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Prototype foamy virus integrase is promiscuous for target choice.

Abstract：

:Retroviruses have two essential activities: reverse transcription and integration. The viral protein integrase (IN) covalently joins the viral cDNA genome to the host DNA. Prototype foamy virus (PFV) IN has become a model of retroviral intasome structure. However, this retroviral IN has not been well-characterized biochemically. Here we compare PFV IN to previously reported HIV-1 IN activities and discover significant differences. PFV IN is able to utilize the divalent cation calcium during strand transfer while HIV-1 IN is not. HIV-1 IN was shown to completely commit to a target DNA within 1 min, while PFV IN is not fully committed after 60 min. These results suggest that PFV IN is more promiscuous compared to HIV-1 IN in terms of divalent cation and target commitment.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Mackler RM,Lopez MA,Osterhage MJ,Yoder KE

doi

10.1016/j.bbrc.2018.07.031

subject

Has Abstract

pub_date

2018-09-10 00:00:00

pages

1241-1246

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(18)31526-2

journal_volume

503

pub_type

杂志文章

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