当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > The receptor and transporter for internalization of Clostridium botulinum type C progenitor toxin into HT-29 cells.

The receptor and transporter for internalization of Clostridium botulinum type C progenitor toxin into HT-29 cells.

Abstract：

:Orally ingested botulinum toxin enters the circulatory system and eventually reaches the peripheral nerves, where it elicits a response of neurological dysfunction. In this study, we report the important findings concerning the mechanism of Clostridium botulinum type C progenitor toxin (C16S) endocytic mechanism. C16S toxin bound to high molecular weight proteins on the surface of human colon carcinoma HT-29 cells and was internalized, but not if the cells were pretreated with neuraminidase. Benzyl-GalNAc which inhibited O-glycosylation of glycoproteins also interfered in the toxin's ability to bind the cell surface. On the other hand, the toxin was internalized in spite of pretreatment of the cells with PPMP, an inhibitor of ganglioside synthesis. These results suggest that the glycoproteins, like mucin, fulfill the important roles of receptor and transporter of C16S toxin.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Nishikawa A,Uotsu N,Arimitsu H,Lee JC,Miura Y,Fujinaga Y,Nakada H,Watanabe T,Ohyama T,Sakano Y,Oguma K

doi

10.1016/j.bbrc.2004.04.183

subject

Has Abstract

pub_date

2004-06-25 00:00:00

pages

327-33

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006291X04009507

journal_volume

319

pub_type

杂志文章

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