Use of small fluorescent molecules to monitor channel activity.

abstract：:It has been known that Porphyromonas gingivalis has an obligate requirement for hemin or selected heme- or Fe-containing compounds for its growth. In addition, the influence of hemin on the expression of several putative virulence factors produced by this bacterium has also been recently documented; however, the mecha...

journal_title：Biochemical and biophysical research communications

authors： Shibata Y,Hiratsuka K,Hayakawa M,Shiroza T,Takiguchi H,Nagatsuka Y,Abiko Y

更新日期：2003-01-10 00:00:00

abstract：:The role of hyaluronan binding protein 1 (HABP1) in cell signaling was investigated and in vitro kinase assay demonstrated that it is a substrate for MAP kinase. Phosphorylation of endogenous HABP1 was also observed following treatment of J774 cells with PMA. HABP1 was coimmunoprecipitated with activated ERK, confirmi...

journal_title：Biochemical and biophysical research communications

authors： Majumdar M,Meenakshi J,Goswami SK,Datta K

更新日期：2002-03-08 00:00:00

abstract：:In this study evidence for the presence of specific cytosolic platelet-activating factor (PAF) binding sites has been presented. The equilibrium dissociation constant (Kd) as determined by Scatchard analysis was 4.29 x 10(-9) M for the cytosolic and 3.71 x 10(-9) M for the membrane fraction. The maximal number of bind...

journal_title：Biochemical and biophysical research communications

authors： Svetlov S,Nigam S

更新日期：1993-01-15 00:00:00

abstract：:A20 is a zinc finger protein that renders cells resistant to apoptosis. However, the recent demonstration that A20-deficient mice develop severe inflammation and are hyper-responsive to LPS suggests that A20 may play a key role in regulating the inflammatory response. This study, for the first time, explores the likel...

journal_title：Biochemical and biophysical research communications

authors： O'Reilly SM,Moynagh PN

更新日期：2003-04-04 00:00:00

abstract：:The organic anion and cation transporters (OATs and OCTs) are a large family (SLC22) of transmembrane proteins that are able to transport a variety of compounds including drugs, environmental toxins, and endogenous metabolites. OATs are expressed in various tissues, primarily kidney and liver, but also in placenta, sm...

journal_title：Biochemical and biophysical research communications

authors： Monte JC,Nagle MA,Eraly SA,Nigam SK

更新日期：2004-10-15 00:00:00

abstract：:GGAs (Golgi-localizing, gamma-adaptin ear domain homology, ADP-ribosylation factor (ARF)-binding proteins), constitute a family of monomeric adaptor proteins and are associated with protein trafficking from the trans-Golgi network to endosomes. Here, we show that GGA3 is monoubiquitylated by a RING-H2 type-ubiquitin l...

journal_title：Biochemical and biophysical research communications

authors： Yogosawa S,Kawasaki M,Wakatsuki S,Kominami E,Shiba Y,Nakayama K,Kohsaka S,Akazawa C

更新日期：2006-11-10 00:00:00

abstract：:Antithrombin III (ATIII) performs a critical anticoagulant function by precluding the activation of blood clotting proteinases, aided by its two cofactors, heparin and heparan sulfate. Though several studies have been carried out on physiological, biochemical and structural perspectives on ATIII, so far there are limi...

journal_title：Biochemical and biophysical research communications

authors： Kumar A,Bhandari A,Sarde SJ,Goswami C

更新日期：2013-11-01 00:00:00

abstract：:We studied about the physicochemical stability of a novel heparin-binding growth/differentiation factor, midkine (MK). It was found that synthetic human MK was heat and acid stable. Neither incubation at 80 degrees C for 90 sec nor treatment at low pH affected the elution profile of MK molecule on the high performance...

journal_title：Biochemical and biophysical research communications

authors： Kojima S,Inui T,Muramatsu H,Kimura T,Sakakibara S,Muramatsu T

更新日期：1995-11-13 00:00:00

abstract：:Prostate cancer (PCa) is the second leading cause of cancer-associated mortality in men. Speckle-type pox virus and zinc finger protein (SPOP), the most frequently mutated gene in PCa, functions as a tumor suppressor via degradation of cancer-promoting substrates. However, its upstream regulation in PCa metastasis rem...

journal_title：Biochemical and biophysical research communications

authors： Lv W,Huan M,Yang W,Gao Y,Wang K,Xu S,Zhang M,Ma J,Wang X,Chen Y,Li L

更新日期：2020-08-27 00:00:00

abstract：:The alpha(1c) subunit of the cardiac L-type Ca(2+) channel, which contains the channel pore, voltage- and Ca(2+)-dependent gating structures, and drug binding sites, has been well studied in heterologous expression systems, but many aspects of L-type Ca(2+) channel behavior in intact cardiomyocytes remain poorly chara...

journal_title：Biochemical and biophysical research communications

authors： Ganesan AN,O'Rourke B,Maack C,Colecraft H,Sidor A,Johns DC

更新日期：2005-04-08 00:00:00

abstract：:S-adenosyl-L-methionine (SAM)-dependent methyltransferases (MTases) methylate diverse biological molecules using a SAM cofactor. The ytqB gene of Bacillus subtilis encodes a putative MTase and its biological function has never been characterized. To reveal the structural features and the cofactor binding mode of YtqB,...

journal_title：Biochemical and biophysical research communications

authors： Park SC,Song WS,Yoon SI

更新日期：2014-04-18 00:00:00

abstract：:Methanococcus voltae produces two flagellins of molecular weight 31,000 and 33,000. Amino acid analysis as well as peptide mapping with cyanogen bromide, chymotrypsin and Staphylococcus aureus V-8 protease indicates that the two flagellins are distinct. N-terminal sequencing of the 31,000 Mc. voltae flagellin as well ...

journal_title：Biochemical and biophysical research communications

authors： Kalmokoff ML,Karnauchow TM,Jarrell KF

更新日期：1990-02-28 00:00:00

abstract：:Many proteins in bacterial cells fold in the chaperonin cage made of the central cavity of GroEL capped by GroES. Recent studies indicate that the polypeptide in the cage spends the most time as a state tethered dynamically to the GroEL/GroES interface region, in which folding occurs in the polypeptide segments away f...

journal_title：Biochemical and biophysical research communications

authors： Motojima F,Yoshida M

更新日期：2015-10-09 00:00:00

abstract：:The important role of pyruvate kinase during malarial infection has prompted the cloning of a cDNA encoding Plasmodium falciparum pyruvate kinase (pfPyrK), using mRNA from intraerythrocytic-stage malaria parasites. The full-length cDNA encodes a protein with a computed molecular weight of 55.6 kDa and an isoelectric p...

journal_title：Biochemical and biophysical research communications

authors： Chan M,Sim TS

更新日期：2005-01-07 00:00:00

abstract：BACKGROUND & AIMS:Concentrations of the branched-chain amino acid (BCAA) in the serum of patients with liver cirrhosis correlate with their liver function. Oral administration of BCAA can ameliorate hypoalbuminemia and hepatic encephalopathy. In this study, we aim to clarify the role of BCAA in regulating the replicati...

journal_title：Biochemical and biophysical research communications

authors： Ishida H,Kato T,Takehana K,Tatsumi T,Hosui A,Nawa T,Kodama T,Shimizu S,Hikita H,Hiramatsu N,Kanto T,Hayashi N,Takehara T

更新日期：2013-07-19 00:00:00

abstract：:Incubation of purified protein kinase C with H2O2 results in the generation of a persistently activated form of the enzyme which is no longer dependent on Ca2+ or lipid cofactors. This oxidative activation of purified protein kinase C requires added Fe2+ in the incubation medium. Treatment of the soluble fraction of h...

journal_title：Biochemical and biophysical research communications

authors： Palumbo EJ,Sweatt JD,Chen SJ,Klann E

更新日期：1992-09-30 00:00:00

abstract：:Heparin-binding EGF-like growth factor (HB-EGF) is a polypeptide with an apparent molecular weight of 22 kilodalton that is related to epidermal growth factor (EGF) and that binds and activates the EGF receptor. We examined HB-EGF biological action and expression in human pancreatic cancer cell lines, and compared HB-...

journal_title：Biochemical and biophysical research communications

authors： Kobrin MS,Funatomi H,Friess H,Buchler MW,Stathis P,Korc M

更新日期：1994-08-15 00:00:00

abstract：:Voltage-gated sodium channels (Na(v)) consist of a pore-forming alpha subunit (Na(v)alpha) associated with beta regulatory subunits (Na(v)beta). Adult skeletal myocytes primarily express Na(v)1.4 channels. We found, however, using neonatal L6E9 myocytes, that myofibers acquire a Na(v)1.5-cardiac-like phenotype efficie...

journal_title：Biochemical and biophysical research communications

authors： David M,Martínez-Mármol R,Gonzalez T,Felipe A,Valenzuela C

更新日期：2008-04-11 00:00:00

abstract：:Mouse thymocytes were separated into cortical and medullary subpopulations by differential agglutination with peanut agglutinin. A high-affinity receptor for 1,25-dihydroxyvitamin D3 is present in medullary immunocompetent mouse thymocytes and is absent from cortical immature cells. 1,25-dihydroxyvitamin D3, at physio...

journal_title：Biochemical and biophysical research communications

authors： Ravid A,Koren R,Novogrodsky A,Liberman UA

更新日期：1984-08-30 00:00:00

abstract：:Intravenous immunoglobulin (IVIG) is periodically administered to immunocompromised patients together with antimicrobial agents. The evidence that supports the effectiveness of IVIG is mostly based on data from randomized clinical trials; the underlying mechanisms are poorly understood. A recent study revealed that ki...

journal_title：Biochemical and biophysical research communications

authors： Matsuo H,Itoh H,Kitamura N,Kamikubo Y,Higuchi T,Shiga S,Ichiyama S,Kondo T,Takaori-Kondo A,Adachi S

更新日期：2015-08-14 00:00:00

abstract：:Lipid rafts often serve as an entry site for certain viruses. Here, we report that lipid rafts in Vero E6 cells are involved in the entry of severe acute respiratory syndrome coronavirus (SARS-CoV). Infectivity assay showed the integrity of lipid rafts was required for productive infection of pseudotyped SARS-CoV. Dep...

journal_title：Biochemical and biophysical research communications

authors： Lu Y,Liu DX,Tam JP

更新日期：2008-05-02 00:00:00

abstract：:The interaction between isolated adrenal medullary plasma membranes and chromaffin granules has been proposed as a cell-free model for exocytosis. Phosphorylation experiments showed that isolated chromaffin granules as well as isolated plasma membranes contain protein kinases and phosphate accepting membranous protein...

journal_title：Biochemical and biophysical research communications

authors： Konings F,De Potter W

更新日期：1983-01-14 00:00:00

abstract：:Cultured human lymphocytes and fibroblasts accumulate methotrexate during 24 hours and synthesize methotrexate polyglutamates up to the hexaglutamate, with the triglutamate predominating. In the interval after incubation with methotrexate, drug is lost, metabolites are converted to longer chain-lengths, and methotrexa...

journal_title：Biochemical and biophysical research communications

authors： Whitehead VM,Kalman TI,Vuchich MJ

更新日期：1987-04-14 00:00:00

abstract：:Baeyer-Villiger monooxygenase (BVMO) catalyzes insertion of an oxygen atom into aliphatic or cyclic ketones with high regioselectivity. The BVMOs from Parvibaculum lavamentivorans (BVMOParvi) and Oceanicola batsensis (BVMOOcean) are interesting because of their homologies, with >40% sequence identity, and reaction wit...

journal_title：Biochemical and biophysical research communications

authors： Nguyen TD,Choi GE,Gu DH,Seo PW,Kim JW,Park JB,Kim JS

更新日期：2019-05-07 00:00:00

abstract：:CYP153 a cytochrome P450 from Acinetobacter sp. EB104 catalyzes the hydroxylation of unsubstituted n-alkanes. We have decided to use the CYP153 system as a model for mechanistic studies on regioselective n-alkane oxidation and the interaction of hydrophobic substrates with soluble enzymes. Here the molecular cloning o...

journal_title：Biochemical and biophysical research communications

authors： Maier T,Förster HH,Asperger O,Hahn U

更新日期：2001-08-24 00:00:00

abstract：:Acyl-CoA:cholesterol acyltransferase (ACAT) plays important roles in cellular cholesterol homeostasis. Two ACAT genes exist in mammals. We report here the genomic organization of human ACAT-2 gene and analysis of its promoter activity in various cell lines. The human ACAT-2 gene spans over 18 kb and contains 15 exons....

journal_title：Biochemical and biophysical research communications

authors： Song BL,Qi W,Yang XY,Chang CC,Zhu JQ,Chang TY,Li BL

更新日期：2001-03-30 00:00:00

abstract：:7,8-Dihydrobiopterin is not an intermediate in the de novo biosynthesis of tetrahydrobiopterin, the cofactor required for aromatic amino acid hydroxylations. However, N-acetyl-serotonin inhibition of sepiapterin reductase, an enzyme whose previously only known function was the reduction of sepiapterin to 7,8-dihydrobi...

journal_title：Biochemical and biophysical research communications

authors： Milstien S,Kaufman S

更新日期：1983-09-30 00:00:00

abstract：:IL-10 producing B (B10) cells, a subset of regulatory B (Breg) cells, produce IL-10 and play immunosuppressive roles in antitumor immunity. B10 cells are associated with enhanced tumor-aggressiveness and a poorer prognosis. To specifically inhibit the IL-10 secreted by B cells, we constructed the recombinant plasmid p...

journal_title：Biochemical and biophysical research communications

authors： Zhao R,Liu M,Li X,Chen H,Deng J,Huang C,Zhang XL,Pan Q

更新日期：2018-12-02 00:00:00

abstract：:We found new photochemical intermediate of third rhodopsin-like pigment (tR) or slow cycling rhodopsin-like pigment (sR) in Halobacterium halobium, which was produced by simultaneous illumination with red and blue light. This illumination is employed for measurements of negative phototaxis. The formation of this inter...

journal_title：Biochemical and biophysical research communications

authors： Tomioka H,Kamo N,Takahashi T,Kobatake Y

更新日期：1984-09-28 00:00:00

abstract：:The structural changes of the F-actin-myosin head (S1) complex during the cross-bridge cycle are essential in muscle contraction. Although a large body of evidence has accumulated showing that the actin: S1 stoichiometry in the decorated F-actin-S1 filament is 1:1 at saturation by S1, a recent report by Andreev and Bo...

journal_title：Biochemical and biophysical research communications

authors： Carlier MF,Didry D

更新日期：1992-03-31 00:00:00