Abstract:
:In a survey for unknown bioactive peptides in mammalian cell lines, we isolated peptides exhibiting a strong relaxant effect on chick rectum from a phorbol ester-supplemented culture medium of the human monocytic cell line, THP-1. The peptide was deduced to be a C-terminal 29-residue peptide derived from a human C-type natriuretic peptide (CNP) precursor. CNP mRNA was also detected in the THP-1 cells, and expression of CNP gene and CNP concentration in the culture medium was found to be highly augmented by the stimulation of phorbol ester. Production of CNP in THP-1 cells suggests that CNP also functions as a local regulator in the blood cell-vascular system, although CNP has previously been recognized as a neuropeptide functioning in the central nervous system.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Ishizaka Y,Kangawa K,Minamino N,Ishii K,Takano S,Eto T,Matsuo Hdoi
10.1016/0006-291x(92)92257-xsubject
Has Abstractpub_date
1992-12-15 00:00:00pages
697-704issue
2eissn
0006-291Xissn
1090-2104pii
0006-291X(92)92257-Xjournal_volume
189pub_type
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