Abstract:
:The effect of opioids on NO production by LPS-stimulated murine macrophages J774 has been investigated. Morphine (mu and k opioid receptor agonist), DAGO (selective mu receptor agonist) and U50-488H (selective k receptor agonist), added (10(-10)-10(-6) M) to the cells 0.5 h before activation with LPS, significantly inhibited NO production. This effect was reverted by naloxone (10(-12)-10(-8) M), an opioid specific antagonist. In contrast, DPDPE and deltorphin II respectively delta 1 and delta 2 receptor agonists (10(-10)-10(-6) M) did not affect NO generation. Morphine was not able to inhibit NO production when added after LPS challenge. The results of the present study indicate that opioids are able to inhibit NO formation in LPS-activated macrophages through the involvement of specific opioid receptors. Moreover, the ability of morphine to inhibit NO production only when given before LPS challenge suggests that the opiate inhibits the induction but not the activity of the inducible NO synthase.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Iuvone T,Capasso A,D'Acquisto F,Carnuccio Rdoi
10.1006/bbrc.1995.2065subject
Has Abstractpub_date
1995-07-26 00:00:00pages
975-80issue
3eissn
0006-291Xissn
1090-2104pii
S0006291X85720657journal_volume
212pub_type
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