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Specific packaging of spliced retroviral vector transcripts lacking the Psi-region.

Abstract：

:The effect of a cryptic splice acceptor (cSA) site located at the end of the extended packaging signal in murine leukemia virus (MLV)-based vectors was investigated. Although this cSA is also present in wild type MLV, it was found to result in a smaller transcript in which the packaging signal (Psi) had been removed by splicing only in MLV-derived vectors. Splicing occurs both in packaging cells producing the MLV-vectors as well as in the infected target cells. Transcripts lacking the Psi-sequence (Psi(-)) are packaged relatively efficiently into virus particles, even in the presence of wild type Psi(+)-vector transcripts. The Psi(-)-viral RNA is reverse transcribed in vector transduced cells as is any other retroviral genome. The titer obtained from the Psi(-)-vector was only 1000-fold reduced in comparison to the same Psi(+)-vector. These results suggest that Psi(-)-transcripts may be packaged more frequently than previously supposed and that splicing patterns should be carefully analysed on an individual basis for retroviral vectors used in gene therapy.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Pambalk K,Hohenadl C,Salmons B,Günzburg WH,Renner M

doi

10.1016/S0006-291X(02)00207-3

subject

Has Abstract

pub_date

2002-04-26 00:00:00

pages

239-46

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(02)00207-3

journal_volume

293

pub_type

杂志文章

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