicotine- and cotinine-enhanced alcoholic fatty liver in mice.

    abstract::CYP2A5 is a major enzyme responsible for nicotine and cotinine metabolism in mice. Nicotine and cotinine enhance alcoholic fatty liver in wild type (WT) mice but not in CYP2A5 knockout (KO) mice, and reactive oxygen species (ROS) generated during the CYP2A5-mediated metabolism contributes to the enhancing effect. In c...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chen X,Wang K,Cederbaum AI,Lu Y

    更新日期:2019-04-23 00:00:00

  • Crystal structure of GTPase-activating domain from human MgcRacGAP.

    abstract::Cytokinesis in animal cells relies on a centralspindlin complex consisting of male germ cell RacGap (MgcRacGAP) and mitotic kinesin-like protein 1 (MKLP1). Rho GTPases act as molecular switches to regulate the actin cytoskeleton for cytokinesis, of which Rac1 is regulated by MgcRacGAP. In this study, we determined the...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Matsuura A,Lee HH

    更新日期:2013-06-07 00:00:00

  • α-Synuclein accumulation reduces GABAergic inhibitory transmission in a model of multiple system atrophy.

    abstract::Multiple system atrophy is a neurodegenerative disease caused by abnormal α-synuclein (α-syn) accumulation in oligodendrocytes and neurons. We previously demonstrated that transgenic (Tg) mice that selectively overexpressed human α-syn in oligodendrocytes exhibited neuronal α-syn accumulation. Microtubule β-III tubuli...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ito H,Nakayama K,Jin C,Suzuki Y,Yazawa I

    更新日期:2012-11-23 00:00:00

  • Decrease in cytosolic free Ca2+ and enhanced proteoglycan synthesis induced by cartilage derived growth factors in cultured chondrocytes.

    abstract::Cartilage-derived growth factors, enhance proteoglycan synthesis in cultured chick-embryo chondrocytes, and have almost no effect on cell proliferation. Addition of cartilage derived growth factors to cartilage cells loaded with the fluorescent Ca2+ indicator quin 2, caused a rapid, concentration dependent decrease in...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Eilam Y,Beit-Or A,Nevo Z

    更新日期:1985-10-30 00:00:00

  • A novel and potent ribonuclease from fruiting bodies of the mushroom Pleurotus pulmonarius.

    abstract::A ribonuclease (RNase), with an N-terminal sequence different from those of ribonucleases from the mushrooms Irpex lacteus, Lentinus edodes, Pleurotus ostreatus, Pleurotus tuber-regium, and Volvariella volvacea, was purified from fruiting bodies of the edible mushroom Pleurotus pulmonarius. The N-terminal sequence of ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ye XY,Ng TB

    更新日期:2002-05-03 00:00:00

  • The presence of a side population and its marker ABCG2 in human deciduous dental pulp cells.

    abstract::Stem cells have been identified using the DNA-binding dye Hoechst 33342 and flow cytometry (FCM) in various tissues known as the side population (SP). The present study shows, for the first time, the presence of side population cells in human deciduous dental pulp cells (DPCs). Flow cytometric identification revealed ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wang J,Wei X,Ling J,Huang Y,Huo Y,Zhou Y

    更新日期:2010-09-24 00:00:00

  • Calmodulin regulation of cholinergic muscarinic receptor: effects of calcium and phosphorylating states.

    abstract::Calmodulin (CaM) regulation of cholinergic muscarinic receptor was investigated using synaptic membrane isolated from rat brains and [3H]-QNB as a binding ligand. CaM exerts a biphasic effect on receptor binding showing both a Ca2+-dependent receptor loss and an increase depending on the state of membrane phosphorylat...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ho AK,Wang JH

    更新日期:1985-12-31 00:00:00

  • PICOT is a molecule which binds to anamorsin.

    abstract::Anamorsin (AM) (also called CIAPIN-1) is a cell-death-defying factor. AM deficient mice die during late gestation; AM deficient embryos are anemic and very small compared to wild type (WT) embryos. It is thought that AM plays crucial roles in hematopoiesis and embryogenesis. To clarify the mechanisms of AM functions, ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Saito Y,Shibayama H,Tanaka H,Tanimura A,Matsumura I,Kanakura Y

    更新日期:2011-05-06 00:00:00

  • The N-terminal sequence of antileukoprotease isolated from bronchial secretion.

    abstract::Antileukoprotease (ALP) was isolated from bronchial secretion. The amino acid composition as well as the N-terminal sequence were determined. No homology with other low molecular weight proteinase inhibitors was found. ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Klasen EC,Kramps JA

    更新日期:1985-04-16 00:00:00

  • Cardioprotection by modulation of mitochondrial respiration during ischemia-reperfusion: role of apoptosis-inducing factor.

    abstract::The transient, reversible blockade of electron transport (BET) during ischemia or at the onset of reperfusion protects mitochondria and decreases cardiac injury. Apoptosis inducing factor (AIF) is located within the mitochondrial intermembrane space. A release of AIF from mitochondria into cytosol and nucleus triggers...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Xu A,Szczepanek K,Hu Y,Lesnefsky EJ,Chen Q

    更新日期:2013-06-14 00:00:00

  • Glucocorticoid suppresses the canonical Wnt signal in cultured human osteoblasts.

    abstract::To explore the mechanism of glucocorticoid-induced osteoporosis, we investigated the effect of glucocorticoid on canonical Wnt signaling that emerged as a novel key pathway for promoting bone formation. Wnt3a increased the T-cell factor (Tcf)/lymphoid enhancer factor (Lef)-dependent transcriptional activity in primary...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ohnaka K,Tanabe M,Kawate H,Nawata H,Takayanagi R

    更新日期:2005-04-01 00:00:00

  • Autophagy-dependent mitochondrial function regulates osteoclast differentiation and maturation.

    abstract::Bone homeostasis is maintained by bone remodeling, which involves continuous bone resorption by osteoclasts and bone formation by osteoblasts. Dysregulation of bone turnover, caused by osteoclast overactivation, causes destructive bone diseases. However, the mechanisms underlying the maintenance of osteoclast differen...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Aoki S,Shimizu K,Ito K

    更新日期:2020-07-05 00:00:00

  • Interferon-alpha promotes survival of human primary B-lymphocytes via phosphatidylinositol 3-kinase.

    abstract::Signaling pathways for the antiviral and antiproliferative biological effects of type I interferons (IFN) are well established. In this report we demonstrate a novel signaling pathway for IFN-alpha, as it induced rapid phosphorylation of both PKB/Akt and its substrate forkhead. The PI3-kinase inhibitor LY294002 abolis...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ruuth K,Carlsson L,Hallberg B,Lundgren E

    更新日期:2001-06-15 00:00:00

  • Molecular cloning and sequencing of the murine gastrin gene.

    abstract::Gastrin is a peptide hormone important in acid regulation, growth of enterochromaffin cells of the oxyntic mucosa, and smooth muscle contractility. We isolated a genomic clone of gastrin from a SV-129 murine genomic library. The murine gastrin gene contains an open reading frame of 101 amino acids. The deduced amino a...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Koh TJ,Wang TC

    更新日期:1995-11-02 00:00:00

  • Estrogen effects in the myocardium: inhibition of NF-kappaB DNA binding by estrogen receptor-alpha and -beta.

    abstract::We have previously shown that estrogen effects in the heart include direct hormone effects on the myocardium. In a recent study we found that one beneficial effect of estradiol on the myocardium is the inhibition of apoptosis in cardiac myocytes. This effect was associated with a reduction of NF-kappaB activity. In th...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Pelzer T,Neumann M,de Jager T,Jazbutyte V,Neyses L

    更新日期:2001-09-07 00:00:00

  • Effect of ionomycin on cell pH in isolated renal proximal tubules.

    abstract::In isolated rabbit proximal tubules the addition of 2.0 microM but not 0.2 microM ionomycin induced a sustained increase in cell pH ([pH]i). This [pH]i response to 2.0 microM ionomycin was shown to be independent of several transporters such as Na+/H+ exchanger, Na(+)-HCO3- cotransporter, Cl-/HCO3- exchanger, or H(+)-...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yamada H,Seki G,Taniguchi S,Uwatoko S,Suzuki K,Kurokawa K

    更新日期:1996-08-05 00:00:00

  • Enhanced p73 expression during differentiation and complex p73 isoforms in myeloid leukemia.

    abstract::The p53 homologue p73 is expressed in at least six different isoforms (alpha, beta, gamma, delta, epsilon, and zeta), but unlike p53 it has rarely been found mutated in human cancers. However, altered expression of this gene has been reported in cancer cells. In order to understand if p73 is involved in normal and mal...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Tschan MP,Grob TJ,Peters UR,Laurenzi VD,Huegli B,Kreuzer KA,Schmidt CA,Melino G,Fey MF,Tobler A,Cajot JF

    更新日期:2000-10-14 00:00:00

  • HMGB1-containing nucleosome mediates chemotherapy-induced metastasis of human lung cancer.

    abstract::While chemotherapy is an important and widely used therapeutic for cancer, it may facilitate cancer metastasis. Herein, we report that human lung cancer cells exert higher invasion and metastasis after chemotherapy. In a human lung cancer xenograft model, chemotherapy promotes the cancer invasion and metastasis in HMG...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wang K,Shan S,Wang S,Gu X,Zhou X,Ren T

    更新日期:2018-06-07 00:00:00

  • Overexpression of BCL2 blocks TNF-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in human lung cancer cells.

    abstract::The tumor necrosis factor (TNF) related apoptosis-inducing ligand (TRAIL or Apo2L) and its receptors are members of the tumor necrosis factor superfamily. TRAIL triggers apoptosis by binding to its two proapoptotic receptors DR4 and DR5, a process which is negatively regulated by binding of TRAIL to its two decoy rece...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sun SY,Yue P,Zhou JY,Wang Y,Choi Kim HR,Lotan R,Wu GS

    更新日期:2001-01-26 00:00:00

  • Calcium dependent inositol trisphosphate-induced calcium release in the guinea-pig taenia caeci.

    abstract::The properties of the Ca stores in the skinned fiber bundles of the guinea-pig taenia caeci were studied by a novel method to measure Ca released from the stores using a fluorescent Ca indicator, fura-2. It is found that the capacity of the Ca stores amounts some 170 mumoles/liter cell water. There are at least two Ca...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Iino M

    更新日期:1987-01-15 00:00:00

  • Effects of folic acid deficiency and MTHFRC677T polymorphisms on cytotoxicity in human peripheral blood lymphocytes.

    abstract::Apoptosis (APO) and necrosis (NEC) are two different types of cell death occurring in response to cellular stress factors. Cells with DNA damage may undergo APO or NEC. Folate is an essential micronutrient associated with DNA synthesis, repair and methylation. Methylenetetrahydrofolate reductase (MTHFR) regulates intr...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wu X,Liang Z,Zou T,Wang X

    更新日期:2009-02-13 00:00:00

  • Molecular cloning of the gene for p85 that regulates the initiation of cytokinesis in Tetrahymena.

    abstract::Tetrahymena p85 is localized to the presumptive division plane before division furrow formation; its molecular weight in SDS-polyacrylamide gel electrophoresis differs in wild-type and temperature-sensitive cell-division-arrest mutant cdaA1 cells. At the restrictive temperature, p85 localization and division furrow fo...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Gonda K,Nishibori K,Ohba H,Watanabe A,Numata O

    更新日期:1999-10-14 00:00:00

  • Comprehensive investigation of aberrant microRNAs expression in cells culture model of MnCl2-induced neurodegenerative disease.

    abstract::Manganese (Mn) is required in various human physiological processes. Excessive Mn exposure causes manganism, a progressive neurodegenerative disorder similar to idiopathic Parkinson's disease (IPD). However, the detailed mechanism of Mn-induced neurotoxicity is not yet fully understood. MicroRNAs (miRNAs) play importa...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: He R,Xie X,Lv L,Huang Y,Xia X,Chen X,Zhang L

    更新日期:2017-04-29 00:00:00

  • Synthesis and initial characterization of gamma-L-glutamyl-L-thiothreonylglycine and gamma-L-glutamyl-L-allo-thiothreonylglycine as steric probes of the active site of glyoxalase I.

    abstract::The diastereomeric GSH derivatives gamma-L-Glu-L-allo-thioThr-Gly (6) and gamma-L-Glu-L-thioThr-Gly (6a) have been synthesized as specific probes of the steric environment near the cysteinyl residue of enzyme bound glutathionyl substrates. Experiments with glyoxalase I indicate that while 6a-methylglyoxal thiohemiacet...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Xie XF,Creighton DJ

    更新日期:1991-05-31 00:00:00

  • DNA-binding specificity of the Lon protease alpha-domain from Brevibacillus thermoruber WR-249.

    abstract::Lon protease has been well studied in many aspects; however, the DNA-binding specificity of Lon in prokaryotes has not been clearly identified. Here we examined the DNA-binding activity of Lon protease alpha-domains from Brevibacillus thermoruber (Bt), Bacillus subtilis (Bs), and Escherichia coli (Ec). MALDI-TOF mass ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lin YC,Lee HC,Wang I,Hsu CH,Liao JH,Lee AY,Chen C,Wu SH

    更新日期:2009-10-09 00:00:00