Abstract:
:Streptavidin, which is one of the most widely used proteins in biotechnological application field and is active only in tetrameric form, was surface expressed on the surface of Bacillus subtilis spore. Spore coat protein of B. subtilis, CotG, was used as an anchoring motif to display streptavidin. FACS using anti-streptavidin antibody was used for the verification of surface localization of expressed CotG-streptavidin fusion protein. FACS and dot-blot were used for the verification of biological activity of displayed streptavidin with FITC-labeled biotin.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Kim JH,Lee CS,Kim BGdoi
10.1016/j.bbrc.2005.03.144subject
Has Abstractpub_date
2005-05-27 00:00:00pages
210-4issue
1eissn
0006-291Xissn
1090-2104pii
S0006-291X(05)00611-Xjournal_volume
331pub_type
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