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A proton-activated, outwardly rectifying chloride channel in human umbilical vein endothelial cells.

Abstract：

:Extracellular acidic pH-activated chloride channel I(Cl, acid), has been characterized in HEK 293 cells and mammalian cardiac myocytes. This study was designed to characterize I(Cl,acid) in human umbilical vein endothelial cells(HUVECs). The activation and deactivation of the current rapidly and repeatedly follows the change of the extracellular solution at pH 4.3, with the threshold pH 5.3. In addition, at very positive potentials, the current displays a time-dependent facilitation. pH-response relationship for I(Cl,acid) revealed that EC(50) is pH 4.764 with a threshold pH value of pH 5.3 and nH of 14.545. The current can be blocked by the Cl(-) channel inhibitor DIDS (100 microM). In summary, for the first time we report the presence of proton-activated, outwardly rectifying chloride channel in HUVECs. Because an acidic environment can develop in local myocardium under pathological conditions such as myocardial ischemia, I(Cl,acid) would play a role in regulation of EC function under these pathological conditions.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Ma ZY,Zhang W,Chen L,Wang R,Kan XH,Sun GZ,Liu CX,Li L,Zhang Y

doi

10.1016/j.bbrc.2008.04.090

subject

Has Abstract

pub_date

2008-07-04 00:00:00

pages

437-40

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(08)00774-2

journal_volume

371

pub_type

杂志文章

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