The mosquito larvicidal activity of 130 kDa delta-endotoxin of Bacillus thuringiensis var. israelensis resides in the 72 kDa amino-terminal fragment.

abstract：:The conditions used in some immunoblot procedures can fail to detect calmodulin, S100 proteins, and other proteins with similar physical properties. We describe here some of the basis of this difficulty, and provide an immunoblot protocol that allows the rapid and reproducible detection of calmodulin and S100 beta in ...

journal_title：Biochemical and biophysical research communications

authors： Van Eldik LJ,Wolchok SR

更新日期：1984-11-14 00:00:00

abstract：:Human serum albumin (HSA) serves as a natural depot of amyloid β peptide (Aβ). Improvement of Aβ binding to HSA should impede Alzheimer's disease (AD). We developed a method for quantitation of the interaction between monomeric Aβ40/42 and HSA using surface plasmon resonance spectroscopy. The dissociation constant of ...

journal_title：Biochemical and biophysical research communications

authors： Litus EA,Kazakov AS,Sokolov AS,Nemashkalova EL,Galushko EI,Dzhus UF,Marchenkov VV,Galzitskaya OV,Permyakov EA,Permyakov SE

更新日期：2019-03-05 00:00:00

abstract：:Mycobacterium smegmatis is a good model for studying the physiology and pathogenesis of Mycobacterium tuberculosis due to its genetic similarity. As methionine biosynthesis exists only in microorganisms, the enzymes involved in methionine biosynthesis can be a potential target for novel antibiotics. Homoserine O-acety...

journal_title：Biochemical and biophysical research communications

authors： Sagong HY,Hong J,Kim KJ

更新日期：2019-09-24 00:00:00

abstract：:The aromatic l-alpha-hydroxy acid dehydrogenase (AHDAH) from Trypanosoma cruzi has over 50% sequence identity with cytosolic malate dehydrogenases (cMDHs), yet it is unable to reduce oxaloacetate. Molecular modeling of the three-dimensional structure of AHADH using the pig cMDH as template directed the construction of...

journal_title：Biochemical and biophysical research communications

authors： Vernal J,Fiser A,Sali A,Müller M,Cazzulo JJ,Nowicki C

更新日期：2002-04-26 00:00:00

abstract：:Voltage-dependent anion channel (VDAC) is a multi-functional channel protein in the mitochondrial outer membrane of all eukaryotes. It is involved in extensive physiological and pathophysiological processes. However there is only scant information about VDAC in mammalian reproduction, fertility and development in the ...

journal_title：Biochemical and biophysical research communications

authors： Liu B,Zhang W,Wang Z

更新日期：2010-07-09 00:00:00

abstract：:Beta A3/A1-crystallin is one of the major refractive proteins of the chicken eye lens. Previously we showed that a fragment from -382 to +22 bp of the beta A3/A1-crystallin gene functions as a promoter in transfected lens cells. Here we show by use of the bacterial chloramphenicol acetyltransferase reporter gene that ...

journal_title：Biochemical and biophysical research communications

authors： McDermott JB,Cvekl A,Piatigorsky J

更新日期：1996-04-25 00:00:00

abstract：:A system for the expression and purification of soluble VP8*, part of the human rotavirus (HRV) spike protein, was established by expressing VP8* as a fusion protein with glutathione S-transferase (GST). VP8 cDNA, from the Wa strain of HRV, was prepared by RT-PCR, cloned into a pUC18 plasmid, and inserted into a pGEX-...

journal_title：Biochemical and biophysical research communications

authors： Kovacs-Nolan J,Sasaki E,Yoo D,Mine Y

更新日期：2001-04-20 00:00:00

abstract：:We have previously reported the identification of two closely related RNA binding proteins from Trypanosoma brucei which we have termed p34 and p37. The predicted primary structures of the two proteins are highly homologous with one major difference, an 18-amino-acid insert in the N-terminal region of p37. These two p...

journal_title：Biochemical and biophysical research communications

authors： Pitula J,Ruyechan WT,Williams N

更新日期：2002-01-11 00:00:00

abstract：:The cellular molecular motor kinesin-1 mediates the microtubule-dependent transport of a range of cargo. We have previously identified an interaction between the cargo-binding domain of kinesin-1 heavy chain KIF5B and the membrane-associated SNARE proteins SNAP-25 and SNAP-23. In this study we further defined the mini...

journal_title：Biochemical and biophysical research communications

authors： Morton AM,Cunningham AL,Diefenbach RJ

更新日期：2010-01-01 00:00:00

abstract：:Light-induced conformational changes occurring at the cytosolic surface of rhodopsin were investigated by performing limited digestions of native and illuminated visual pigment with thermolysin, Arg-C endoproteinase, papain and proteinase K. A higher susceptibility of the extradiscal regions of the bleached pigment to...

journal_title：Biochemical and biophysical research communications

authors： Pellicone C,Nullans G,Cook NJ,Virmaux N

更新日期：1985-03-29 00:00:00

abstract：:Plastocyanin (PC) is a copper protein that serves as a mobile electron carrier between cytochrome f and Photosystem I in the light reactions of photosynthesis. Despite large variability in amino acid sequences and isoelectric points, PCs from cyanobacterial and chloroplast sources reveal considerable similarities with...

journal_title：Biochemical and biophysical research communications

authors： Babu CR,Arudchandran A,Hille R,Gross EL,Bullerjahn GS

更新日期：1997-06-27 00:00:00

abstract：:Translational control by specific eIF2alpha phosphorylation on serine 51 has been characterized in all eukaryotes with the significant exception of plants. In order to evaluate the capability of plant eIF2alpha to functionally control translation, the wild type (51S) and a nonphosphorylatable mutant (51A) of wheat eIF...

journal_title：Biochemical and biophysical research communications

authors： Chang LY,Yang WY,Roth D

更新日期：2000-12-20 00:00:00

abstract：:ATP-binding cassette sub-family G member 2 (ABCG2) confers to the major phenotypes of side population (SP) cells, the cancer stem-like cells. In this study, the SP cells displayed a distinctly higher ABCG2 expression level, sphere formation efficiency (SFE) and growth rate even under hypoxia condition. CXCR4 overexpre...

journal_title：Biochemical and biophysical research communications

authors： He C,Zhang H,Wang B,He J,Ge G

更新日期：2018-10-28 00:00:00

abstract：:Dynamic properties of spin-labelled heterosaccharides in the Fc-region of murine monoclonal antihapten immunoglobulin G were studied in model immune complexes (IC) as a function of the IC size. Model IC dimers, trimers and oligomers were formed using bivalent photoaffinity antigens. The ESR spectrum exhibits two compo...

journal_title：Biochemical and biophysical research communications

authors： Kusumi A,Winkelhake JL

更新日期：1986-05-29 00:00:00

abstract：:In a survey for unknown bioactive peptides in mammalian cell lines, we isolated peptides exhibiting a strong relaxant effect on chick rectum from a phorbol ester-supplemented culture medium of the human monocytic cell line, THP-1. The peptide was deduced to be a C-terminal 29-residue peptide derived from a human C-typ...

journal_title：Biochemical and biophysical research communications

authors： Ishizaka Y,Kangawa K,Minamino N,Ishii K,Takano S,Eto T,Matsuo H

更新日期：1992-12-15 00:00:00

abstract：:Mutations in the WNK1 and WNK4 genes result in an inherited hypertensive disease, pseudohypoaldosteronism type II (PHAII). Recently, the KLHL3 and Cullin3 genes were also identified as responsible genes for PHAII. Although we have reported that WNK4 is a substrate for the KLHL3-Cullin3 E3 ligase complex, it is not cle...

journal_title：Biochemical and biophysical research communications

authors： Takahashi D,Mori T,Wakabayashi M,Mori Y,Susa K,Zeniya M,Sohara E,Rai T,Sasaki S,Uchida S

更新日期：2013-08-02 00:00:00

abstract：:The mouse disabled 2 interacting protein 2 (mDaIP2) had been obtained through yeast two hybrid system. It consists of 506 amino acids and its calculated molecular weight is 57.7 kDa. The protein contains N-terminal FCH domain and C-terminal SH3 domain. The SH3 domain interacts with the proline rich domain of mDab2 whi...

journal_title：Biochemical and biophysical research communications

authors： Choi YJ,Cho SY,Kim HW,Kim JA,Bae SH,Park SS

更新日期：2005-01-21 00:00:00

abstract：:Pyruvate dehydrogenase kinase (PDK) specifically phosphorylates the E1alpha subunit of the pyruvate dehydrogenase complex (PDC). Sequence analysis of cloned PDKs led to the proposal that they are mechanistically related to prokaryotic 2-component His-kinases. The reaction mechanism of protein His-kinases involves auto...

journal_title：Biochemical and biophysical research communications

authors： Mooney BP,David NR,Thelen JJ,Miernyk JA,Randall DD

更新日期：2000-01-19 00:00:00

abstract：:Helodermin is a peptide from the venom of the lizard Heloderma suspectum (Gila Monster) showing a high degree of sequence similarity with VIP, PHI and secretin in its N-terminal moiety. The present data support the presence of peptide(s) closely related to helodermin in the brain, gut and salivary glands of rat. In ou...

journal_title：Biochemical and biophysical research communications

authors： Robberecht P,De Graef J,Woussen MC,Vandermeers-Piret MC,Vandermeers A,De Neef P,Cauvin A,Yanaihara C,Yanaihara N,Christophe J

更新日期：1985-07-16 00:00:00

abstract：:Porins are trimeric proteins in the outer membranes of Gram-negative bacteria. Several of them, among them matrix porin of Escherichia coli, form symmetrically voltage-gated ion channels in planar bilayers. Trimers exhibit negative resistance at potentials larger than +/- 90mV. Here we show that, after two pores withi...

journal_title：Biochemical and biophysical research communications

authors： Buehler LK,Rosenbusch JP

更新日期：1993-01-29 00:00:00

abstract：:The carbocyclic analogue of (E)-5-(2-bromovinyl)-2'-deoxyuridine, C-BVDU, is a very potent and selective anti-herpes-virus compound. In order to synthesize and study the properties of a DNA that contains C-BVDU, the 5'-triphosphate, C-BVDUTP was prepared and evaluated as a potential substrate of the E. coli Klenow DNA...

journal_title：Biochemical and biophysical research communications

authors： Sági J,De Clercq E,Szemzö A,Csárnyi A,Kovács T,Otvös L

更新日期：1987-09-30 00:00:00

abstract：:Carp acclimated to 10 degrees C gave 69k, 66k, and 62kDa light meromyosin (LMM) fragments in SDS-PAGE, while fish acclimated to 30 degrees C gave 74k, 69k, 66k, and 62kDa fragments. The microsequence analysis revealed that the 69k and 66kDa components from the 10 degrees C-acclimated carp contained an N-terminal amino...

journal_title：Biochemical and biophysical research communications

authors： Watabe S,Imai J,Nakaya M,Hirayama Y,Okamoto Y,Masaki H,Uozumi T,Hirono I,Aoki T

更新日期：1995-03-08 00:00:00

abstract：:Cytochrome P450 enzymes (CYP enzymes) catalyse the initial step in biotransformation of xenobiotics like polycyclic aromatic hydrocarbons (PAHs). The marine polychaete Nereis virens has a high capacity for biotransformation of PAHs. In the present study, the complete cDNA sequences of two novel CYP genes isolated from...

journal_title：Biochemical and biophysical research communications

authors： Jørgensen A,Rasmussen LJ,Andersen O

更新日期：2005-10-28 00:00:00

abstract：:p73, a member of the p53 family, is a transcription factor that plays a key role in many biological processes. In the present study, we show that TAp73 is expressed in neural stem cells (NSC) and its expression increases following their differentiation. NSC from p73 null mice have a reduced proliferative potential, to...

journal_title：Biochemical and biophysical research communications

authors： Agostini M,Tucci P,Chen H,Knight RA,Bano D,Nicotera P,McKeon F,Melino G

更新日期：2010-12-03 00:00:00

abstract：:The process of endochondral ossification is strictly regulated by a variety of extracellular and intracellular factors. Recently, it has become recognized that specific miRNAs are involved in this process by regulating the expression of the relevant genes at the post-transcriptional level. In this present study we obt...

journal_title：Biochemical and biophysical research communications

authors： Sumiyoshi K,Kubota S,Ohgawara T,Kawata K,Nishida T,Shimo T,Yamashiro T,Takigawa M

更新日期：2010-11-12 00:00:00

abstract：:We have isolated calpromotin, a protein reported abundant in human red cells and shown to be of significance for KCl transport. We show that calpromotin is identical to a radical scavenger protein, thiol-specific antioxidant protein (TSA). Calpromotin is known to exist partially as a large complex of identical subunit...

journal_title：Biochemical and biophysical research communications

authors： Kristensen P,Rasmussen DE,Kristensen BI

更新日期：1999-08-19 00:00:00

abstract：:Chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase was expressed in E. coli by using a pET3a T7 RNA polymerase-based expression system and was purified to homogeneity. The kinase and bisphosphatase of the expressed bifunctional enzyme had kinetic properties identical to those of the native chicken live...

journal_title：Biochemical and biophysical research communications

authors： Li L,Yao WZ,Lange AJ,Pilkis SJ,Dong MQ,Yin Y,Xu GJ

更新日期：1995-04-26 00:00:00

abstract：:The extended exposure of proteins to reducing sugars leads to nonenzymatic glycation with the accumulation of advanced glycation end products (AGEs). Long-lived proteins, such as collagen and crystallins, are subjected to this modification, and are implicated as causal factors in several diseases including diabetic co...

journal_title：Biochemical and biophysical research communications

authors： Hong SB,Lee KW,Handa JT,Joo CK

更新日期：2000-08-18 00:00:00

abstract：:Tumor necrosis factor-alpha (TNFα) is a multifunctional cytokine associated with inflammation, immune responses, and autoimmune diseases including rheumatoid arthritis, inflammatory bowel disease, and psoriasis. In the present study, we performed in vitro selection, systematic evolution of ligands by exponential enric...

journal_title：Biochemical and biophysical research communications

authors： Tsukamoto K,Ando T,Fuji D,Yokoyama T,Takamori Y,Horiuchi D,Iwamoto R,Yamamoto M,Kawakami T

更新日期：2021-01-01 00:00:00

abstract：:The immunoglobulin G of a polyclonal antiserum (pIgG) from a patient with insulin resistance and hypoglycemia was tested for its ability to inhibit insulin binding and to affect the autophosphorylation of partially-purified insulin receptors extracted from rat liver membranes. pIgG, when added 4 hr prior to insulin, i...

journal_title：Biochemical and biophysical research communications

authors： Cordera R,Gherzi R,De Pirro R,Rossetti L,Freidenberg GR,Andraghetti G,Lauro R,Adezati L

更新日期：1985-11-15 00:00:00