当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Crystal structure of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, an NAD⁺-dependent dismutase from Mesorhizobium loti.

Crystal structure of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, an NAD⁺-dependent dismutase from Mesorhizobium loti.

Abstract：

:5-Formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase (FHMPCDH) from Mesorhizobium loti is the fifth enzyme in degradation pathway I for pyridoxine. The enzyme catalyzes a dismutation reaction: the oxidation of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid (FHMPC) to 3-hydroxy-2-methylpyridine 4,5-dicarboxylic acid with NAD(+) and reduction of FHMPC to 4-pyridoxic acid with NADH. FHMPCDH belongs to the l-3-hydroxyacyl-CoA dehydrogenase (HAD) family. The crystal structure was determined by molecular replacement and refined to a resolution of 1.55Å (R-factor of 16.4%, Rfree=19.4%). There were two monomers in the asymmetric unit. The overall structure of the monomer consisted of N- and C-terminal domains connected by a short linker loop. The monomer was similar to members of the HAD family (RMSD=1.9Å). The active site was located between the domains and highly conserved to that of human heart l-3-hydroxyacyl-CoA dehydrogenase (HhHAD). His-Glu catalytic dyad, a serine and two asparagine residues of HhHAD were conserved. Ser116, His137 and Glu149 in FHMPCDH are connected by a hydrogen bonding network forming a catalytic triad. The functions of the active site residues in the reaction mechanism are discussed.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Mugo AN,Kobayashi J,Mikami B,Yoshikane Y,Yagi T,Ohnishi K

doi

10.1016/j.bbrc.2014.11.028

subject

Has Abstract

pub_date

2015-01-02 00:00:00

pages

35-40

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(14)02038-5

journal_volume

456

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Characterization of the mouse Myoc/Tigr gene.

    abstract：:Mutations in the myocilin (MYOC), also known as Trabecular meshwork-Inducible Glucocorticoid Response (TIGR) gene can lead to juvenile open-angle glaucoma in human and may be responsible for at least 3% of primary open-angle glaucoma. To develop a mouse model of primary open angle glaucoma, and to get deeper insight i...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8541

    authors： Tomarev SI,Tamm ER,Chang B

    更新日期：1998-04-28 00:00:00

  • Crystal structure and biophysical characterisation of Helicobacter pylori phosphopantetheine adenylyltransferase.

    abstract：:Helicobacter pylori is a bacterium that causes chronic active gastritis and peptic ulcers. Drugs targeting H. pylori phosphopantetheine adenylyltransferase (HpPPAT), which is involved in CoA biosynthesis, may be useful. Herein, we report the expression in Escherichia coli and purification of recombinant HpPPAT and des...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.04.058

    authors： Cheng CS,Chen CH,Luo YC,Chen WT,Chang SY,Lyu PC,Kao MC,Yin HS

    更新日期：2011-05-06 00:00:00

  • The homology of complement factor C8 gamma chain and alpha-1-microglobulin.

    abstract：:The sequence of the complement factor C8 gamma chain shares a remarkable degree of similarity with that of alpha-1-microglobulin, a member of the alpha-2u-globulin superfamily. This superfamily comprises a diverse group of distantly related animal proteins possessing characteristic structural features and similar func...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)91636-6

    authors： Hunt LT,Elzanowski A,Barker WC

    更新日期：1987-11-30 00:00:00

  • Presynaptic GABA(B) receptors modulate synaptic facilitation and depression at distinct synapses in fusiform cells of mouse dorsal cochlear nucleus.

    abstract：:The mammalian dorsal cochlear nucleus (DCN) is considered to contribute to the localization of the sound sources. Fusiform cells (FCs), principal projection neurons in the DCN, integrate two excitatory inputs from auditory nerve fibers (ANFs) and parallel fibers (PFs). Although an immunohistochemical study suggested p...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.01.001

    authors： Irie T,Ohmori H

    更新日期：2008-03-07 00:00:00

  • Sirt3 binds to and deacetylates mitochondrial pyruvate carrier 1 to enhance its activity.

    abstract：:Mitochondrial pyruvate carrier (MPC), composed of MPC1 and MPC2, can modulate pyruvate oxidation in mitochondrial and MPC1 expression correlates with poor prognosis of multiple cancers. Here, we reported that MPC1 is acetylated and its main acetylation sites are: K45 and K46. Sirt3 binds to and deacetylates MPC1. High...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.11.036

    authors： Liang L,Li Q,Huang L,Li D,Li X

    更新日期：2015-12-25 00:00:00

  • Uniaxial cyclic stretch induces focal adhesion kinase (FAK) tyrosine phosphorylation followed by mitogen-activated protein kinase (MAPK) activation.

    abstract：:We investigated the role of tyrosine phosphorylation of FAK in the stretch-induced MAPKs (extracellular signal-regulated kinase (ERK), p38MAPK) activation in mutant FAK-transfected fibroblasts. In response to uniaxial cyclic stretch (1 Hz, 120% in length), the levels of tyrosine phosphorylation of the Tyr-397 and Tyr-...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5775

    authors： Wang JG,Miyazu M,Matsushita E,Sokabe M,Naruse K

    更新日期：2001-10-26 00:00:00

  • Reducing the effects of intracellular accumulation of thermolabile collagen II mutants by increasing their thermostability in cell culture conditions.

    abstract：:Mutations in collagen II are associated with spondyloepiphyseal dysplasia, a group of heritable diseases whose common features include aberrations of skeletal growth. The mechanisms through which mutations in collagen II affect the cartilaginous tissues are complex and include both intracellular and extracellular proc...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.04.056

    authors： Gawron K,Jensen DA,Steplewski A,Fertala A

    更新日期：2010-05-28 00:00:00

  • NO-donating aspirin inhibits intestinal carcinogenesis in Min (APC(Min/+)) mice.

    abstract：:The chemopreventive effect of nitric oxide-releasing aspirin (NO-ASA) against gastrointestinal tumorigenesis was evaluated in Min (APC(Min/+)) mice. NO-ASA consists of a traditional ASA that bears covalently attached to it an NO-releasing moiety. Four groups (N=10) of six-week-old female C57BL/6J APC(Min/+) and the co...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.12.015

    authors： Williams JL,Kashfi K,Ouyang N,del Soldato P,Kopelovich L,Rigas B

    更新日期：2004-01-16 00:00:00

  • A regulatory role of Wnt signaling pathway in the hematopoietic differentiation of murine embryonic stem cells.

    abstract：:One of the most important issues in stem cell research is to understand the regulatory mechanisms responsible for their differentiation. An extensive understanding of mechanism underlying the process of differentiation is crucial in order to prompt stem cells to perform a particular function after differentiation. To ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.09.206

    authors： Feng Z,Srivastava AS,Mishra R,Carrier E

    更新日期：2004-11-26 00:00:00

  • Generation of cloned calves and transgenic chimeric embryos from bovine embryonic stem-like cells.

    abstract：:Bovine embryonic stem-like cells (ES-like cells) appear to maintain a normal diploid karyotype indefinitely during culture in vitro and to express marker proteins that are characteristic of ES cells from mice, namely, alkaline phosphatase (AP), stage-specific embryonic antigen-1 (SSEA-1), STAT-3, and Oct 4. After prol...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)01536-5

    authors： Saito S,Sawai K,Ugai H,Moriyasu S,Minamihashi A,Yamamoto Y,Hirayama H,Kageyama S,Pan J,Murata T,Kobayashi Y,Obata Y,Yokoyama KK

    更新日期：2003-09-12 00:00:00

  • Histone acetyltransferase MOF is involved in suppression of endometrial cancer and maintenance of ERα stability.

    abstract：:Histone acetyltransferase MOF is involved in active transcription regulation through histone H4K16 acetylation. MOF is downexpressed in a number of human tumors, but biological function of MOF in endometrial cancer has not been fully defined. The estrogen receptor α (ERα) is a transcription factor that regulates estro...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.10.090

    authors： Wu Y,Zeng K,Wang C,Wang S,Sun H,Liu W,Wang X,Niu J,Cong SY,Zhou X,Zhao Y

    更新日期：2019-02-05 00:00:00

  • Phospholipase C δ1 negatively regulates autophagy in colorectal cancer cells.

    abstract：:Colorectal cancer (CRC) is one of the leading causes of cancer-related death worldwide. Kirsten rat sarcoma viral oncogene homolog (KRAS) is frequently mutated in CRC, and KRAS mutations promote cell motility, growth, and survival. We previously revealed that the expression of phospholipase C (PLC) δ1, one of the most...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.05.098

    authors： Shimozawa M,Anzai S,Satow R,Fukami K

    更新日期：2017-07-08 00:00:00

  • 1,25-Dihydroxycholecalciferol enhances butyrate-induced p21(Waf1/Cip1) expression.

    abstract：:Butyrate, a short-chain fatty acid produced in the colon, as well as its prodrug tributyrin, reduce proliferation and increase differentiation of colon cancer cells. p21(Waf1/Cip1) and p27(Kip1) are negative regulators of cell cycle and are thought to have a key function in the differentiation of various cell lines. W...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.4756

    authors： Gaschott T,Wächtershäuser A,Steinhilber D,Stein J

    更新日期：2001-04-27 00:00:00

  • Distinct distributions of mu, delta and kappa opioid receptor mRNA in rat brain.

    abstract：:We present a comprehensive comparison of the anatomical distributions of the cloned mu, delta and kappa opioid receptor mRNA in rat brain. Northern blot analysis revealed that mRNA species encoding the three receptors differed in size and were differentially localized in brain regions. In peripheral tissues analyzed, ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2826

    authors： George SR,Zastawny RL,Briones-Urbina R,Cheng R,Nguyen T,Heiber M,Kouvelas A,Chan AS,O'Dowd BF

    更新日期：1994-12-15 00:00:00

  • Tubby domain superfamily protein is required for the formation of the 7S SNARE complex in Drosophila.

    abstract：:Tubby domain superfamily protein (TUSP) is a distant member of the Tubby-like protein (TULP) family. Although other TULPs play important roles in sensation, metabolism, and development, the molecular functions of TUSP are completely unknown. Here, we explore the function of TUSP in the Drosophila nervous system where ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.11.117

    authors： Yoon EJ,Jeong YT,Lee JE,Moon SJ,Kim CH

    更新日期：2017-01-22 00:00:00

  • Multiple processing forms and their biological activities of a novel angiogenesis inhibitor vasohibin.

    abstract：:Vasohibin is a newly identified negative feedback regulator for angiogenesis. When expressed in cultured human endothelial cells, vasohibin polypeptides were detected in multiple distinct molecular weight forms, suggesting that some proteolytic events may occur within cells or the pericellular milieu. In order to iden...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.01.185

    authors： Sonoda H,Ohta H,Watanabe K,Yamashita H,Kimura H,Sato Y

    更新日期：2006-04-07 00:00:00

  • Conversion of desulforedoxin into a rubredoxin center.

    abstract：:Rubredoxin and desulforedoxin both contain an Fe(S-Cys)4 center. However, the spectroscopic properties of the center in desulforedoxin differ from rubredoxin. These differences arise from a distortion of the metal site hypothesized to result from adjacent cysteine residues in the primary sequence of desulforedoxin. Tw...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6171

    authors： Yu L,Kennedy M,Czaja C,Tavares P,Moura JJ,Moura I,Rusnak F

    更新日期：1997-02-24 00:00:00

  • Covalent conjugation of Groucho with SUMO-1 modulates its corepressor activity.

    abstract：:Groucho is a corepressor that forms a macromolecular complex for its corepressor activity, in which HDAC1 is an essential component for the modulation of chromatin structure and transcriptional repression of target genes. Here, we show that Groucho is covalently conjugated with small ubiquitin-related modifier-1 (SUMO...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.12.043

    authors： Ahn JW,Lee YA,Ahn JH,Choi CY

    更新日期：2009-01-30 00:00:00

  • Ectopic expression of the CDK inhibitor p21Cip1 enhances deregulated E2F activity and increases cancer cell-specific cytotoxic gene expression mediated by the ARF tumor suppressor promoter.

    abstract：:In cancer treatment, specifically targeting cancer cells is important for optimal therapeutic efficacy. One strategy is to utilize a cancer specific promoter to express a cytotoxic gene or a viral gene required for replication. In this approach, the therapeutic window is dependent on the relative promoter activity in ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.12.185

    authors： Kurayoshi K,Shiromoto A,Ozono E,Iwanaga R,Bradford AP,Araki K,Ohtani K

    更新日期：2017-01-29 00:00:00

  • Opioids inhibit the induction of nitric oxide synthase in J774 macrophages.

    abstract：:The effect of opioids on NO production by LPS-stimulated murine macrophages J774 has been investigated. Morphine (mu and k opioid receptor agonist), DAGO (selective mu receptor agonist) and U50-488H (selective k receptor agonist), added (10(-10)-10(-6) M) to the cells 0.5 h before activation with LPS, significantly in...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2065

    authors： Iuvone T,Capasso A,D'Acquisto F,Carnuccio R

    更新日期：1995-07-26 00:00:00

  • Identification of ligand binding residues in extracellular loops of the melanocortin 1 receptor.

    abstract：:To investigate whether residues in the extracellular domains of melanocortin 1 receptor (MC1R) are required for ligand binding, a number of mutants were constructed where charged residues were converted to alanine. The residues targeted for mutagenesis were Ser6, Glu102, Arg109, Asp184, Glu269, and Thr272. The mutant ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0266

    authors： Chhajlani V,Xu X,Blauw J,Sudarshi S

    更新日期：1996-02-15 00:00:00

  • Protein arginine methyltransferase 1 mediates regeneration in Dugesia japonica.

    abstract：:As a typical organism of platyhelminth, Dugesia japonica attracts more and more attention for its strong regenerative ability. Protein arginine methyltransferase (PRMT) family is composed of a class of enzymes with methyltransferase activities, which play fundamental roles in vivo in many important physiological proce...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.01.087

    authors： Zhang Y,Wu W,Gao L,Chen M,Liu X,Huang M,Li A,Zheng K,Liu D,Deng H,Zhao B,Liu B,Pang Q

    更新日期：2020-04-02 00:00:00

  • Different roles of GPR120 and GPR40 in the acquisition of malignant properties in pancreatic cancer cells.

    abstract：:Free fatty acids (FFAs) act as extracellular signaling molecules through binding to G-protein-coupled FFA receptors (FFARs). GPR120 and GPR40 are identified as FFARs for medium- and long-chain fatty acids. In the present study, we investigated roles of GPR120 and GPR40 in cellular functions of pancreatic cancer PANC-1...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.08.050

    authors： Fukushima K,Yamasaki E,Ishii S,Tomimatsu A,Takahashi K,Hirane M,Fukushima N,Honoki K,Tsujiuchi T

    更新日期：2015-09-25 00:00:00

  • Hypolipidemic mechanism of oryzanol components- ferulic acid and phytosterols.

    abstract：UNLABELLED:The effect of oryzanol (well known hypolipidemic component in rice bran oil) and its chemical constituents- ferulic acid (FA) and phytosterols on hypolipidemia were investigated. METHODS AND RESULTS:Docking (in silico) studies showed that FA had a better binding ability with lipase while sterols bound well ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.05.053

    authors： Bhaskaragoud G,Rajath S,Mahendra VP,Kumar GS,Gopala Krishna AG,Kumar GS

    更新日期：2016-07-22 00:00:00

  • Measurement of damage-specific DNA incision by nucleotide excision repair in vitro.

    abstract：:We have devised a method to evaluate the capacity of mammalian cell extracts to incise damaged DNA in vitro. The assay uses damaged-plasmid DNA as a substrate for nucleotide excision repair by cell extracts. During this process, enzymatic incision of the damaged DNA is followed by DNA resynthesis. Under our assay cond...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1999

    authors： Calsou P,Salles B

    更新日期：1994-07-29 00:00:00

  • Temperature acclimation induces light meromyosin isoforms with different primary structures in carp fast skeletal muscle.

    abstract：:Carp acclimated to 10 degrees C gave 69k, 66k, and 62kDa light meromyosin (LMM) fragments in SDS-PAGE, while fish acclimated to 30 degrees C gave 74k, 69k, 66k, and 62kDa fragments. The microsequence analysis revealed that the 69k and 66kDa components from the 10 degrees C-acclimated carp contained an N-terminal amino...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1313

    authors： Watabe S,Imai J,Nakaya M,Hirayama Y,Okamoto Y,Masaki H,Uozumi T,Hirono I,Aoki T

    更新日期：1995-03-08 00:00:00

  • Role of sigma factor E in regulation of Salmonella Agf expression.

    abstract：:Expression of thin aggregative fimbriae (Agf) in Salmonella, which is responsible for bacterial cell adhesion to surfaces, aggregation, and formation of biofilms, is regulated by a complex mechanism. In order to identify gene(s) involved in the expression of Agf, the TnphoA transposon was introduced into Salmonella ty...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.11.025

    authors： Yoo AY,Yu JE,Yoo H,Lee TH,Lee WH,Oh JI,Kang HY

    更新日期：2013-01-04 00:00:00

  • Haemoglobin staining for in vivo portraying of functional vasculature in experimental zebrafish embryos.

    abstract：:Characterization of functional vessels is required either for monitoring hemodynamics or patterning of functional vasculature in experimental models. Haemoglobin (Hb) staining is a traditionally used approach for determining the differentiation of erythroid cells. In this investigation, we tested if HB staining can be...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.01.175

    authors： Zheng PP,Severijnen LA,Willemsen R,Kros JM

    更新日期：2009-03-20 00:00:00

  • Mechanism of cytotoxicity of aflatoxin B1: role of cytochrome P1-450.

    abstract：:A mouse hepatoma cell line, Hepa-1, is highly sensitive to the toxic effects of Aflatoxin B1 (AFB1). Half maximal survival (LD50) of cells occurs at 0.068 ug AFB1/ml. Benzo(a)anthracene, which induces aryl hydrocarbon hydroxylase and cytochrome P1-450 in Hepa-1, causes a slight increase in the toxicity of AFB1 (LD50 =...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)91043-6

    authors： Kärenlampi SO

    更新日期：1987-06-15 00:00:00

  • A novel microgravimetric DNA sensor with high sensitivity.

    abstract：:A novel method using an amplifier with a cantilever and gold nanoparticles successfully to extend the length of the target for the specific and high sensitive detection of DNA was reported. When the size of gold nanoparticle is 50 nm, a sensitivity of 10(-15)M for the single base mutation detection has been achieved. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)00531-x

    authors： Liu T,Tang J,Han M,Jiang L

    更新日期：2003-04-25 00:00:00