Spore-displayed streptavidin: a live diagnostic tool in biotechnology.

Abstract:

:Streptavidin, which is one of the most widely used proteins in biotechnological application field and is active only in tetrameric form, was surface expressed on the surface of Bacillus subtilis spore. Spore coat protein of B. subtilis, CotG, was used as an anchoring motif to display streptavidin. FACS using anti-streptavidin antibody was used for the verification of surface localization of expressed CotG-streptavidin fusion protein. FACS and dot-blot were used for the verification of biological activity of displayed streptavidin with FITC-labeled biotin.

authors

Kim JH,Lee CS,Kim BG

doi

10.1016/j.bbrc.2005.03.144

subject

Has Abstract

pub_date

2005-05-27 00:00:00

pages

210-4

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(05)00611-X

journal_volume

331

pub_type

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