Abstract:
:Two repeated C2-like domains interacting with Ca2+ and phospholipid are found in synaptotagmin and Rabphilin-3A which are implicated in neurotransmitter release. Here we have isolated a cDNA encoding a novel protein having two repeated C2-like domains from a human brain cDNA library. The isolated cDNA encodes a protein with 400 amino acids and a M(r) of 44,071. The purified recombinant protein indeed interacts with Ca2+ and phospholipid. We have named this protein Doc2 (Double C2). Doc2 is exclusively expressed in brain and is highly concentrated in the synaptic vesicle fraction. These results suggest that Doc2 is a novel brain protein and serves as a Ca2+ sensor in neurotransmitter release.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Orita S,Sasaki T,Naito A,Komuro R,Ohtsuka T,Maeda M,Suzuki H,Igarashi H,Takai Ydoi
10.1006/bbrc.1995.1062subject
Has Abstractpub_date
1995-01-17 00:00:00pages
439-48issue
2eissn
0006-291Xissn
1090-2104pii
S0006-291X(85)71062-5journal_volume
206pub_type
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