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Intracellular delivery of HSP70 using HIV-1 Tat protein transduction domain.

Abstract：

:Heat shock protein 70 (HSP70) is an intracellular stress protein that confers cytoprotection to a variety of cellular stressors. Several lines of evidence have suggested that augmentation of the heat shock response by increasing the expression of HSP70 represents a potential therapeutic strategy for the treatment of critically ill patients. The Tat protein of human immunodeficiency virus 1 (HIV-1) has been used previously to deliver functional cargo proteins intracellularly when added exogenously to cultured cells. We generated a Tat-HSP70 fusion protein using recombinant methods and treated HSF -/- cells with either Tat-HSP70 or recombinant HSP70 prior to exposure to hyperoxia or lethal heat shock. We showed that biologically active, exogenous HSP70 can be delivered into cells using the HIV-1 Tat protein, and that the Tat-mediated delivery of HSP70 confers cytoprotection against thermal stress and hyperoxia and may represent a novel approach to augmenting intracellular HSP70 levels.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Wheeler DS,Dunsmore KE,Wong HR

doi

10.1016/s0006-291x(02)02986-8

subject

Has Abstract

pub_date

2003-01-31 00:00:00

pages

54-9

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006291X02029868

journal_volume

301

pub_type

杂志文章

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