Abstract:
:Neuroblastoma LAN-5 cells exposed to retinoic acid cease to multiply and extend neurite outgrowths acquiring a neuronal phenotype. We now report that protein kinase C-theta; (PKC-theta;) isozyme is involved in this differentiation process due to the following findings: (i) PKC-theta; is expressed by LAN-5 cells as a nuclear and perinuclear protein; (ii) cell stimulation with retinoic acid promotes in a large increase in the expression level of the kinase and its intracellular redistribution; and (iii) a PKC-theta; antisense oligonucleotide reduces at the same time the expression level of the kinase and the cell response to retinoic acid. Altogether these data are consistent with a specific role played by PKC-theta; in the differentiation program of neuronal cells.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Sparatore B,Patrone M,Passalacqua M,Pedrazzi M,Pontremoli S,Melloni Edoi
10.1006/bbrc.2000.3950subject
Has Abstractpub_date
2000-12-20 00:00:00pages
589-94issue
2eissn
0006-291Xissn
1090-2104pii
S0006-291X(00)93950-0journal_volume
279pub_type
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