Immunocytochemical localization of the Ca2+-ATPase polypeptide in human platelets.

abstract：:Functional role of CD44, a principal receptor of hyaluronan, and glycated albumin for differentiation of resting human monocytes isolated by counterflow centrifugal elutriation was investigated. Flow cytometric analysis revealed that amadori-modified glycated albumin induced expression of CD44 as well as macrophage sc...

journal_title：Biochemical and biophysical research communications

authors： Kishikawa H,Mine S,Kawahara C,Tabata T,Hirose A,Okada Y,Tanaka Y

更新日期：2006-01-20 00:00:00

abstract：:The hydrolysis of p-nitrophenylphosphorylcholine, p-nitrophenylphosphate, and phosphorylcholine was quantitated in homogenates prepared from the occipital and temporal regions of control and Alzheimer's Disease patient brain. There were no significant differences detected in activities of enzymes cleaving the first 2 ...

journal_title：Biochemical and biophysical research communications

authors： Kanfer JN,McCartney DG

更新日期：1986-08-29 00:00:00

abstract：:Although the neuron-restrictive silencer element (NRSE/Regard) has been shown to function as a negative-acting DNA regulatory element to prevent the expression of neuron-specific genes in non-neuronal cells, little is known about its silencing effect on transcription in primary glial cells nor its effect on transcript...

journal_title：Biochemical and biophysical research communications

authors： Tabuchi A,Nakatani C,Nakaoka R,Naruse Y,Kojima T,Mori N,Tsuda M

更新日期：1999-08-02 00:00:00

abstract：:4 beta phorbol-12, 13-dibutyrate (PDBu) stimulated cyclic AMP accumulation in GH3 pituitary tumour cells in the presence of isobutylmethylxanthine. This effect persisted after preincubation of cells with cholera or pertussis toxins. In contrast, vasoactive intestinal polypeptide (VIP)-stimulated cyclic AMP accumulatio...

journal_title：Biochemical and biophysical research communications

authors： Quilliam LA,Dobson PR,Brown BL

更新日期：1985-06-28 00:00:00

abstract：:Free energy changes for ATP synthesis (delta GP) and 2e(-)-transfer across Site 3 (delta GR) were determined during oxidative phosphorylation by rat liver mitochondria. At static head, -delta GR/delta GP ranged narrowly between 1.55 and 1.59 with five different respiratory substrates. Thus, an ATP/2e- of 1 1/2 at Site...

journal_title：Biochemical and biophysical research communications

authors： Lemasters JJ,Fleishman KE

更新日期：1987-01-15 00:00:00

abstract：:Oligo/poly alpha2,8 deaminoneuraminic acid (KDN), a unique posttranslational protein modification, was found on megalin and a not yet characterized 150 kDa glycoprotein. We purified this glycoprotein from rat testis and identified it as ceruloplasmin. Furthermore, immunoprecipitated ceruloplasmin from rat thymus, ovar...

journal_title：Biochemical and biophysical research communications

authors： Ziak M,Meier M,Novak-Hofer I,Roth J

更新日期：2002-07-19 00:00:00

abstract：:It has been proposed that the inositol 1,4,5-trisphosphate receptor (InsP(3)R) type III acts as a trigger for InsP(3)-mediated calcium (Ca(2+)) signaling, because this InsP(3) isoform lacks feedback inhibition by cytosolic Ca(2+). We tested this hypothesis in RIN-m5F cells, which express predominantly the type III rec...

journal_title：Biochemical and biophysical research communications

authors： O'Neill AF,Hagar RE,Zipfel WR,Nathanson MH,Ehrlich BE

更新日期：2002-06-14 00:00:00

abstract：:Neuroblastoma LAN-5 cells exposed to retinoic acid cease to multiply and extend neurite outgrowths acquiring a neuronal phenotype. We now report that protein kinase C-theta; (PKC-theta;) isozyme is involved in this differentiation process due to the following findings: (i) PKC-theta; is expressed by LAN-5 cells as a n...

journal_title：Biochemical and biophysical research communications

authors： Sparatore B,Patrone M,Passalacqua M,Pedrazzi M,Pontremoli S,Melloni E

更新日期：2000-12-20 00:00:00

abstract：:A fusion protein (bFGF-rMLA), containing the mitogen basic fibroblast growth factor (bFGF) and the cytotoxic component of rViscumin (recombinant mistletoe lectin), the enzymatic A-chain (rMLA), was expressed in Escherichia coli, purified, and functionally characterized. bFGF-rMLA is cytotoxic for mouse B16 melanoma ce...

journal_title：Biochemical and biophysical research communications

authors： Schmidt A,Möckel B,Eck J,Langer M,Gauert M,Zinke H

更新日期：2000-10-22 00:00:00

abstract：:Negative regulator of differentiation 1 (Nrd1) is known as a negative regulator of sexual differentiation in fission yeast. Recently, it has been revealed that Nrd1 also regulates cytokinesis, in which physical separation of the cell is achieved by a contractile ring comprising many proteins including actin and myosin...

journal_title：Biochemical and biophysical research communications

authors： Kobayashi A,Kanaba T,Satoh R,Fujiwara T,Ito Y,Sugiura R,Mishima M

更新日期：2013-07-19 00:00:00

abstract：:GPR35, previously an orphan G-protein coupled receptor, is a receptor for kynurenic acid. Here we examine the distribution of GPR35 in the rat dorsal root ganglion (DRG) and the effects of its selective activation. GPR35 was expressed predominantly by small- to medium-diameter neurons of the DRG. Many of these same ne...

journal_title：Biochemical and biophysical research communications

authors： Ohshiro H,Tonai-Kachi H,Ichikawa K

更新日期：2008-01-11 00:00:00

abstract：:Chemical modifications on human hemoglobin were performed with the aim to change both surface and active-site hydrophobicities. The modifications included covalent coupling of poly(ethylene)glycol (5000 MW) on free amino groups and the methyl esterification of free carboxylic groups. The modified hemoglobin was assaye...

journal_title：Biochemical and biophysical research communications

authors： Torres E,Vazquez-Duhalt R

更新日期：2000-07-14 00:00:00

abstract：:Many studies have been reported that tripartite motif-containing (TRIM) proteins play important roles in various cellular processes and involved in many diseases. TRIM3, a member of TRIM family, has been proved that it plays important roles in various cancers. Nevertheless, its effects on cervical cancer reminded unkn...

journal_title：Biochemical and biophysical research communications

authors： Song Y,Guo Q,Gao S,Hua K

更新日期：2018-04-06 00:00:00

abstract：:A protein, with a novel N-terminal amino acid sequence and a molecular mass of 30 kDa, was purified from fresh Smilax glabra rhizomes by adsorption on DEAE-cellulose, CM-cellulose, Con A-Sepharose, and Mono S, and by fast protein liquid chromatography-gel filtration on Superdex 75. The protein, designated as smilaxin,...

journal_title：Biochemical and biophysical research communications

authors： Chu KT,Ng TB

更新日期：2006-02-03 00:00:00

abstract：:The anti-glutathione antibody scFv 20C9, which we previously isolated from a human synthetic phage antibody scFv library [Hirose, M., Hayano, T., Shirai, H., Nakamura, H., and Kikuchi, M. (1998) Protein Eng. 11, 243-248], was expressed in the E. coli pET system and purified by sequential chromatography on Ni and gluta...

journal_title：Biochemical and biophysical research communications

authors： Horibe T,Furuya R,Iwai A,Yosho C,Tujimoto Y,Kikuchi M

更新日期：2001-03-01 00:00:00

abstract：:Two forms (G-I and G-II kinases) of casein kinase II(CK-II) in a partially purified CK-II fraction (Mono Q fraction) of mouse liver were separated by means of glycyrrhizin (GL)-affinity column chromatography. Biochemical characterization revealed that these two GL-binding kinases were identical to CK-II. Two phosphate...

journal_title：Biochemical and biophysical research communications

authors： Harada S,Karino A,Shimoyama Y,Shamsa F,Ohtsuki K

更新日期：1996-10-03 00:00:00

abstract：:Conjugated linoleic acid (CLA), a group of minor fatty acids from ruminant origin, has long been recognized as a body fat lowering agent. Given the trans(t)10,cis(c)12-CLA well documented interference on lipolysis, we hypothesized for adipocytes altered permeation to glycerol when supplemented with this isomer. 3T3-L1...

journal_title：Biochemical and biophysical research communications

authors： Martins SV,Madeira A,Lopes PA,Pires VM,Alfaia CM,Prates JA,Moura T,Soveral G

更新日期：2015-03-06 00:00:00

abstract：:Cholesterol is a critical component of lipid rafts implicated in regulating multiple signal transduction. The anthrax toxin receptor 2 (ANTXR2) is a type I membrane protein acting as the second receptor for the anthrax toxin. In this study, we first investigated the association between cholesterol and ANTXR2. We provi...

journal_title：Biochemical and biophysical research communications

authors： Zou J,Xu L,Ju Y,Zhang P,Wang Y,Zhang B

更新日期：2014-09-12 00:00:00

abstract：:The relative inability of hemopoietic stem cells (HSCs) to reproduce themselves (self-renew) ex vivo imposes substantial limitations on the current use of HSC transplantation. Recently, the transcription factor Zfx has been demonstrated that played an important in controlling the self-renewal of hematopoietic stem cel...

journal_title：Biochemical and biophysical research communications

authors： Xu C,Zhang Y,Jiang H

更新日期：2009-02-13 00:00:00

abstract：:Glycogen phosphorylase in PC12 cells exists in two forms analogous to those found in brain and muscle. The active phosphorylated form of the enzyme, phosphorylase-a, represents about 20-30% of total glycogen phosphorylase in these cells. Incubation of PC12 cells with 100 ng 7S nerve growth factor/ml increased phosphor...

journal_title：Biochemical and biophysical research communications

authors： Davis LH,Kauffman FC

更新日期：1986-07-31 00:00:00

abstract：:Prolactin, the Janus kinase 2 (JAK2) and the signal transducer and activator of transcription 5 (STAT5) are important for mammary gland development and have also been implicated in development and growth of breast tumors. In the present study we have investigated the role for JAK2 in proliferation, differentiation, an...

journal_title：Biochemical and biophysical research communications

authors： Olsen H,Hedengran Faulds MA,Saharinen P,Silvennoinen O,Haldosén LA

更新日期：2002-08-09 00:00:00

abstract：:NMR spectroscopy is used to compare the structure of the EGF-like domain of heregulin-alpha and HT1, a mutated form of heregulin-alpha with significantly reduced activity. HT1 is a chimeric protein that has the first seven residues of transforming growth factor-alpha and the sequence of heregulin-alpha from the first ...

journal_title：Biochemical and biophysical research communications

authors： Adler M,Thompson SA

更新日期：1999-03-05 00:00:00

abstract：:Histamine induced a concentration-dependent increase in intracellular cyclic-AMP of the two human melanoma cell lines SK23 and DX3.LT5.1; maximal stimulation was obtained with 17.8 microM histamine which consistently produced greater than 50-fold increases in the cyclic AMP content of both cell lines. The dose-respons...

journal_title：Biochemical and biophysical research communications

authors： Whitehead RJ,Taylor DJ,Evanson JM,Hart IR,Woolley DE

更新日期：1988-02-29 00:00:00

abstract：:We have isolated and characterized the cDNA encoding a novel protein designated DJ-1. DJ-1, sharing no significant homology with the sequences so far reported, did not show transactivation activity in the Gal4 recombinant system, but transformed mouse NIH3T3 cells by itself. Furthermore, DJ-1 showed a cooperative tran...

journal_title：Biochemical and biophysical research communications

authors： Nagakubo D,Taira T,Kitaura H,Ikeda M,Tamai K,Iguchi-Ariga SM,Ariga H

更新日期：1997-02-13 00:00:00

abstract：:Sequence analysis of a new lignin peroxidase (LIP) gene, GLG6, from P. chrysosporium showed that it encodes a mature LIP protein with a predicted Mr of 36,454. A 28 aa signal peptide precedes the mature protein. The coding region of GLG6 is interrupted by nine introns ranging in size from 50-57 bp. GLG6 encoded-LIP ha...

journal_title：Biochemical and biophysical research communications

authors： Naidu PS,Zhang YZ,Reddy CA

更新日期：1990-12-31 00:00:00

abstract：:Activation of protein kinase C has been shown to be involved in the activation pathway of many cell types. Recently, a number of investigations have suggested that protein kinase C plays an essential role in T lymphocyte activation. The recent synthesis of the protein kinase inhibitors, H-7 and HA1004, have now made p...

journal_title：Biochemical and biophysical research communications

authors： Clark RB,Love JT Jr,Sgroi D,Lingenheld EG,Sha'afi RI

更新日期：1987-06-15 00:00:00

abstract：:SIRT5 is one of the seven mammalian sirtuins which are NAD+-dependent deacylases. In human beings, SIRT5 gene encodes for four SIRT5 protein isoforms, namely SIRT5iso1, SIRT5iso2, SIRT5iso3, and SIRT5iso4. Previous studies have focused mostly on SIRT5iso1. Characteristics regarding localization, activity and tissue di...

journal_title：Biochemical and biophysical research communications

authors： Du Y,Hu H,Hua C,Du K,Wei T

更新日期：2018-09-05 00:00:00

abstract：:Brewers yeast contains factors that increase and decrease glucose tolerance. Hop components (lupulones) that adhere to yeast during the brewing process elicit a variety of biological effects including the induction of hepatic cytochrome P4503A. Colupulone was tested for its effects on glucose tolerance and cytochrome ...

journal_title：Biochemical and biophysical research communications

authors： Mannering GJ,Shoeman JA,Shoeman DW

更新日期：1994-05-16 00:00:00

abstract：:RNA polymerase III promoters of human ribonuclease P RNA component H1, human U6, and mouse U6 small nuclear RNA genes are commonly used in short hairpin RNA (shRNA) expression vectors due their precise initiation and termination sites. During transient transfection of shRNA vectors, we observed that H1 or U6 promoters...

journal_title：Biochemical and biophysical research communications

authors： Rumi M,Ishihara S,Aziz M,Kazumori H,Ishimura N,Yuki T,Kadota C,Kadowaki Y,Kinoshita Y

更新日期：2006-01-13 00:00:00

abstract：:Inspired by recent interest in torsion of the central pair microtubules in eukaryotic flagella, a novel thin-walled elastic beam model is suggested to study critical condition under which uniform bending of a flagellum will cause lateral/torsional buckling of the central pair. The model is directed to the central pair...

journal_title：Biochemical and biophysical research communications

authors： Li C,Ru CQ,Mioduchowski A

更新日期：2006-12-08 00:00:00