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Kinesin-1 translocation: Surprising differences between bovine brain and MCF7-derived microtubules.

Abstract：

:While there have been many single-molecule studies of kinesin-1, most have been done along microtubules purified from bovine or porcine brain, and relatively little is known about how variations in tubulin might alter motor function. Of particular interest is transport along microtubules polymerized from tubulin purified from MCF7 breast cancer cells, both because these cells are a heavily studied model system to help understand breast cancer, and also because the microtubules are already established to have interesting polymerization/stability differences from bovine tubulin, suggesting that perhaps transport along them is also different. Thus, we carried out paired experiments to allow direct comparison of in vitro kinesin-1 translocation along microtubules polymerized from either human breast cancer cells (MCF7) or microtubules from bovine brain. We found surprising differences: on MCF7 microtubules, kinesin-1's processivity is significantly reduced, although its velocity is only slightly altered.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Feizabadi MS,Jun Y

doi

10.1016/j.bbrc.2014.10.119

subject

Has Abstract

pub_date

2014-11-28 00:00:00

pages

543-6

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(14)01939-1

journal_volume

454

pub_type

杂志文章

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