Abstract:
:Characterization of functional vessels is required either for monitoring hemodynamics or patterning of functional vasculature in experimental models. Haemoglobin (Hb) staining is a traditionally used approach for determining the differentiation of erythroid cells. In this investigation, we tested if HB staining can be used for portraying of functional vasculature in experimental zebrafish embryos. The staining sufficiently revealed aortic arches, dorsal aorta, posterior cardinal vein, dorsal longitudinal anastomotic vessels, intersegmental vessels as well as subintestinal vessel basket. We conclude that Hb staining offers an informative and rapid method for in vivo portraying of functional vasculature in experimental zebrafish embryos. It is also suitable for large scale experiments.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Zheng PP,Severijnen LA,Willemsen R,Kros JMdoi
10.1016/j.bbrc.2009.01.175subject
Has Abstractpub_date
2009-03-20 00:00:00pages
823-4issue
4eissn
0006-291Xissn
1090-2104pii
S0006-291X(09)00223-Xjournal_volume
380pub_type
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