Glioma inhibition by HGF/NK2, an antagonist of scatter factor/hepatocyte growth factor.

Abstract:

:Strategies that antagonize growth factor signaling are attractive candidates for the biological therapy of brain tumors. HGF/NK2 is a secreted truncated splicing variant and potential antagonist of scatter factor/hepatocyte growth factor (SF/HGF), a multifunctional cytokine involved in the malignant progression of solid tumors including glioblastoma. U87 human malignant glioma cells that express an autocrine SF/HGF stimulatory loop were transfected with the human HGF/NK2 cDNA and clonal cell lines that secrete high levels of HGF/NK2 protein (U87-NK2) were isolated. The effects of HGF/NK2 gene transfer on the U87 malignant phenotype were examined. HGF/NK2 gene transfer had no effect on 2-dimensional anchorage-dependent cell growth. In contrast, U87-NK2 cell lines were approximately 20-fold less clonogenic in soft agar and approximately 4-fold less migratory than control-transfected cell lines. Intracranial tumor xenografts derived from U87-NK2 cells grew much slower than controls. U87-NK2 tumors were approximately 50-fold smaller than controls at 21 days post-implantation and HGF/NK2 gene transfer resulted in a trend toward diminished tumorigenicity. This report shows that the predominant effect of transgenic HGF/NK2 overexpression by glioma cells that are autocrine for SF/HGF stimulation is to inhibit their malignant phenotype.

authors

Guerin C,Luddy C,Abounader R,Lal B,Laterra J

doi

10.1006/bbrc.2000.2935

subject

Has Abstract

pub_date

2000-06-24 00:00:00

pages

287-93

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(00)92935-8

journal_volume

273

pub_type

杂志文章
  • Mature adipocyte-derived dedifferentiated fat cells can transdifferentiate into skeletal myocytes in vitro.

    abstract::We have previously reported the establishment of preadipocyte cell lines, termed dedifferentiated fat (DFAT) cells, from mature adipocytes of various animals. DFAT cells possess long-term viability and can redifferentiate into adipocytes both in vivo and in vitro. Furthermore, DFAT cells can transdifferentiate into os...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.10.046

    authors: Kazama T,Fujie M,Endo T,Kano K

    更新日期:2008-12-19 00:00:00

  • Epigenetic regulation of CFTR in salivary gland.

    abstract::Cystic fibrosis transmembrane conductance regulator (CFTR) plays a key role in exocrine secretion, including salivary glands. However, its functional expression in salivary glands has not been rigorously studied. In this study, we investigated the expression pattern and regulatory mechanism of CFTR in salivary glands ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2016.11.023

    authors: Shin YH,Lee SW,Kim M,Choi SY,Cong X,Yu GY,Park K

    更新日期:2016-12-02 00:00:00

  • The nuclear receptor and clock gene REV-ERBα regulates cigarette smoke-induced lung inflammation.

    abstract::REV-ERBα is a nuclear heme receptor, transcriptional repressor and critical component of the molecular clock that drives daily rhythms of metabolism. Evidence reveals that REV-ERBα also plays an important regulatory role in clock-dependent lung physiology and inflammatory responses. We hypothesize that cigarette smoke...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2017.09.157

    authors: Sundar IK,Rashid K,Sellix MT,Rahman I

    更新日期:2017-12-02 00:00:00

  • Multiple mechanisms of iron-induced ferritin synthesis in HeLa cells.

    abstract::Iron administration to HeLa cells stimulates the accumulation of H-subunit and L-subunit rich isoferritins at similar extent. The increase in both types of isoferritins is accompanied by an increase in the amount of messenger RNAs specific for H and L subunits. The increase in the amount of these messenger RNAs, which...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(85)91877-7

    authors: Cairo G,Bardella L,Schiaffonati L,Arosio P,Levi S,Bernelli-Zazzera A

    更新日期:1985-11-27 00:00:00

  • Reduction in tumor necrosis factor receptor affinity and cytotoxicity by glucocorticoids.

    abstract::Micromolar concentrations of glucocorticoids rendered L-M cells (a murine tumorigenic fibroblast line) less sensitive to the cytotoxic activity of murine TNF. The potency of different steroids paralleled their known anti-inflammatory potency, and pretreatment was more effective than post treatment. Sex steroids and mi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(88)81238-5

    authors: Kull FC Jr

    更新日期:1988-05-31 00:00:00

  • BNIPL-2, a novel homologue of BNIP-2, interacts with Bcl-2 and Cdc42GAP in apoptosis.

    abstract::The execution phase of apoptosis is characterized by marked changes in cell morphology that include contraction and membrane blebbing. Little is known about the mechanisms underlying this process. We report here the identification of a novel member of BNIPL family, designated Bcl-2/adenovirus E1B 19kDa interacting pro...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(03)01387-1

    authors: Qin W,Hu J,Guo M,Xu J,Li J,Yao G,Zhou X,Jiang H,Zhang P,Shen L,Wan D,Gu J

    更新日期:2003-08-22 00:00:00

  • Demonstration of a basic fibroblast growth factor-like molecule in mouse hepatic endothelial cells.

    abstract::We have investigated whether isolated mouse hepatic sinusoidal endothelial cells (HSEC) synthesized basic FGF. HSEC lysate was fractionated by heparin-Sepharose chromatography. A peak of mitogenic activity for Balb/c 3T3 fibroblasts was eluted with 3M NaCl. Several arguments suggested that the mitogenic factor was rel...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(89)91782-8

    authors: Rosenbaum J,Mavier P,Preaux AM,Dhumeaux D

    更新日期:1989-11-15 00:00:00

  • Silencing IFNγ inhibits A1 astrocytes and attenuates neurogenesis decline and cognitive impairment in endotoxemia.

    abstract::Cognitive impairment, acute or long-term, is a common complication in patients with severe bacterial infection. However, the underlying mechanisms are not fully verified and effective medicine is not available in clinics. Interferon gamma (IFNγ) is a pivotal cytokine against infection and is believed to be a tune in h...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2020.10.084

    authors: Lu Y,Yang Y,Peng Z,Xie L,Zhong X,Liang F,Yuan C,Lu B

    更新日期:2020-12-17 00:00:00

  • Synthesis and biological actions of melanin concentrating hormone.

    abstract::A melanin (melanosome) concentrating hormone, MCH, was synthesized and the methodology for its synthesis is detailed. This heptadecapeptide, H-Asp-Thr-Met-Arg-Cys-Met-Val-Gly-Arg-Val-Tyr-Arg-Pro-Cys-Trp-Glu-Val-OH , stimulated melanosome concentration (centripetal aggregation) within melanophores of all species of tel...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(84)80077-7

    authors: Wilkes BC,Hruby VJ,Sherbrooke WC,Castrucci AM,Hadley ME

    更新日期:1984-07-31 00:00:00

  • Valine, the branched-chain amino acid, suppresses hepatitis C virus RNA replication but promotes infectious particle formation.

    abstract:BACKGROUND & AIMS:Concentrations of the branched-chain amino acid (BCAA) in the serum of patients with liver cirrhosis correlate with their liver function. Oral administration of BCAA can ameliorate hypoalbuminemia and hepatic encephalopathy. In this study, we aim to clarify the role of BCAA in regulating the replicati...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2013.06.051

    authors: Ishida H,Kato T,Takehana K,Tatsumi T,Hosui A,Nawa T,Kodama T,Shimizu S,Hikita H,Hiramatsu N,Kanto T,Hayashi N,Takehara T

    更新日期:2013-07-19 00:00:00

  • Prevention of intracellular degradation of I2020T mutant LRRK2 restores its protectivity against apoptosis.

    abstract::Leucine-rich repeat kinase 2 (LRRK2) is the causal gene for autosomal dominant familial Parkinson's disease. We have previously reported a novel molecular feature characteristic to I2020T mutant LRRK2: higher susceptibility to post-translational degradation than the wild-type LRRK2. In the present study, we demonstrat...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2009.11.043

    authors: Ohta E,Kubo M,Obata F

    更新日期:2010-01-01 00:00:00

  • Insulin/NFκB protects against ischemia-induced necrotic cardiomyocyte death.

    abstract::In the heart, insulin controls key functions such as metabolism, muscle contraction and cell death. However, all studies have been focused on insulin action during reperfusion. Here we explore the cardioprotective action of this hormone during ischemia. Rat hearts were perfused ex vivo with an ischemia/reperfusion Lan...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2015.09.171

    authors: Díaz A,Humeres C,González V,Gómez MT,Montt N,Sanchez G,Chiong M,García L

    更新日期:2015-11-13 00:00:00

  • WITHDRAWN: Hexavalent chromium induces premature senescence through reactive oxygen species-mediated p53 pathway in L-02 hepatocytes.

    abstract::This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2012.04.044

    authors: Xiao F,Hu Q,Zeng M,Guan L,Liu X,Yang Y,Zhong C

    更新日期:2012-04-13 00:00:00

  • Nek2 localizes to multiple sites in mitotic cells, suggesting its involvement in multiple cellular functions during the cell cycle.

    abstract::Nek2 is a mammalian protein kinase that is structurally homologous to NIMA, a mitotic regulator in Aspergillus nidulans. To understand the possible cellular processes in which Nek2 participates during the cell cycle, we investigated the expression and subcellular localization of Nek2 in mitotic cells. The Nek2 protein...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2001.6212

    authors: Ha Kim Y,Yeol Choi J,Jeong Y,Wolgemuth DJ,Rhee K

    更新日期:2002-01-18 00:00:00

  • Cloning and functional characterization of ACAD-9, a novel member of human acyl-CoA dehydrogenase family.

    abstract::Acyl-CoA dehydrogenases (ACADs) are a family of mitochondrial enzymes catalyzing the initial rate-limiting step in the beta-oxidation of fatty acyl-CoA. The reaction provides main source of energy for human heart and skeletal muscle. Eight human ACADs have been described. Deficiency of these enzymes, especially very l...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(02)02336-7

    authors: Zhang J,Zhang W,Zou D,Chen G,Wan T,Zhang M,Cao X

    更新日期:2002-10-04 00:00:00

  • Stimulation of calcium entry is prerequisite for DNA synthesis induced by platelet-derived growth factor in vascular smooth muscle cells.

    abstract::The present study was conducted to determine a role of calcium entry in the growth promoting action of platelet-derived growth factor (PDGF) in cultured rat vascular smooth muscle cells. Addition of PDGF to quiescent cells induced a rapid elevation of cytoplasmic free calcium concentration, [Ca2+]c, followed by a sust...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1993.2299

    authors: Mogami H,Kojima I

    更新日期:1993-10-29 00:00:00

  • High concentrations of STOP protein induce a microtubule super-stable state.

    abstract::We have previously shown that mammalian brain crude extracts contained two classes of stable microtubules: "cold stable" and "super-stable" microtubules. We now find that both species are generated by a single protein factor (STOP protein) in a dose dependent manner. These results show that STOP protein action can be ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(87)91129-6

    authors: Job D,Rauch CT,Margolis RL

    更新日期:1987-10-14 00:00:00

  • Isovalerylcarnitine is a specific activator of the high calcium requiring calpain forms.

    abstract::Isovalerylcarnitine, a product of the catabolism of L-leucine, is a potent activator of rat calpains isolated from erythrocytes, kidney, liver, skeletal and heart muscle. Only calpains II, but not calpains I, are activated by IVC, with the only exception of rat erythrocyte calpain I, the only species present in these ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(90)91775-n

    authors: Pontremoli S,Melloni E,Viotti PL,Michetti M,Di Lisa F,Siliprandi N

    更新日期:1990-02-28 00:00:00

  • Developmental expression of mRNAs from a rat C-erbA genomic locus.

    abstract::The thyroid hormone receptor alpha gene is alternatively spliced to give the alpha receptor and a variant which does not bind thyroid hormones. An additional alpha-like receptor (Rev-erbA alpha) is transcribed on the opposite strand and overlaps the unique variant sequence. The alpha receptor in rat brain reaches peak...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(92)90652-2

    authors: Jannini EA,Mitsuhashi T,Nikodem VM

    更新日期:1992-04-30 00:00:00

  • Different roles of β-arrestin and the PKA pathway in mitochondrial ROS production induced by acute β-adrenergic receptor stimulation in neonatal mouse cardiomyocytes.

    abstract::Reactive oxygen species (ROS) play a crucial role in various physiological and pathological processes mediated by β-adrenergic receptors (β-ARs) in cardiomyocytes. However, the sources and signaling pathways involved in ROS production induced by acute β-AR activation have not yet been fully defined. In primary neonata...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2017.05.140

    authors: Zhang J,Xiao H,Shen J,Wang N,Zhang Y

    更新日期:2017-08-05 00:00:00

  • Processing of atriopeptin prohormone by nonmyocytic atrial cells.

    abstract::Atriopeptin (AP) is synthesized and stored in the mammalian atria as a 126 amino acid prohormone (AP126). Upon secretion, the prohormone undergoes site specific proteolysis within the atria to yield the carboxyl terminal 28 amino acid hormone (AP28). The atrial cell responsible for AP126 bioactivation has not yet been...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(92)91105-y

    authors: Greenwald JE,Needleman P,Siegel N,Tetens E,Biel B,Ritter D

    更新日期:1992-10-30 00:00:00

  • Decrease in the T-box1 gene expression in embryonic brain and adult hippocampus of down syndrome mouse models.

    abstract::Down syndrome (DS, Trisomy 21) is the most common genetic cause of delayed fetal brain development and postnatal intellectual disability. Although delayed fetal brain development might be involved in intellectual disability, no evidence of an association between these abnormal phenotypes has been shown. To identify mo...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2020.12.026

    authors: Shimizu R,Ishihara K,Kawashita E,Sago H,Yamakawa K,Mizutani KI,Akiba S

    更新日期:2021-01-08 00:00:00

  • A novel mass spectrometric procedure to rapidly determine the position of O-acylated residues in the sequence of naturally occurring oligosaccharides.

    abstract::A novel procedure is described for the assignment of the position of O-acylated residues in the sequence of oligosaccharides. The procedure is both rapid and sensitive and complements existing carbohydrate techniques. The procedure involves the use of derivatisation, using conditions under which naturally occurring O-...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(86)91045-4

    authors: Dell A,Tiller PR

    更新日期:1986-03-28 00:00:00

  • Identity determinants of E. coli threonine tRNA.

    abstract::To investigate the identity determinants of E. coli threonine tRNA, various transcripts were prepared by in vitro transcription system with T7 RNA polymerase. Substitutions of the anticodon second letter G35 and the third letter U36 to other nucleotides led to a remarkable decrease of threonine charging activity. Char...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(92)91219-g

    authors: Hasegawa T,Miyano M,Himeno H,Sano Y,Kimura K,Shimizu M

    更新日期:1992-04-15 00:00:00

  • Control of fungal sterol C-24 transalkylation: importance to developmental regulation.

    abstract::When cultures of Gibberella fujikuroi are incubated with 24-epiiminolanosterol the introduction of a methyl group into sterol side chains at C-24 is blocked inducing a mycelial accumulation of lanosterol and 24-desalkylsterols, i.e., having the cholestane side chain. The altered sterol composition lead to aberrant myc...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(86)80006-7

    authors: Nes WD,Hanners PK,Parish EJ

    更新日期:1986-09-14 00:00:00

  • Isolation and characterization of human Nramp cDNA.

    abstract::The mouse gene locus Lsh/Ity/Bcg regulates macrophage activation for antimicrobial activity against intracellular pathogens. A candidate gene, designated natural resistance-associated macrophage protein gene (Nramp), recently isolated from a mouse pre-B cell cDNA library, encodes an integral membrane protein that has ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1994.2572

    authors: Kishi F

    更新日期:1994-11-15 00:00:00

  • Role of apoptosis in growth and differentiation of proximal tubule cells in primary cultures.

    abstract::In this study, we assessed the profile of cell proliferation, Na,K-ATPase activity and apoptosis in primary cultures of proximal tubule cells. Freshly isolated proximal tubules exhibited apoptosis very early in culture: floated cells within 6 hr; attached tubule cells within 20 hr. The onset of apoptosis in either att...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1996.0118

    authors: Tang MJ,Cheng YR,Lin HH

    更新日期:1996-01-26 00:00:00

  • Proteomic investigations of human HERC2 mutants: Insights into the pathobiology of a neurodevelopmental disorder.

    abstract::HERC2 is a giant protein with E3 ubiquitin ligase activity and other known and suspected functions. Mutations of HERC2 are implicated in the pathogenesis of various cancers and result in severe neurological conditions in Herc2-mutant mice. Recently, a pleotropic autosomal recessive HERC2-associated syndrome of intelle...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2019.02.149

    authors: Abraham JR,Barnard J,Wang H,Noritz GH,Yeganeh M,Buhas D,Natowicz MR

    更新日期:2019-04-30 00:00:00

  • TRAF-dependent association of protein kinase Tpl2/COT1 (MAP3K8) with CD40.

    abstract::Signaling by TNF-family receptor CD40 involves TRAF-family adaptor proteins, leading to activation of protein kinases that induce NFkappaB-family transcription factors. We report here that mitogen activated protein kinase kinase kinase-8 (MAP3K8), Tpl2/COT1, is recruited to the CD40 complex via a mechanism dependent o...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.12.155

    authors: Chan H,Reed JC

    更新日期:2005-03-04 00:00:00

  • MT1-MMP prevents growth inhibition by three dimensional fibronectin matrix.

    abstract::The extracellular microenvironment plays a key role in regulation of cellular functions and growth control. We show here that membrane-type 1 matrix metalloproteinase (MT1-MMP) acts as a growth promoter in confluent culture. When MT1-MMP was silenced in HT1080 fibrosarcoma cells, cells created three dimensional (3D) f...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2013.05.134

    authors: Takino T,Guo L,Domoto T,Sato H

    更新日期:2013-07-05 00:00:00