Dehydrogenase binding to the 3'-untranslated region of GLUT1 mRNA.

Abstract:

:Employing RNA gel mobility shift assays we detected specific binding events between several dehydrogenases and various regions of the GLUT1 mRNA 3'-untranslated region. In particular, the enzymes glyceraldehyde 3-phosphate dehydrogenase (G3PDH), lactate dehydrogenase (LDH), and glucose 6-phosphate dehydrogenase (G6PDH) bound to the GLUT1 3'-UTR while isocitrate dehydrogenase (IDH) and glutamate dehydrogenase (GluDH) did not. Comparison of shifts obtained with purified dehydrogenases to those obtained using authentic cell extracts indicate that G3PDH and G6PDH may play a role in the intact cell.

authors

McGowan K,Pekala PH

doi

10.1006/bbrc.1996.0541

subject

Has Abstract

pub_date

1996-04-05 00:00:00

pages

42-5

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(96)90541-0

journal_volume

221

pub_type

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