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Development of a single-cassette system for spatiotemporal gene regulation in mice.

Abstract：

:The tetracycline-regulated gene expression system has been widely used in mice to turn a transgene on and off in a target organ, but with only limited success. We developed an advanced system in which a Tet-off regulation unit was integrated into the ROSA26 locus and became active after Cre-mediated excision of the neo(r) gene. We examined the utility of this system through regulable expression of the homeodomain transcription factor pdx-1 and enhanced green fluorescent protein. The resulting mice showed strict tetracycline-regulable gene expression in all the organs where the neo(r) gene had been removed. When combined with organ-specific Cre recombinase transgenic mice, our system allows us to manipulate the gene expression in an organ-specific and temporal manner. This Tet-off system should serve as an efficient tool to analyze the roles of genes in complex biological systems, such as embryogenesis, metabolism, immune system, etc.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Miyazaki S,Miyazaki T,Tashiro F,Yamato E,Miyazaki J

doi

10.1016/j.bbrc.2005.10.054

subject

Has Abstract

pub_date

2005-12-16 00:00:00

pages

1083-8

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(05)02303-X

journal_volume

338

pub_type

杂志文章

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