Abstract：
:Phosphorylation of regulatory light chain (LC20) by myosin light chain kinase (MLCK) has been thought to play an important role in both smooth muscle contraction and several functions of vertebrate non-muscle cells. Amiloride, a frequently used Na+/H+ exchange inhibitor, potently inhibited phosphorylation of LC20 by MLCK. The inhibition was non-competitive with respect to myosin but competitive with ATP (Ki = 0.95 microM), suggesting that amiloride may act as an ATP analogue. Amiloride also inhibited the tension development of ether-treated gizzard fibers which were lacking in Na+/H+ antiport, even in the presence of ATP regenerating system. Thus, it must be reminded that amiloride cannot be used as a specific inhibitor of Na+/H+ exchange, and that the inhibition of myosin phosphorylation by amiloride should be taken into consideration in studying the role of Na+/H+ antiport in the cellular function.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Higashihara Mdoi
10.1016/0006-291x(89)90808-5subject
Has Abstractpub_date
1989-08-15 00:00:00pages
1253-9issue
3eissn
0006-291Xissn
1090-2104pii
0006-291X(89)90808-5journal_volume
162pub_type
杂志文章abstract：:Type 1 diabetes (T1D) is an autoimmune disease caused by destruction of insulin-producing β cells. The response of autoreactive T cells to β cell antigens plays a central role in the development of T1D. Recently, fusion peptides composed by insulin C-peptide fragments and other proteins were reported as β cell target ...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2020.11.019
更新日期：2021-01-01 00:00:00
abstract：:Hippo pathway regulates cell proliferation and differentiation. Yes-associated protein (Yap) and transcriptional coactivator with PDZ-binding motif (Taz) are effectors of Hippo pathway. The function of Yap/Taz in embryonic liver development has yet to be reported. Here yap1 and taz were found expressed in liver and ot...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2018.05.196
更新日期：2018-09-03 00:00:00
abstract：:GPR35, previously an orphan G-protein coupled receptor, is a receptor for kynurenic acid. Here we examine the distribution of GPR35 in the rat dorsal root ganglion (DRG) and the effects of its selective activation. GPR35 was expressed predominantly by small- to medium-diameter neurons of the DRG. Many of these same ne...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2007.10.197
更新日期：2008-01-11 00:00:00
abstract：:Laminin components were solubilized from basement membranes of amnion, chorion and chorionic microvessels of human placenta without prior protease digestion. These structures, after isolation, were initially processed in a sonicator bath containing a solution of Triton X-100, EDTA and 2M NaCl and the laminins extracte...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/0006-291x(83)91730-8
更新日期：1983-05-16 00:00:00
abstract：:Of two major isoforms of angiotensin II receptors, AT1 and AT2, biological roles of AT2 remain unclear. Using vascular smooth muscle cells, we investigated the regulation of expression of AT2 by growth factors in comparison with that of AT1. The cultured rat aorta smooth muscle cells had detectable AT2 binding sites, ...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1993.1844
更新日期：1993-07-15 00:00:00
abstract：:Streptavidin, which is one of the most widely used proteins in biotechnological application field and is active only in tetrameric form, was surface expressed on the surface of Bacillus subtilis spore. Spore coat protein of B. subtilis, CotG, was used as an anchoring motif to display streptavidin. FACS using anti-stre...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2005.03.144
更新日期：2005-05-27 00:00:00
abstract：:We have extracted and purified proteins from calcium containing urinary stones with 4 M guanidine chloride and sequenced the amino acids of the proteins. The protein of approximately 49kDa was defined as human alpha-one antitrypsin (AT, from 23rd to 42nd amino acids of NH2-terminal) by amino acid sequence analysis. Th...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1993.1731
更新日期：1993-06-30 00:00:00
abstract：:The role of mycothiol in mycobacteria was examined by comparative analysis of mutants disrupted in the four known genes encoding the protein machinery needed for mycothiol biosynthesis. These mutants were sensitive to acid stress, antibiotic stress, alkylating stress, and oxidative stress indicating that mycothiol and...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2007.08.142
更新日期：2007-11-09 00:00:00
abstract：:The question of how eukaryotic cells assemble their mitochondria was long considered to be inaccessible to biochemical investigation. This attitude changed about fifty years ago when the powerful tools of yeast genetics, electron microscopy and molecular biology were brought to bear on this problem. The rising interes...
journal_title：Biochemical and biophysical research communications
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.bbrc.2013.03.081
更新日期：2013-05-10 00:00:00
abstract：:While there have been many single-molecule studies of kinesin-1, most have been done along microtubules purified from bovine or porcine brain, and relatively little is known about how variations in tubulin might alter motor function. Of particular interest is transport along microtubules polymerized from tubulin purif...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2014.10.119
更新日期：2014-11-28 00:00:00
abstract：:High serum selenium levels have been associated epidemiologically with increased incidence of type 2 diabetes. The major fraction of total selenium in serum is represented by liver-derived selenoprotein P (SeP). This study was undertaken to test for a hypothesized effect of hyperglycemia and the antihyperglycemic drug...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2009.06.143
更新日期：2009-09-11 00:00:00
abstract：:Arrhythmogenic right ventricular dysplasia/cardiomyopathy type 2 (ARVD2, OMIM 600996) and stress-induced polymorphic ventricular tachycardia (VTSIP, OMIM 604772) are two cardiac diseases causing juvenile sudden death, both associated with mutations in the RyR2 calcium channel. By using a quantitative yeast two-hybrid ...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/s0006-291x(02)02689-x
更新日期：2002-12-13 00:00:00
abstract：:A cDNA for murine transcription factor S-II (Hirashima et al., J. Biol. Chem (1988) 263, 3858-3863) was inserted into a silkworm baculovirus vector and expressed in Bm-N cells, a cell line of Bombyx mori maintained in the laboratory. Recombinant S-II was purified from virus-infected cell extracts to near homogeneity b...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/s0006-291x(89)80115-9
更新日期：1989-05-15 00:00:00
abstract：:Cellular prion protein, PrP(C), is essential for the development of prion diseases where it is considered to be a substrate for the formation of the disease-associated conformer, PrP(Sc). In sheep, PrP(C) is abundant in neuronal tissue and is also found at lower concentrations in a range of non-neuronal tissues, inclu...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2006.12.006
更新日期：2007-02-02 00:00:00
abstract：:alpha-Crystallin, a major eye lens protein, has been shown to function like a molecular chaperone by suppressing the aggregation of other proteins induced by various stress conditions. Ultraviolet (UV) radiation is known to cause structural and functional alterations in the lens macromolecules. Earlier we observed tha...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.2001.4642
更新日期：2001-04-06 00:00:00
abstract：:The minichromosome maintenance (MCM) proteins are essential for DNA replication initiation and elongation in eukaryotes. In mammalian cells, MCM2-MCM7 complexes are believed to unwind DNA during chromosomal DNA replication. Here we identified a novel MCM family gene, MCM9, by using bioinformatics. Human, mouse, and ra...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2005.03.222
更新日期：2005-06-03 00:00:00
abstract：:Curcumin, a component of turmeric (Curcuma longa), has been reported to suppress beta-catenin response transcription (CRT), which is aberrantly activated in colorectal cancer. However, the effects of its natural analogs (demethoxycurcumin [DMC] and bisdemethoxycurcumin [BDMC]) and metabolite (tetrahydrocurcumin [THC])...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2008.10.171
更新日期：2008-12-26 00:00:00
abstract：:Statins confer therapeutic benefits in systemic and pulmonary vascular diseases. Bone morphogenetic protein (BMP) receptors serve essential signaling functions in cardiovascular development and skeletal morphogenesis. Mutations in BMP receptor type II (BMPR2) are associated with human familial and idiopathic pulmonary...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2005.10.187
更新日期：2006-01-06 00:00:00
abstract：:PhyA gene products of Aspergillus ficuum (AF) and Peniophora lycii (PL) as expressed in industrial strains of Aspergillus niger and Aspergillus oryzae, respectively, were purified to homogeneity and then characterized for both physical and biochemical properties. The PL phytase is 26 amino acid residues shorter than t...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/s0006-291x(03)00374-7
更新日期：2003-04-04 00:00:00
abstract：:Myocyte enhancer factor 2C (MEF2C) plays an important role in cardiovascular development and is a key transcription factor for cardiac hypertrophy. Here, we describe MEF2C regulation by insulin-like growth factor-1 (IGF-1) and its role in IGF-1-induced cardiac hypertrophy. We found that IGF-1 addition to cultured rat ...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2009.07.147
更新日期：2009-10-09 00:00:00
abstract：:We investigated the possible involvement of neuromedin U (NMU) and neuromedin S (NMS) in thermoregulation in rats. Intracerebroventricular (icv) injection of NMU or NMS increased the back surface temperature (BS-T) in a dose-dependent manner during both the light and dark periods. Pre-treatment with the β3 blocker SR5...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2016.01.155
更新日期：2016-02-19 00:00:00
abstract：:Leucine-rich repeat kinase 2 (LRRK2) is the causal molecule of familial Parkinson's disease (PD), but its true physiological function remains unknown. In the normal mouse, LRRK2 is expressed in kidney, spleen, and lung at much higher levels than in brain, suggesting that LRRK2 may play an important role in these organ...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2010.01.041
更新日期：2010-02-12 00:00:00
abstract：:Several lines of evidence provided by other workers indicate that within the same species thyroid hormone binding protein, the beta-subunit of prolyl hydroxylase, and protein disulfide isomerase are the same protein. We sought to determine if glycosylation site binding protein, a lumenal protein of the endoplasmic ret...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/0006-291x(90)90538-x
更新日期：1990-08-16 00:00:00
abstract：:We have previously shown that mammalian brain crude extracts contained two classes of stable microtubules: "cold stable" and "super-stable" microtubules. We now find that both species are generated by a single protein factor (STOP protein) in a dose dependent manner. These results show that STOP protein action can be ...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/0006-291x(87)91129-6
更新日期：1987-10-14 00:00:00
abstract：:Low voltage-activated, rapidly inactivating T-type Ca(2+) channels are found in a variety of cells where they regulate electrical activity and Ca(2+) entry. In whole-cell patch clamp recordings from bovine adrenal zona fasciculata cells, cis-polyunsaturated omega-3 fatty acids including docosahexaenoic acid (DHA), eic...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2004.12.033
更新日期：2005-02-11 00:00:00
abstract：:Genetic predisposition is necessary for polycystic kidney disease (PKD) initiation, although there are other, incompletely identified downstream processes that are required for cyst growth. Their characterization may provide a unique opportunity for clinical interventions. One of the poorly studied phenomena in PKD is...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2019.03.177
更新日期：2019-05-21 00:00:00
abstract：:In the present paper we report evidence about the formation of polyprenyl-phosphate monosaccharides, their elongation products and the assembly of dolichyl-diphosphate-oligosaccharide to endogenous T47D clone 11 proteins upon incubation with [14C]glucose. The influence of estradiol and two nonesteroidal antiestrogens ...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/0006-291x(85)90617-5
更新日期：1985-01-16 00:00:00
abstract：:Abnormal plasma ascorbic acid (AA) and dehydroascorbic acid (DHAA) levels observed in diabetes may be correlated to a deficiency in the recycling of AA. Ascorbic acid and DHAA levels are altered in diabetic liver in the present study. In addition, a coupling of the hexose monophosphate (HMP) shunt by way of NADPH to g...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1993.1365
更新日期：1993-03-31 00:00:00
abstract：:The effects of graded doses (5 x 10(-8) to 10(-5)) acetylcholine on intracellular Ca2+ and on 86Rb and 36Cl efflux were compared in submandibular cell clusters of 1 and 7 day-old and adult rats. Initial Ca2+ peaks were similar at agonists concentrations lower than 10(-7) M but the release of Rb+ and Cl- were smaller i...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/0006-291x(92)91353-r
更新日期：1992-11-16 00:00:00
abstract：:We present here the biosynthesis of AgNps from the aqueous extract of H. thebaica fruit, and monitored through UV-Vis spectrophotometer. The functional group were characterized through ATR-FTIR spectroscopy, the particle size, morphologies and elemental composition of the nanoparticles were investigated by using TEM, ...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2017.06.136
更新日期：2017-08-26 00:00:00
