当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Molecular and functional characterization of a spermidine transporter (TcPAT12) from Trypanosoma cruzi.

Molecular and functional characterization of a spermidine transporter (TcPAT12) from Trypanosoma cruzi.

Abstract：

:Trypanosoma cruzi, the etiological agent of Chagas' disease, is the only eukaryotic cell which lacks the ability to synthesize polyamines de novo. In this work, we describe for the first time the molecular and biochemical properties of a high-affinity spermidine transporter from T. cruzi. The transporter gene TcPAT12 was functionally expressed in Xenopus laevis oocytes, showing high levels of spermidine uptake. Similar apparent affinity constants for spermidine uptake were obtained when comparing T. cruzi epimastigotes and heterologous expressed TcPAT12 in X. laevis. In addition, TcPAT12 also transports putrescine and the amino acid l-arginine at lower rates than spermidine.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Carrillo C,Canepa GE,Algranati ID,Pereira CA

doi

10.1016/j.bbrc.2006.03.215

subject

Has Abstract

pub_date

2006-06-09 00:00:00

pages

936-40

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(06)00799-6

journal_volume

344

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Rnf112 deletion protects brain against intracerebral hemorrhage (ICH) in mice by inhibiting TLR-4/NF-κB pathway.

    abstract：:Intracerebral hemorrhage (ICH) is reported as a common and often fatal type of stroke accompanied with high morbidity and mortality, and it frequently results in long-lasting neurological dysfunctions. However, the pathogenesis that contributes to ICH has not been fully understood. Rnf112, also known as Znf179, is a m...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.10.141

    authors： Zhang F,Zhang C

    更新日期：2018-12-09 00:00:00

  • Evidence for the presence of DNA primase in mitochondria of Saccharomyces cerevisiae.

    abstract：:Chromatography of a DNA polymerase preparation from mitochondria of Saccharomyces cerevisiae on DNA-cellulose column, using Tris-HCl (pH 7.5) buffer containing 0.6 M NaCl as eluent, was found to yield a fraction exhibiting DNA primase-like activity free of DNA polymerase. This fraction could support the synthesis of 1...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92464-9

    authors： Desai SD,Pasupathy K,Chetty KG,Pradhan DS

    更新日期：1989-04-28 00:00:00

  • Expression of tenascin in the developing human small intestine.

    abstract：:The expression of tenascin (Tn), an extracellular matrix glycoprotein, was analyzed in the human small intestine. Three major oligomeric forms of intestinal Tn (320 kDa, 220 kDa and 200 kDa) were identified in both the fetus and the adult. The intestinal 200 kDa form was not found expressed by intestinal mesenchymal c...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1528

    authors： Beaulieu JF,Jutras S,Kusakabe M,Perreault N

    更新日期：1993-05-14 00:00:00

  • High- and low-affinity CCKA receptor states mediate specific growth inhibitory effects on CHO cells.

    abstract：:To relate specific effects on growth and transformation to activation of specific affinity states of the CCKA receptor stably expressed in CHO cells we compared responses to the CCK analogues JMV-180 and CCK8. CCK8 led to an inhibition of both cell proliferation and transformation. Effects on proliferation were indica...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2096

    authors： Detjen K,Tseng MJ,Logsdon CD

    更新日期：1995-08-04 00:00:00

  • Down-regulation of TRAF4 targeting RSK4 inhibits proliferation, invasion and metastasis in breast cancer xenografts.

    abstract：:Ribosomal S6 protein kinase 4 (RSK4) was known as a novel tumor suppressor gene, and the tumor necrosis factor receptor-associated factor 4 (TRAF4) was linked to carcinogenesis. The purpose of this study is to further investigate the effect of the TRAF4 gene on cell proliferation, invasion and metastasis in vivo and e...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.04.164

    authors： Zhu L,Zhang S,Huan X,Mei Y,Yang H

    更新日期：2018-06-07 00:00:00

  • Role of VLDL/chylomicron in amyloid formation in familial amyloidotic polyneuropathy.

    abstract：:We examined the affinity of transthyretin (TTR) for lipoproteins and the effect of lipoproteins on TTR-related amyloidogenesis using serum samples from healthy volunteers and patients with familial amyloidotic polyneuropathy (FAP) ATTRVal30Met. In both volunteers and patients, TTR levels were highest in the VLDL fract...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.10.001

    authors： Sun X,Ando Y,Haraoka K,Katsuragi S,Yamashita T,Yamashita S,Okajima M,Terazaki H,Okabe H

    更新日期：2003-11-14 00:00:00

  • MAEBL Plasmodium falciparum protein peptides bind specifically to erythrocytes and inhibit in vitro merozoite invasion.

    abstract：:MAEBL is an erythrocyte binding protein located in the rhoptries and on the surface of mature merozoites, being expressed at the beginning of schizogony. The structure of MAEBL originally isolated from rodent malaria parasites suggested a molecule likely to be involved in invasion. We thus became interested in identif...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.01.050

    authors： Ocampo M,Curtidor H,Vera R,Valbuena JJ,Rodríguez LE,Puentes A,López R,García JE,Tovar D,Pacheco P,Navarro MA,Patarroyo ME

    更新日期：2004-03-05 00:00:00

  • Oxidative stress potentially enhances FcεRI-mediated leukotriene C4 release dependent on the late-phase increase of intracellular glutathione in mast cells.

    abstract：:Cysteinyl leukotrienes (cysLTs), which include leukotriene C4 (LTC4), are the predominant class of LTs synthesized by mast cells. CysLTs can induce many of the abnormalities seen in asthma. LTC4 is generated by the conjugation of LTA4 with reduced glutathione (GSH) by LTC4 synthase. During screening of the effects of ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.08.081

    authors： Seki K,Hisada T,Kawata T,Kamide Y,Dobashi K,Yamada M,Mori M,Okajima F,Ishizuka T

    更新日期：2013-09-27 00:00:00

  • Long noncoding RNA CDKN2B-AS1 interacts with miR-411-3p to regulate ovarian cancer in vitro and in vivo through HIF-1a/VEGF/P38 pathway.

    abstract：:Ovarian cancer (OC) is one of the most prevalent cancers with high fatality rate. In the present study, RT-PCR showed that the mRNA level of CDKN2B-AS1 was significantly upregulated while the miR-411-3p was downregulated in OC cell lines. In addition, the Sh-CDKN2B-AS1 resulted in the suppression of cell growth, invas...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.03.141

    authors： Wang Y,Huang Y,Liu H,Su D,Luo F,Zhou F

    更新日期：2019-06-18 00:00:00

  • Cleavage of recombinant enkephalin precursor by endoproteolytic activity in bovine chromaffin granules.

    abstract：:To identify endoproteolytic activity that processes the enkephalin precursor, a novel approach was undertaken for the production of model substrate in the form of recombinant 35S-(Met)-preproenkephalin (35S-(Met)-PPE), generated by in vitro transcription and translation of the rat PPE cDNA. Endoproteolytic activity in...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)92085-e

    authors： Hook VY,Hegerle D,Affolter HU

    更新日期：1990-03-16 00:00:00

  • Molecular cloning of Ebitein1: a novel extracellular signal-regulated kinase 2-binding protein in testis.

    abstract：:We cloned a cDNA encoding a novel extracellular signal-regulated kinase (ERK) 2-binding protein, EBITEIN1, by yeast two-hybrid screening. Northern and Western blotting experiments showed that the transcript and protein were expressed in the testes. Furthermore, immunohistochemical experiments showed that EBITEIN1 exis...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.01.078

    authors： Miura K,Imaki J

    更新日期：2008-04-04 00:00:00

  • Retracted: Apoptosis of CT26 colorectal cancer cells induced by Clostridium difficile toxin A stimulates potent anti-tumor immunity.

    abstract：:Clostridium difficile toxin A (TcdA) is one of the main pathogenic factors released by C. difficile. Due to its potent cytotoxic and proinflammatory activities, we investigated the anti-tumor activity of TcdA. CT26 colorectal cancer cells were challenged with recombinant TcdA, and it was found that TcdA could induce a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,收录出版

    doi：10.1016/j.bbrc.2012.04.068

    authors： Tian Y,Huang T,Li G,Liu J,Wang X,Feng H,Wang J

    更新日期：2012-05-25 00:00:00

  • Stimulatory effect of insulin on renal proximal tubule sodium transport is preserved in type 2 diabetes with nephropathy.

    abstract：:Our previous study indicates that hyperinsulinemia in metabolic syndrome in the absence of nephropathy may promote hypertension by stimulating renal proximal tubule (PT) sodium transport via insulin receptor substrate (IRS) 2/phosphoinositide 3-kinase pathway. In the present study we showed that the stimulatory effect...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.04.005

    authors： Nakamura M,Satoh N,Suzuki M,Kume H,Homma Y,Seki G,Horita S

    更新日期：2015-05-22 00:00:00

  • Co-crystal structures of PTK6: With Dasatinib at 2.24 Å, with novel imidazo[1,2-a]pyrazin-8-amine derivative inhibitor at 1.70 Å resolution.

    abstract：:Human Protein tyrosine kinase 6 (PTK6)(EC:2.7.10.2), also known as the breast tumor kinase (BRK), is an intracellular non-receptor Src-related tyrosine kinase expressed five-fold or more in human breast tumors and breast cancer cell lines but its expression being low or completely absent from normal mammary gland. The...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.12.030

    authors： Thakur MK,Birudukota S,Swaminathan S,Battula SK,Vadivelu S,Tyagi R,Gosu R

    更新日期：2017-01-22 00:00:00

  • Post-translational self-hydroxylation: a probe for oxygen activation mechanisms in non-heme iron enzymes.

    abstract：:Recent years have seen considerable evolution in our understanding of the mechanisms of oxygen activation by non-heme iron enzymes, with high-valent iron-oxo intermediates coming to the forefront as formidably potent oxidants. In the absence of substrate, the generation of vividly colored chromophores deriving from th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.08.191

    authors： Farquhar ER,Koehntop KD,Emerson JP,Que L Jr

    更新日期：2005-12-09 00:00:00

  • Glucose-stimulated efflux of fura-2 in pancreatic beta-cells is prevented by probenecid.

    abstract：:Fura-2 loaded pancreatic beta-cells, isolated from obese hyperglycemic mice, were studied with respect to cytoplasmic free Ca2+ concentration ([Ca2+]i), insulin release and efflux of indicator. In the absence of glucose there was a continuous efflux of fura-2, which was markedly increased by stimulation with a high co...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92426-1

    authors： Arkhammar P,Nilsson T,Berggren PO

    更新日期：1989-02-28 00:00:00

  • Isolation of DNA-free RNA, DNA, and proteins by cesium trifluoroacetate centrifugation.

    abstract：:The ability to simultaneously isolate intact DNA-free RNA, genomic DNA, and proteins from a biological specimen can be useful in cloning genes and analyzing gene expression. Equilibrium density gradient centrifugation with CsCl is a useful tool for fractionating, quantitatively separating, and characterizing RNA, DNA,...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.09.246

    authors： Zhang H,Chen HT,Glisin V

    更新日期：2003-12-05 00:00:00

  • Effects of hydrogen sulfide on homocysteine-induced oxidative stress in vascular smooth muscle cells.

    abstract：:Hydrogen sulfide (H(2)S) is an important gasotransmitter that generated in mammalian cells from l-cysteine metabolism. Little is known about its protective role in oxidative stress. In the present study, we investigated whether H(2)S could affect homocysteine (HCY)-induced cytotoxicity and oxidative stress in vascular...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.10.058

    authors： Yan SK,Chang T,Wang H,Wu L,Wang R,Meng QH

    更新日期：2006-12-15 00:00:00

  • CCAAT/enhancer-binding protein CEBP-2 controls fat consumption and fatty acid desaturation in Caenorhabditis elegans.

    abstract：:Mammalian CCAAT/enhancer-binding proteins (C/EBPs) are generally known as regulators in adipocyte differentiation. However, more understanding of the role of C/EBPs in lipid and glucose metabolism remains to be discovered. In this study, we verified the effect of CEBP-2, the homolog of CEBPs, on fat storage in Caenorh...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.10.106

    authors： Xu XY,Hu JP,Wu MM,Wang LS,Fang NY

    更新日期：2015-12-04 00:00:00

  • A small compound targeting the interaction between nonstructural proteins 2B and 3 inhibits dengue virus replication.

    abstract：:The non-structural protein NS2B/NS3 serine-protease complex of the dengue virus (DENV) is required for the maturation of the viral polyprotein. Dissociation of the NS2B cofactor from NS3 diminishes the enzymatic activity of the complex. In this study, we identified a small molecule inhibitor that interferes with the i...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.09.078

    authors： Pambudi S,Kawashita N,Phanthanawiboon S,Omokoko MD,Masrinoul P,Yamashita A,Limkittikul K,Yasunaga T,Takagi T,Ikuta K,Kurosu T

    更新日期：2013-10-25 00:00:00

  • Kinetics of fluid-phase pinocytosis in Dictyostelium discoideum amoebae.

    abstract：:Kinetics of pinocytosis in Dictyostelium discoideum were investigated over an extended period of time (up to 6 hours) using fluorescein isothiocyanate (FITC)-dextran as a fluid-phase marker. FITC-dextran added to the medium accumulated rapidly inside the cells with a rate of influx equivalent to 9 microns3 of fluid/ce...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(86)80402-8

    authors： Klein G,Satre M

    更新日期：1986-08-14 00:00:00

  • Glycosylated human growth hormone (hGH): a novel 24 kDa hGH-N variant.

    abstract：:We have identified a human pituitary protein as a novel glycosylated variant of hGH. Isolation of the denatured protein included separation of human pituitary extract by Sephadex G-100 chromatography in ammonium bicarbonate, followed by Sephadex G-100 chromatography in 10% acetic acid, with subsequent DEAE Sephacryl c...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1697

    authors： Haro LS,Lewis UJ,Garcia M,Bustamante J,Martinez AO,Ling NC

    更新日期：1996-11-12 00:00:00

  • Fission yeast Mcl1 interacts with SCF(Pof3) and is required for centromere formation.

    abstract：:The fission yeast S-phase regulator Mcl1, an orthologue of budding yeast Ctf4, is an interacting protein of DNA polymerase alpha and an important factor to ensure DNA replication and sister chromatid cohesion. Deletion of this protein results in severe cohesion defects, however, the function and cellular role of this ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.09.024

    authors： Mamnun YM,Katayama S,Toda T

    更新日期：2006-11-10 00:00:00

  • Crystal structure of BrlR with c-di-GMP.

    abstract：:The transcriptional regulator BrlR is a member of the MerR family of multidrug transport activators in Pseudomonas aeruginosa. Recent study indicates that BrlR is a novel 3',5'-cyclic diguanylic acid (c-di-GMP) receptor and can be activated by c-di-GMP. To gain insight into BrlR function, we determined the structure o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.06.033

    authors： Raju H,Sharma R

    更新日期：2017-08-19 00:00:00

  • In vitro polymerization of Mycobacterium leprae FtsZ OR Mycobacterium tuberculosis FtsZ is revived or abolished, respectively, by reciprocal mutation of a single residue.

    abstract：:A single residue that dramatically influences polymerization of principal cell division protein FtsZ of Mycobacterium leprae (MlFtsZ) and Mycobacterium tuberculosis (MtFtsZ) has been identified. Soluble, recombinant MlFtsZ did not show polymerization in vitro, in contrast to MtFtsZ, which polymerised. Mutation of the ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.01.115

    authors： Gupta P,Srinivasan R,Rajeswari H,Indi S,Ajitkumar P

    更新日期：2008-04-04 00:00:00

  • Graphene coating on the surface of CoCrMo alloy enhances the adhesion and proliferation of bone marrow mesenchymal stem cells.

    abstract：:The objective was to investigate whether a graphene coating could improve the surface bioactivity of a cobalt-chromium-molybdenum-based alloy (CoCrMo). Graphene was produced by chemical vapor deposition and transferred to the surface of the CoCrMo alloy using an improved wet transfer approach. The morphology of the sa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.02.152

    authors： Zhang Q,Li K,Yan J,Wang Z,Wu Q,Bi L,Yang M,Han Y

    更新日期：2018-03-18 00:00:00

  • Site-directed mutation on the only universally conserved residue Leu122 of small heat shock protein Hsp16.3.

    abstract：:Hsp16.3 from Mycobacterium tuberculosis belongs to the small heat shock protein family and has chaperone-like activity in vitro. The only universally conserved hydrophobic residue Leu122 was substituted by Val and Ala, respectively. The mutations on the Leu122 of Hsp16.3 have resulted in much lower structural stabilit...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.6062

    authors： Mao Q,Chang Z

    更新日期：2001-12-21 00:00:00

  • Electrospray ionization mass spectrometry: a promising new technique in the study of protein/DNA noncovalent complexes.

    abstract：:With the emergence of electrospray ionization mass spectrometry (ESI-MS), mass spectrometry is no longer restricted to the study of small, stable molecules, but has become a viable technique to study large biomolecules as well as noncovalent biomolecular complexes. ESI-MS has been used to study noncovalent interaction...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1006/bbrc.1998.0103

    authors： Veenstra TD

    更新日期：1999-04-02 00:00:00

  • Increased free-cystine content of fibroblasts cultured from patients with cystinosis.

    abstract：:The presence of a significantly increased content of free-cystine in skin fibroblasts from both homozygotes and heterozygotes for cystinosis emphasizes the central role of cystine in this disease, even though the primary defect responsible for cystine accumulation is yet to be determined. The studies described in this...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(67)90516-5

    authors： Schneider JA,Rosenbloom FM,Bradley KH,Seegmiller JE

    更新日期：1967-11-30 00:00:00

  • Restoration and stimulation of the in vitro immune response of B cells to sheep erythrocytes by interleukins and muramyl dipeptide (MDP).

    abstract：:MDP, a synthetic muramyl dipeptide, is capable of increasing the primary in vitro antibody response, to sheep erythrocytes, of purified B cells restored with a monokine and helper T cell factors, including Interleukin 2 and the late-acting T cell replacing factor (TRF). First, the possibility that the adjuvanticity of...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(84)80386-1

    authors： Souvannavong V,Adam A

    更新日期：1984-11-30 00:00:00