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Production of functional S-II in Bombyx mori cells.

Abstract：

:A cDNA for murine transcription factor S-II (Hirashima et al., J. Biol. Chem (1988) 263, 3858-3863) was inserted into a silkworm baculovirus vector and expressed in Bm-N cells, a cell line of Bombyx mori maintained in the laboratory. Recombinant S-II was purified from virus-infected cell extracts to near homogeneity by ammonium sulfate fractionation, and chromatographies on DEAE-cellulose and phosphocellulose. About 60 micrograms of recombinant S-II was obtained from 1 g of virus-infected cells. The molecular mass and specific activity of recombinant S-II were exactly the same as those of authentic S-II purified from Ehrlich ascites tumor cells.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Hirashima S,Horikoshi N,Sekimizu K,Natori S

doi

10.1016/s0006-291x(89)80115-9

subject

Has Abstract

pub_date

1989-05-15 00:00:00

pages

1093-9

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(89)80115-9

journal_volume

160

pub_type

杂志文章

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