Action of apoptotic endonuclease DNase gamma on naked DNA and chromatin substrates.

abstract：:Homologous-recombination (HR)-dependent repair defective cells are hypersensitive to poly (ADP-ribose) polymerase (PARP) inhibitors. Combinations of defective HR pathway and PARP inhibitors have been an effective chemotherapeutic modality. We previously showed that knockdown of the WD40-repeat containing protein, Uaf1...

journal_title：Biochemical and biophysical research communications

authors： Choi YE,Park E

更新日期：2015-03-13 00:00:00

abstract：:We established a simple and highly efficient method for in vivo gene transfer using HVJ (Sendai virus) and liposomes. Plasmid DNA and high mobility group 1 (HMG1) protein were co-encapsulated in liposomes by agitation and sonication and were co-introduced into cells by HVJ-mediated membrane fusion. pACT SVT DNA, as a ...

journal_title：Biochemical and biophysical research communications

authors： Tomita N,Higaki J,Morishita R,Kato K,Mikami H,Kaneda Y,Ogihara T

更新日期：1992-07-15 00:00:00

abstract：:Pancreatic adenocarcinoma is currently one of the leading causes of cancer-related death worldwide. The high rate of mortality in pancreatic cancer patients is due to the inability to detect early-stage disease and the disease being highly refractory to therapy. Gemcitabine has been the standard chemotherapy for advan...

journal_title：Biochemical and biophysical research communications

authors： Ahn HM,Kim DG,Kim YJ

更新日期：2020-06-25 00:00:00

abstract：:The sum of the magnetic anisotropy and polarization contributions to the magnetic shielding constants of the alpha protons is calculated as a function of the torsion angles about the NC alpha (phi) and C alpha C' (psi) bonds of the dipeptide. The results show that the polarization or electric field effect is several f...

journal_title：Biochemical and biophysical research communications

authors： Gresh N,Giessner-Prettre C

更新日期：1990-09-28 00:00:00

abstract：:S100A8 and S100A9 are members of the S100A8 protein family that exist as homodimers and heterodimers in neutrophils, monocytes, and macrophages. Recent studies have shown the pivotal roles of S100A8 and S100A9 in the propagation of inflammation and keratinocyte proliferation in psoriasis. We found significant up-regul...

journal_title：Biochemical and biophysical research communications

authors： Lee Y,Jang S,Min JK,Lee K,Sohn KC,Lim JS,Im M,Lee HE,Seo YJ,Kim CD,Lee JH

更新日期：2012-07-13 00:00:00

abstract：:Despite their advantages in analysis, 4D NMR experiments are still infrequently used as a routine tool in protein NMR projects due to the long duration of the measurement and limited digital resolution. Recently, new acquisition techniques for speeding up multidimensional NMR experiments, such as nonlinear sampling, i...

journal_title：Biochemical and biophysical research communications

authors： Shigemitsu Y,Ikeya T,Yamamoto A,Tsuchie Y,Mishima M,Smith BO,Güntert P,Ito Y

更新日期：2015-02-06 00:00:00

abstract：:To investigate the maturation of neonatal erythrocytes and kinetics of globin expression, we induced CD34(+) cells purified from human umbilical cord blood to erythroid differentiation in different suspension culture systems containing human cord serum (CHS) and adult serum (AHS), respectively. The zeta- to alpha-glob...

journal_title：Biochemical and biophysical research communications

authors： Zhang X,Luo YM,Ma YN,Zhang JW

更新日期：2005-10-14 00:00:00

abstract：:The physical and catalytic properties of 5 histidine-serine mutants of human 5-lipoxygenase (5-LO) have been characterized. The mutants HS363, HS391 and HS400 have activities, pH optima and stabilities similar to those of the wild type enzyme. The iron content of each of these mutants is 0.30-0.53 mol Fe/mol 5-LO whic...

journal_title：Biochemical and biophysical research communications

authors： Percival MD,Ouellet M

更新日期：1992-08-14 00:00:00

abstract：:MicroRNAs (miRNAs) can negatively regulate gene expression and also induce or inhibit viral replication. In the present study, we found 10 miRNAs were differentially expressed in a stable HBV-producing cell line (HepG2.2.15) compared with its control cell line (HepG2) by miRNA array analysis. miR-501 was significantly...

journal_title：Biochemical and biophysical research communications

authors： Jin J,Tang S,Xia L,Du R,Xie H,Song J,Fan R,Bi Q,Chen Z,Yang G,Liu J,Shi Y,Fan D

更新日期：2013-01-25 00:00:00

abstract：:12-O-tetradecanoyl phorbol 13-acetate (TPA) and 1,2-dioctanoyl-sn-glycerol (diC8) activate protein kinase C (PKC) in transformed fetal bovine aortic endothelial GM 7373 cells. Both molecules cause a similar increase in membrane-associated PKC activity and in the phosphorylation of a PKC-specific endogenous 87-kDa subs...

journal_title：Biochemical and biophysical research communications

authors： Maier JA,Presta M,Ragnotti G

更新日期：1989-04-28 00:00:00

abstract：:Cultured bovine pulmonary artery endothelial cells contain a second peptidyl dipeptidase, distinct from angiotensin-converting enzyme, present in an inactive form associated with a non-dialyzable inhibitor. Partial purification by glycine affinity chromatography separates enzyme from inhibitor to yield a preparation w...

journal_title：Biochemical and biophysical research communications

authors： Lanzillo JJ,Dasarathy Y,Stevens J,Fanburg BL

更新日期：1986-01-29 00:00:00

abstract：:Various tumor cells express interleukin 7 (IL-7) and IL-7 variants. IL-7 has been confirmed to stimulate solid tumor cell proliferation. However, the effect of IL-7 variants on tumor cell proliferation remains unclear. In this study, we evaluated the role of IL-7δ5 (an IL-7 variant lacking exon 5) on proliferation and...

journal_title：Biochemical and biophysical research communications

authors： Pan D,Liu B,Jin X,Zhu J

更新日期：2012-06-15 00:00:00

abstract：:Legionella pneumophila is a flagellated pathogenic bacterium that causes atypical pneumonia called Legionnaires' disease. The flagellum plays a key role in the pathogenesis of L. pneumophila in the host. The protein FlgL forms a junction between the flagellar hook and filament and has been reported to elicit the host ...

journal_title：Biochemical and biophysical research communications

authors： Song WS,Hong HJ,Yoon SI

更新日期：2020-08-20 00:00:00

abstract：:We have investigated the status of the MTS2 gene, encoding the cyclin-dependent kinase (CDK) inhibitor p15, in 32 glioblastomas. Semi-quantitative PCR identified 7 tumors in which the amplified material was 18.6% of controls and 7 in which was 48.0%, suggesting the occurrence of homozygous and hemizygous deletions, re...

journal_title：Biochemical and biophysical research communications

authors： Tenan M,Benedetti S,Finocchiaro G

更新日期：1995-12-05 00:00:00

abstract：:Although caffeine alters sleep in many animals, whether or not it affects mammalian circadian clocks remains unknown. Here, we found that incubating cultured mammalian cell lines, human osteosarcoma U2OS cells and mouse fibroblast NIH3T3 cells, with caffeine lengthened the period of circadian rhythms. Adding caffeine ...

journal_title：Biochemical and biophysical research communications

authors： Oike H,Kobori M,Suzuki T,Ishida N

更新日期：2011-07-08 00:00:00

abstract：:In general, viral infection is supposed to induce stress responses in the host cell. However, very few detailed observations about virus-induced stress responses have been reported. Here we investigated specific stress responses in Vero cells infected with Sindbis virus (SV), a single-stranded RNA virus, acute infecti...

journal_title：Biochemical and biophysical research communications

authors： Nakatsue T,Katoh I,Nakamura S,Takahashi Y,Ikawa Y,Yoshinaka Y

更新日期：1998-12-09 00:00:00

abstract：:The aim of this work was to study the time course of the oxidative metabolism in mice lung after exposure to ambient particles (ROFA). Swiss mice were intranasally instilled with a ROFA suspension (0.20 mg/kg). Animals were sacrificed 1 or 3 h after the exposure. Eighty percentage of increased oxygen consumption was o...

journal_title：Biochemical and biophysical research communications

authors： Magnani ND,Marchini T,Tasat DR,Alvarez S,Evelson PA

更新日期：2011-09-09 00:00:00

abstract：:N-terminal of Leu-norleucinal or Leu-methioninal was modified to obtain a cell penetrative peptide inhibitor against calpain. Benzyloxycarbonyl (Z) derivatives had less active against papain than phenylbutyryl derivatives and leupeptin. Z-Leu-nLeu-H (calpeptin) was more sensitive to calpain I than Z-Leu-Met-H and leup...

journal_title：Biochemical and biophysical research communications

authors： Tsujinaka T,Kajiwara Y,Kambayashi J,Sakon M,Higuchi N,Tanaka T,Mori T

更新日期：1988-06-30 00:00:00

abstract：:Dexamethasone (Dex) induces direct cytotoxicity to cultured osteoblasts. The benzimidazole derivative compound 991 ("C991") is a novel and highly-efficient AMP-activated protein kinase (AMPK) activator. Here, in both MC3T3-E1 osteoblastic cells and primary murine osteoblasts, treatment with C991 activated AMPK signali...

journal_title：Biochemical and biophysical research communications

authors： Xu YY,Chen FL,Ji F,Fei HD,Xie Y,Wang SG

更新日期：2018-01-01 00:00:00

abstract：:Cellular Ca(2+) homeostasis is maintained through the integrated and coordinated function of Ca(2+) transport molecules, Ca(2+) buffers and sensors. These molecules are associated with the plasma membrane and different cellular compartments, such as the cytoplasm, nucleus, mitochondria, and cellular reticular network,...

journal_title：Biochemical and biophysical research communications

authors： Krebs J,Agellon LB,Michalak M

更新日期：2015-04-24 00:00:00

abstract：:Airway viral infection disturbs the health-related quality of life. B7-H1 (also known as PD-L1) is a coinhibitory molecule associated with the escape of viruses from the mucosal immunity, leading to persistent infection. Most respiratory viruses generate double-stranded (ds) RNA during replication. The stimulation of ...

journal_title：Biochemical and biophysical research communications

authors： Kan-o K,Matsumoto K,Asai-Tajiri Y,Fukuyama S,Hamano S,Seki N,Nakanishi Y,Inoue H

更新日期：2013-05-31 00:00:00

abstract：:Cell cycle arrest is essential for initiation of muscle differentiation in myoblasts. Given the previously described essential role for p38 MAPK in myogenesis, we undertook the present study to investigate the role of p38 MAPK in the cell cycle arrest that initiates muscle differentiation. p38 MAPK activity increased ...

journal_title：Biochemical and biophysical research communications

authors： Lee J,Hong F,Kwon S,Kim SS,Kim DO,Kang HS,Lee SJ,Ha J,Kim SS

更新日期：2002-11-15 00:00:00

abstract：:Cartilage-derived growth factors, enhance proteoglycan synthesis in cultured chick-embryo chondrocytes, and have almost no effect on cell proliferation. Addition of cartilage derived growth factors to cartilage cells loaded with the fluorescent Ca2+ indicator quin 2, caused a rapid, concentration dependent decrease in...

journal_title：Biochemical and biophysical research communications

authors： Eilam Y,Beit-Or A,Nevo Z

更新日期：1985-10-30 00:00:00

abstract：:The binding site(s) in rabbit liver fructose-1,6-bisphosphatase for the active site binding ligand, fructose 6-phosphate, and the inhibitor, fructose 2,6-bisphosphate, have been investigated by using nuclear magnetic resonance spectroscopy. The distance from a nitroxide spin label to the bound ligands and the distance...

journal_title：Biochemical and biophysical research communications

authors： Liu F,Roy M,Fromm HJ

更新日期：1989-06-15 00:00:00

abstract：:18,54-SF cells, which secrete rat insulin-like growth factor II (rIGF-II), have abundant type 2 IGF receptors. We have purified the type 2 receptor from these cells by solubilization of crude membranes in Triton X-100, followed by chromatography on agarose-immobilized rIGF-II. A partially purified receptor preparation...

journal_title：Biochemical and biophysical research communications

authors： Rosenfeld RG,Hodges D,Pham H,Lee PD,Powell DR

更新日期：1986-07-16 00:00:00

abstract：:Apobec1 edits the ApoB mRNA by deaminating nucleotide C(6666), which results in a codon change from Glutamate to stop, and subsequent expression of a truncated protein. Apobec1 is regulated by ACF (Apobec1 complementation factor) and hnRNPQ, which contains an N-terminal "acidic domain" (AcD) of unknown function, three...

journal_title：Biochemical and biophysical research communications

authors： Quaresma AJ,Oyama S Jr,Barbosa JA,Kobarg J

更新日期：2006-11-17 00:00:00

abstract：:Sialoglycans on the cell surface of human colon cancer (HCC) cells have been implicated in cellular adhesion and metastasis. To clarify the role of N-acetylneuraminic acid (NeuAc) linked alpha2,3 to galactose (Gal) on the surface of HCC cells, we studied the intercellular adhesion of HCC cell lines expressing increasi...

journal_title：Biochemical and biophysical research communications

authors： Dimitroff CJ,Pera P,Dall'Olio F,Matta KL,Chandrasekaran EV,Lau JT,Bernacki RJ

更新日期：1999-03-24 00:00:00

abstract：:Hexokinase 1 (HK1) purified from rat brain exhibits protein kinase activity, including autophosphorylation and phosphorylation of histone H2A. This protein kinase activity is observed only in the absence of the HK1 carbohydrate substrate, glucose. Analysis of the ATP-binding domains of the mammalian HK1 protein sequen...

journal_title：Biochemical and biophysical research communications

authors： Adams V,Griffin LD,Gelb BD,McCabe ER

更新日期：1991-06-28 00:00:00

abstract：:It has been emphasized that epithelial injury is closely correlated with airway hyperresponsiveness, which is one of the important pathophysiological characteristics of bronchial asthma. Growth of epithelial cells is important in the mucosal repair processes and is believed to be regulated by growth factors produced b...

journal_title：Biochemical and biophysical research communications

authors： Kobayashi M,Niitsuma T,Hayashi T,Tanaka M,Takizawa H

更新日期：1998-03-06 00:00:00

abstract：:Sirt1 appear to be NAD(+)-dependent deacetylase that deacetylates histones and several non-histone proteins. In this study, we identified Sirt1 as a physical interaction partner of Tip60, which is a mammalian MYST-type histone acetyl-transferase that specifically acetylates histones H2A and H4. Although Tip60 also ace...

journal_title：Biochemical and biophysical research communications

authors： Yamagata K,Kitabayashi I

更新日期：2009-12-25 00:00:00