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Isolation of laminin from human placental basement membranes: amnion, chorion and chorionic microvessels.

Abstract：

:Laminin components were solubilized from basement membranes of amnion, chorion and chorionic microvessels of human placenta without prior protease digestion. These structures, after isolation, were initially processed in a sonicator bath containing a solution of Triton X-100, EDTA and 2M NaCl and the laminins extracted sequentially with 0.5M NaCl, 8 M urea, and 8 M urea + 2% 2-mercaptoethanol + 2% SDS. A high molecular weight (appr. 1 x 10(6)) complex containing laminins was purified by gel filtration on a Sepharose CL-2B column. This complex migrated as a single band on gel electrophoresis before reduction but resolved, after reduction, into four major laminin components, laminin A (350,000 M.W.), laminin M (240,000 M.W.) and laminins B1 and B2 (195,000 and 185,000 M.W.). Laminin M is a new molecular species of this protein.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Ohno M,Martinez-Hernandez A,Ohno N,Kefalides NA

doi

10.1016/0006-291x(83)91730-8

subject

Has Abstract

pub_date

1983-05-16 00:00:00

pages

1091-8

issue

3

eissn

0006-291X

issn

1090-2104

pii

0006-291X(83)91730-8

journal_volume

112

pub_type

杂志文章

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