Abstract:
:The presence of type I transglutaminase was determined in the neoplastic human keratinocyte line SqCC/Y1 and in normal human epidermal keratinocytes (NHEK) by an in situ radioimmunoassay which corresponded directly with the measurement of type I transglutaminase enzymatic activity. Dexamethasone induced differentiation of SqCC/Y1 cells caused a marked increase in transglutaminase immunoreactivity and enzymatic activity over non-steroid treated cells in a concentration-related and a time-related fashion. Retinoic acid suppressed the dexamethasone induced increase in type I transglutaminase immunoreactivity in differentiating SqCC/Y1 cells. The type I transglutaminase radioimmunoassay should be useful in studies focusing on the regulation of transglutaminase activity in normal and neoplastic keratinocytes, and for rapidly screening agents for their effects on squamous cell differentiation.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Moore KG,Donadio AC,Sartorelli ACdoi
10.1006/bbrc.1993.1426subject
Has Abstractpub_date
1993-04-30 00:00:00pages
381-5issue
2eissn
0006-291Xissn
1090-2104pii
S0006-291X(83)71426-9journal_volume
192pub_type
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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更新日期:2013-11-22 00:00:00
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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abstract::Hypoxia-inducible factors are crucial in the regulatory process of oxygen homeostasis of vertebrate cells. Inhibition of prolyl hydroxylation of HIF-alpha subunits by prolyl-hydroxylases (PHD1, PHD2 and PHD3) leads to transcription of a greater number of hypoxia responsive genes. We have investigated the subcellular d...
journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
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更新日期:2006-02-03 00:00:00
abstract::The dodecapeptide MCLV3 is a functional peptide, derived from the CLV3 precursor protein, which is a candidate ligand of the CLV1/CLV2 receptor complex that restricts the stem cell population in the shoot apical meristem (SAM). MCLV3 can induce shoot and root meristem consumption, the typical phenotype of transgenic p...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
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更新日期:2008-12-05 00:00:00
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2004.10.079
更新日期:2004-12-10 00:00:00