Evidence for alternative RNA splicing and possible alternative promoters in the human muscle phosphofructokinase gene at the 5' untranslated region.

abstract：:A baculovirus expression system has been used to express large quantities of full-length, biologically active Xenopus fibroblast growth factor receptor-1. Recombinant XFGFR-1 was purified to near homogeneity by a single step immunoaffinity purification procedure. The recombinant XFGFR-1 binds both FGF-1 and FGF-2 with...

journal_title：Biochemical and biophysical research communications

authors： Brown SA,Friesel R

更新日期：1993-06-30 00:00:00

abstract：:A series of novel, azacyclic ureas which are highly potent inhibitors of the HIV-1 protease (IC50 = 4.1 to < 0.5 nM) were synthesized. Aqueous solubilities of this series of compounds were improved by incorporating polar functional groups at the P1' P2 and P2' positions. These compounds also possess good anti-viral ac...

journal_title：Biochemical and biophysical research communications

authors： Sham HL,Zhao C,Marsh KC,Betebenner DA,Lin S,Rosenbrook W Jr,Herrin T,Li L,Madigan D,Vasavanonda S,Molla A,Saldivar A,McDonald E,Wideburg NE,Kempf D,Norbeck DW,Plattner JJ

更新日期：1996-08-14 00:00:00

abstract：:Calcineurin Inhibitors (CNIs) are routinely used for immunosuppression following solid organ transplantation. However, the prolonged use of these agents lead to organ fibrosis which limits their efficacy. CNIs induce TGFβ expression, which is reported to augment endothelial-to-mesenchymal transition (EndMT), but their...

journal_title：Biochemical and biophysical research communications

authors： Woda CB,Bruneau S,Mak AL,Haskova Z,Liu K,Ghosh CC,Briscoe DM

更新日期：2019-11-19 00:00:00

abstract：:A protein of Mr 16,000 (protein 16 K) which tightly binds to DNA has been isolated from E. coli. The amino acid composition and amino-terminal amino acid sequence of this protein have been determined. On the basis of its physico-chemical characteristics the protein 16 K was shown to be identical to the protein H1 [Cuk...

journal_title：Biochemical and biophysical research communications

authors： Laine B,Sautiere P,Spassky A,Rimsky S

更新日期：1984-03-30 00:00:00

abstract：:Cytochrome P450s (CYPs) play critical role in oxidative metabolism of numerous xenobiotics and endogenous compounds. The first CYP3A subfamily member in saltwater crocodile has been cloned and modelled for three-dimensional (3D) structure. The full-length cDNA was obtained employing reverse transcription polymerase ch...

journal_title：Biochemical and biophysical research communications

authors： Tabassum R

更新日期：2019-08-27 00:00:00

abstract：:Lon protease has been well studied in many aspects; however, the DNA-binding specificity of Lon in prokaryotes has not been clearly identified. Here we examined the DNA-binding activity of Lon protease alpha-domains from Brevibacillus thermoruber (Bt), Bacillus subtilis (Bs), and Escherichia coli (Ec). MALDI-TOF mass ...

journal_title：Biochemical and biophysical research communications

authors： Lin YC,Lee HC,Wang I,Hsu CH,Liao JH,Lee AY,Chen C,Wu SH

更新日期：2009-10-09 00:00:00

abstract：:Under standard conditions, in the presence of GTP, highly purified eIF-2 and Co-eIF-2 factor preparations efficiently stimulated AUG-codon dependent but not physiological mRNA-dependent Met-tRNAf binding to 40S ribosomes. Replacement of GTP by a nonhydrolyzable GTP analog, GMP-PNP, in the above system, gave significan...

journal_title：Biochemical and biophysical research communications

authors： Roy R,Nasrin N,Ahmad MF,Gupta NK

更新日期：1984-08-16 00:00:00

abstract：:Heat stress results in the cellular accumulation of heat-shock proteins (HSP) and increased protein glycosylation. Among known stress glycoproteins, GP50 and GP62 are associated with the expression of thermotolerance. In the present study, we characterized subcellular localization and redistribution of GP62 and GP50 i...

journal_title：Biochemical and biophysical research communications

authors： Jethmalani SM,Henle KJ

更新日期：1997-08-18 00:00:00

abstract：:The reaction of the crosslinker dibromobimane has recently revealed a functionally important internal loop structure within the skeletal myosin S1 heavy chain where Cys-522 of the 50K domain and Cys-707 (SH1) of the 20K region are spatially juxtaposed. Here we have studied the possible relevance of this topological fe...

journal_title：Biochemical and biophysical research communications

authors： Bonet A,Audemard E,Mornet D

更新日期：1988-04-15 00:00:00

abstract：:Catabolite inactivation of fructose 1,6-bisphosphatase and cytoplasmic malate dehydrogenase was studied using the protease-deficient and vacuole-defective yeast strain pep4-3. The catabolite inactivation of fructose 1,6-bisphosphatase in pep4-3 was found to have a normal first inactivation step but with a defective se...

journal_title：Biochemical and biophysical research communications

authors： Funaguma T,Toyoda Y,Sy J

更新日期：1985-07-16 00:00:00

abstract：:We investigated the role of tyrosine phosphorylation of FAK in the stretch-induced MAPKs (extracellular signal-regulated kinase (ERK), p38MAPK) activation in mutant FAK-transfected fibroblasts. In response to uniaxial cyclic stretch (1 Hz, 120% in length), the levels of tyrosine phosphorylation of the Tyr-397 and Tyr-...

journal_title：Biochemical and biophysical research communications

authors： Wang JG,Miyazu M,Matsushita E,Sokabe M,Naruse K

更新日期：2001-10-26 00:00:00

abstract：:Using a specific radioimmunoassay we have isolated and sequenced a new 45-amino acid peptide from rat atria which exhibits similar physiological and pharmacological properties to rat atrial natriuretic peptide (rANP). We have termed the new peptide iso-rANP, because of its functional and structural similarities to rAN...

journal_title：Biochemical and biophysical research communications

authors： Flynn TG,Brar A,Tremblay L,Sarda I,Lyons C,Jennings DB

更新日期：1989-06-15 00:00:00

abstract：:Saposins (SAPs) are small glycoproteins required for activation of sphingolipid hydrolysis by lysosomal enzymes. Four SAPs, SAP-A, -B, -C, and -D, are proteolytically cleaved from a single gene product termed prosaposin. The mature coding sequence of human SAP-A tagged with 6-histidine was expressed in Pichia pastoris...

journal_title：Biochemical and biophysical research communications

authors： Yamada M,Inui K,Hamada D,Nakahira K,Yanagihara K,Sakai N,Nishigaki T,Ozono K,Yanagihara I

更新日期：2004-05-28 00:00:00

abstract：:We investigated the possible involvement of neuromedin U (NMU) and neuromedin S (NMS) in thermoregulation in rats. Intracerebroventricular (icv) injection of NMU or NMS increased the back surface temperature (BS-T) in a dose-dependent manner during both the light and dark periods. Pre-treatment with the β3 blocker SR5...

journal_title：Biochemical and biophysical research communications

authors： Nakahara K,Akagi A,Shimizu S,Tateno S,Qattali AW,Mori K,Miyazato M,Kangawa K,Murakami N

更新日期：2016-02-19 00:00:00

abstract：:An endopeptidase capable of metabolizing a number of neuropeptides and generating [Met5] and [Leu5] enkephalin from enkephalin-containing peptides is secreted by glioma C6 cells. This neutral endopeptidase that is likely to be a thiol protease, has a Mr of 71KDa and is effective only towards oligopeptides. Its specifi...

journal_title：Biochemical and biophysical research communications

authors： Ferro ES,Tambourgi DV,Gobersztejn F,Gomes MD,Sucupira M,Armelin MC,Kipnis TL,Camargo AC

更新日期：1993-02-26 00:00:00

abstract：:8-Hydroxyguanine (8-OH-Gua) residues were formed in DNA of Ehrlich ascites cells exposed to the carcinogen 4-nitroquinoline 1-oxide. Formation of 8-OH-Gua was confirmed by chemical treatment of calf thymus DNA with the proximate metabolite of this carcinogen, 4-hydroxyaminoquinoline 1-oxide, together with seryl-adenos...

journal_title：Biochemical and biophysical research communications

authors： Kohda K,Tada M,Kasai H,Nishimura S,Kawazoe Y

更新日期：1986-09-14 00:00:00

abstract：:The effects of dopamine (DA) on 45Ca2+ ion movement and prolactin release in dispersed female rat anterior pituitary cells were studied to elucidate the mechanism for DA reduction of intracellular calcium levels. In 45Ca2+ prelabeled cells, DA inhibited fractional calcium efflux and prolactin release simultaneously an...

journal_title：Biochemical and biophysical research communications

authors： Login IS,Judd AM,MacLeod RM

更新日期：1988-03-15 00:00:00

abstract：:Prostaglandin E(2) (PGE(2)) has been shown to negatively regulate adipogenesis. To explore to what extent PGE(2) inhibits the differentiation of cells to adipocytes and to examine whether its effect could be due to EP4 receptor signaling, we used microarrays to analyze the gene expression profiles of 3T3-L1 cells expo...

journal_title：Biochemical and biophysical research communications

authors： Sugimoto Y,Tsuboi H,Okuno Y,Tamba S,Tsuchiya S,Tsujimoto G,Ichikawa A

更新日期：2004-09-24 00:00:00

abstract：:ELOVL3 is involved in elongating saturated and monounsaturated fatty acids, and is a critical enzyme for lipid accumulation in brown adipocytes during the early phase of tissue recruitment. In addition, ELOVL3 is related to increased fatty acid oxidation in brown adipocytes. However, the potential functions of ELOVL3 ...

journal_title：Biochemical and biophysical research communications

authors： Liu D,Xu JH,Tong HL,Li SF,Yan YQ

更新日期：2017-12-02 00:00:00

abstract：:Genes for the human vacuolar type H(+)-ATPase proteolipid (16-kDa) subunit were cloned and their nucleotide sequences were determined. Comparison of the deduced sequences indicated that at least four genes including pseudogenes are present in the human genome. One of them corresponded to that for the 16-kDa subunit ex...

journal_title：Biochemical and biophysical research communications

authors： Hasebe M,Hanada H,Moriyama Y,Maeda M,Futai M

更新日期：1992-03-16 00:00:00

abstract：:DNA bending has been shown to play a critical role in conservative site-specific DNA recombination reactions such as lambda integration. V(D)J recombination, the only mammalian site directed recombination system, is directed by recombinational signal sequences composed of heptamer, nonamer and spacer elements. The non...

journal_title：Biochemical and biophysical research communications

authors： Andrews R,Halligan NL,Halligan BD

更新日期：1993-05-28 00:00:00

abstract：:Muscarinic receptor extracted from porcine atria in digitonin-cholate copurified with Galpha(o), Galpha(i1-3), and caveolins. The presence of complexes was confirmed by coimmunoprecipitation of the receptor, alpha-subunits, and caveolins in various combinations. Homooligomers of alpha(i2) were detected on Western blot...

journal_title：Biochemical and biophysical research communications

authors： Ma AW,Pawagi AB,Wells JW

更新日期：2008-09-12 00:00:00

abstract：:The cytochrome P-450 catalyzing 26-hydroxylation of C27-steroids (cytochrome P-450(26] was purified from rabbit liver mitochondria. The specific content of the cytochrome P-450 was 13.6 nmol per mg of protein and the 26-hydroxylase activity towards 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha-triol was 31,300 pmol/nmo...

journal_title：Biochemical and biophysical research communications

authors： Dahlbäck H

更新日期：1988-11-30 00:00:00

abstract：:The presence of type I transglutaminase was determined in the neoplastic human keratinocyte line SqCC/Y1 and in normal human epidermal keratinocytes (NHEK) by an in situ radioimmunoassay which corresponded directly with the measurement of type I transglutaminase enzymatic activity. Dexamethasone induced differentiatio...

journal_title：Biochemical and biophysical research communications

authors： Moore KG,Donadio AC,Sartorelli AC

更新日期：1993-04-30 00:00:00

abstract：:We found that high galectin-1 (Gal-1) mRNA levels were associated with invasive squamous cell carcinoma (SCC) cells that expressed Snail, an epithelial-to-mesenchymal transition (EMT) regulator. Both Gal-1 overexpression and soluble Gal-1 treatment accelerated invasion and collective cell migration, along with activat...

journal_title：Biochemical and biophysical research communications

authors： Rizqiawan A,Tobiume K,Okui G,Yamamoto K,Shigeishi H,Ono S,Shimasue H,Takechi M,Higashikawa K,Kamata N

更新日期：2013-11-29 00:00:00

abstract：:The protein kinase C inhibitor C-I reduced superoxide production by human neutrophils in response to phorbol myristate acetate by greater than 50%. In contrast to its effects in oxidative metabolism, 100 microM C-I caused minimal inhibition (5-18%) of lysozyme release in response to phorbol myristate acetate. Enzyme r...

journal_title：Biochemical and biophysical research communications

authors： Salzer W,Gerard C,McCall C

更新日期：1987-10-29 00:00:00

abstract：:Mycoplasma fermentans has been suspected as one of the causative pathogenic microorganisms of rheumatoid arthritis (RA) however, the pathogenic mechanism is still unclear. We, previously, reported that glycolipid-antigens (GGPL-I and III) are the major antigens of M. fermentans. Monoclonal antibody against the GGPL-II...

journal_title：Biochemical and biophysical research communications

authors： Kawahito Y,Ichinose S,Sano H,Tsubouchi Y,Kohno M,Yoshikawa T,Tokunaga D,Hojo T,Harasawa R,Nakano T,Matsuda K

更新日期：2008-05-02 00:00:00

abstract：:Acute respiratory syncytial virus (RSV) infection causes airway inflammation and exacerbates asthma, but the mechanism of inflammation is poorly understood. The role of the STAT-signaling pathway in RSV infection in epithelial cells was examined in this study. DNA microarray analyses of RSV-infected human alveolar typ...

journal_title：Biochemical and biophysical research communications

authors： Kong X,San Juan H,Kumar M,Behera AK,Mohapatra A,Hellermann GR,Mane S,Lockey RF,Mohapatra SS

更新日期：2003-06-27 00:00:00

abstract：:The binding of four potent antimitotic agents, rhizoxin (RZX), phomopsin A (PMS-A), ansamitocin P-3 (ASMP-3), and vinblastine (VLB), to tubulins from RZX-sensitive and -resistant strains of Aspergillus nidulans, Schizosaccharomyces pombe, and Saccharomyces cerevisiae was investigated. Mycelial extracts to which RZX co...

journal_title：Biochemical and biophysical research communications

authors： Li Y,Kobayashi H,Hashimoto Y,Iwasaki S

更新日期：1992-09-16 00:00:00

abstract：:Although, as their names imply, estrogen receptors [ERs] and estrogen-related receptors [ERRs] are related transcription factors, their evolutionary relationships to each other are not fully understood. To elucidate the origins and evolution of ERs and ERRs, we searched for their orthologs in the recently sequenced ge...

journal_title：Biochemical and biophysical research communications

authors： Baker ME

更新日期：2008-10-31 00:00:00