Many commonly used siRNAs risk off-target activity.

abstract：:The second leader exon and the relative promoter of the mouse liver/bone/kidney-type alkaline phosphatase gene were identified and characterized. The transcription initiation site was determined by S1 mapping analysis. The differential expression of the two alternatively spliced transcripts was assessed in cell lines ...

journal_title：Biochemical and biophysical research communications

authors： Studer M,Terao M,Gianni M,Garattini E

更新日期：1991-09-30 00:00:00

abstract：:The genetic encoding of synthetic or "non-natural" amino acids promises to diversify the functions and structures of proteins. We applied rapid codon-reassignment for creating Escherichia coli strains unable to terminate translation at the UAG "stop" triplet, but efficiently decoding it as various tyrosine and lysine ...

journal_title：Biochemical and biophysical research communications

authors： Mukai T,Yanagisawa T,Ohtake K,Wakamori M,Adachi J,Hino N,Sato A,Kobayashi T,Hayashi A,Shirouzu M,Umehara T,Yokoyama S,Sakamoto K

更新日期：2011-08-12 00:00:00

abstract：:Peroxisome proliferator-activated receptor gamma (PPARγ) participates in the process of insulin resistance (IR), a crucial pathophysiology in non-alcoholic fatty liver disease (NAFLD). Meanwhile, suppressor of cytokine signaling3 (SOCS3) also regulates IR in NAFLD. Both PPARγ and SOCS3 play a role in NAFLD through reg...

journal_title：Biochemical and biophysical research communications

authors： Bi J,Sun K,Wu H,Chen X,Tang H,Mao J

更新日期：2018-04-15 00:00:00

abstract：:The forkhead transcription factor FoxA1 is thought to be involved in mammary tumorigenesis. However, the precise role of FoxA1 in breast cancer development is controversial. We examined expression of FoxA1 in 35 human breast cancer cell lines and compared it with that of ErbB2, a marker of poor prognosis in breast can...

journal_title：Biochemical and biophysical research communications

authors： Yamaguchi N,Ito E,Azuma S,Honma R,Yanagisawa Y,Nishikawa A,Kawamura M,Imai J,Tatsuta K,Inoue J,Semba K,Watanabe S

更新日期：2008-01-25 00:00:00

abstract：:Livers from normal fed or fasted (24h) rats were perfused in vitro to determine whether fatty acid affects the biosynthesis of very low density lipoprotein (VLDL) apoprotein. Oleate stimulated VLDL triacylglycerol output and increased incorporation of L-[4,5-3H]leucine into VLDL apoprotein in both the fed and fasted g...

journal_title：Biochemical and biophysical research communications

authors： Salam WH,Wilcox HG,Heimberg M

更新日期：1985-10-15 00:00:00

abstract：:Here we describe differences in the formation of the epinephrine-induced Ca2+ transients between parent and Ki-ras-transformed NIH3T3 fibroblasts. These transients proved to be the results of an efflux from both the thapsigargin-sensitive and -insensitive intracellular pools. While the epinephrine-induced detachment o...

journal_title：Biochemical and biophysical research communications

authors： Tóth S,Fu T,Nozawa Y,Csaba G

更新日期：1993-01-29 00:00:00

abstract：:CD40-stimulated human B lymphocytes are highly permissive to a productive infection by the human immunodeficiency virus type 1. In these cells, nuclear factors involved in activation of the HIV-1 LTR, which contains the transcriptional control elements of the virus, are unknown. Transient expression assays with plasmi...

journal_title：Biochemical and biophysical research communications

authors： Lapointe R,Lemieux R,Darveau A

更新日期：1996-12-24 00:00:00

abstract：BACKGROUND:PTK6 is involved in cell proliferation, invasion and migration. Patients with lower PTK6 expression predicts poor prognosis of LSCC. However, the mechanism of PTK6 in LSCC progression remains unclear. We investigated the role of PTK6 in the pathogenesis of LSCC. METHODS:Human LSCC tissues and paired adjacen...

journal_title：Biochemical and biophysical research communications

authors： Wu D,Zhang X,Liu Z,Yan H,Mai J,Zhao Z,Zhong Q,Liu X

更新日期：2018-09-10 00:00:00

abstract：:gamma S-Crystallin from shark eye lenses, formerly termed beta s crystallin in mammalian lenses, is structurally characterized in this study by cDNA cloning and sequencing. To facilitate sequence characterization of gamma S-crystallin possessing intermediate structural properties between beta- and gamma-crystallins, c...

journal_title：Biochemical and biophysical research communications

authors： Pan FM,Chuang MH,Chiou SH

更新日期：1997-11-07 00:00:00

abstract：:The chaperonin from the acidothermophilic archaeon, Sulfolobus sp. Strain 7, is composed of two kinds of subunits designated as Scp alpha and Scp beta. In this study, we characterized the recombinant Scp alpha and Scp beta, which were separately expressed in Escherichia coli. Both of them were able to assemble to homo...

journal_title：Biochemical and biophysical research communications

authors： Yoshida T,Yohda M,Suzuki M,Yazaki K,Miura K,Endo I

更新日期：1998-01-26 00:00:00

abstract：:T-type Ca(2+) channels have been implicated in tremorogenesis and motor coordination. The α1 subunit of the Ca(V)3.1 T-type Ca(2+) channel is highly expressed in motor pathways in the brain, but knockout of the Ca(V)3.1 gene (α(1G)(-/-)) per se causes no motor defects in mice. Thus, the role of Ca(V)3.1 channels in mo...

journal_title：Biochemical and biophysical research communications

authors： Chang KY,Park YG,Park HY,Homanics GE,Kim J,Kim D

更新日期：2011-06-24 00:00:00

abstract：:Human thyroperoxidase (TPO) ectodomain is successively made of myeloperoxidase-, complement control protein repeat-, and epidermal growth factor-like gene modules. However, the TPO immunodominant region targeted by autoantibodies from patients with an autoimmune thyroid disease has not been mapped on the molecule. Her...

journal_title：Biochemical and biophysical research communications

authors： Blanchin S,Estienne V,Guo J,Rapoport B,McLachlan SM,Carayon P,Ruf J

更新日期：2002-08-02 00:00:00

abstract：:The effects of different lysophospholipids on endothelium-dependent hyperpolarization by acetylcholine were examined in rat mesenteric arteries. Lysophosphatidylcholine with 14 or longer carbon acyl chain significantly inhibited the hyperpolarization, while that with 12 or lesser carbon acyl chain was without effect. ...

journal_title：Biochemical and biophysical research communications

authors： Fukao M,Hattori Y,Kanno M,Sakuma I,Kitabatake A

更新日期：1996-10-14 00:00:00

abstract：:We have used human neuroblastoma NB-OK1 cells to investigate the regulation of neurite outgrowth. Carbachol suppressed forskolin-stimulated neurite outgrowth in NB-OK1 cells although forskolin-stimulated cAMP levels were enhanced. The dose-response curve for this suppression was very similar to that for stimulation of...

journal_title：Biochemical and biophysical research communications

authors： Nakagawa-Yagi Y,Saito Y,Takada Y,Nakamura H

更新日期：1992-01-15 00:00:00

abstract：:Fission yeast cell division is initiated by the cdc2/cdc13-cyclin protein kinase which in its catalytically active state comprises the mitotic inducer. During interphase the cdc2/cyclin complex is assembled in an inactive state that requires cdc25+ gene function for M-phase activation. The cdc25+ product, a 76 kd phos...

journal_title：Biochemical and biophysical research communications

authors： Ducommun B,Draetta G,Young P,Beach D

更新日期：1990-02-28 00:00:00

abstract：BACKGROUND:Quercetin is a plant polyphenol from the flavonoid group that plays a fundamental role in controlling homeostasis due to its potent antioxidant properties. However, quercetin has extremely low water solubility, which is a major challenge in drug absorption. METHOD:In this study, we described a simple method...

journal_title：Biochemical and biophysical research communications

authors： Aghapour F,Moghadamnia AA,Nicolini A,Kani SNM,Barari L,Morakabati P,Rezazadeh L,Kazemi S

更新日期：2018-06-12 00:00:00

abstract：:Mycobacterium tuberculosis (TB) small heat shock protein Hsp16.3 was found to be a major membrane protein that is most predominantly expressed under oxidative stress and is localized to the thickened cell envelope. Gene knock-out studies indicate that the Hsp16.3 protein is required for TB to grow in its host macropha...

journal_title：Biochemical and biophysical research communications

authors： Abulimiti A,Qiu X,Chen J,Liu Y,Chang Z

更新日期：2003-05-23 00:00:00

abstract：:Autoimmune diseases are characterized by the presence of autoantibodies often restricted to host proteins exhibiting charge rich domains. Charged polypeptides elicit strong immune responses, and cationized bovine serum albumin and other cationic proteins are significantly more immunogenic than their less charged count...

journal_title：Biochemical and biophysical research communications

authors： Dohlman JG,Pillion DJ,Rokeach LA,Ramprasad MP

更新日期：1991-12-16 00:00:00

abstract：:We have discovered two new exons in the mouse fibroblast growth factor 2 (FGF-2 or bFGF) gene that can be alternatively spliced to the second coding exon of the gene. The newly identified exons 1b and 1c are located at, respectively, approximately 19 and 32 kb downstream of the canonical exon 1a. Using RT-PCR analysis...

journal_title：Biochemical and biophysical research communications

authors： Dirks RP,Potter SJ,Griep AE

更新日期：2001-10-05 00:00:00

abstract：:An affinity matrix for the purification of norsolorinic acid dehydrogenase, an enzyme involved in aflatoxin biosynthesis, was prepared by coupling norsolorinic acid to an agarose gel. This matrix was found to be ineffective in isolating active enzyme, and was therefore modified by methylation, using diazomethane. The ...

journal_title：Biochemical and biophysical research communications

authors： Chuturgoon AA,Dutton MF,Berry RK

更新日期：1990-01-15 00:00:00

abstract：:Vasoactive intestinal contractor (VIC) is a member of the endothelin (ET) family. We have investigated the regional distribution of VIC/ET-2 and of ET-1 gene expression in the adult murine brain and pituitary gland. We used real-time quantitative reverse transcription-linked polymerase chain reaction. VIC/ET-2 gene ex...

journal_title：Biochemical and biophysical research communications

authors： Masuo Y,Ishikawa Y,Kozakai T,Uchide T,Komatsu Y,Saida K

更新日期：2003-01-17 00:00:00

abstract：:It has been shown that anti-cancer drug induces secretion of serotonin (5-HT) from small intestine which activates serotonin type 3 (5-HT(3)) receptor to cause nausea and vomiting. In general, antagonist for 5-HT(3) receptor is used as anti-emetics during chemotherapy. However, we found that anti-cancer drug irinoteca...

journal_title：Biochemical and biophysical research communications

authors： Nakamura Y,Ishida Y,Yamada T,Shimada S

更新日期：2011-11-18 00:00:00

abstract：:The death of nigral neurons in Parkinson's disease is thought to involve the formation of the endogenous neurotoxin, 5-S-cysteinyl-dopamine. In the present study, we show that the polyphenols, (+)-catechin and caffeic acid, which contain a catechol moiety, inhibit tyrosinase-induced formation of 5-S-cysteinyl-dopamine...

journal_title：Biochemical and biophysical research communications

authors： Vauzour D,Vafeiadou K,Spencer JP

更新日期：2007-10-19 00:00:00

abstract：:The thrombopoietin receptor, also known as c-Mpl, is a member of the cytokine superfamily, which regulates the differentiation of megakaryocytes and formation of platelets by binding to its ligand, thrombopoietin (TPO), through Janus kinase (JAK)-signal transducer and activator of transcription (STAT) signaling. The l...

journal_title：Biochemical and biophysical research communications

authors： Sasazawa Y,Sato N,Suzuki T,Dohmae N,Simizu S

更新日期：2015-12-04 00:00:00

abstract：:Lysinuric protein intolerance (LPI; MIM 222700) is an autosomal recessive disorder characterized by defective transport of cationic amino acids lysine, arginine and ornithine. The defect is localized in the basolateral membrane of polar epithelial cells of the renal tubules and intestine. The SLC7A7 (solute carrier fa...

journal_title：Biochemical and biophysical research communications

authors： Toivonen M,Mykkänen J,Aula P,Simell O,Savontaus ML,Huoponen K

更新日期：2002-03-15 00:00:00

abstract：:Recently we have identified mRNA encoding a hitherto unknown mammalian X,K-ATPase beta-subunit expressed predominantly in muscle tissue (Pestov, N. B. et al. (1999) FEBS Lett. 456, 243-248). Here we demonstrate the existence of the predicted protein, designated as beta(m) (beta(muscle)), in human adult skeletal muscle...

journal_title：Biochemical and biophysical research communications

authors： Pestov NB,Korneenko TV,Zhao H,Adams G,Shakhparonov MI,Modyanov NN

更新日期：2000-10-22 00:00:00

abstract：:We have obtained the optimized geometrical structure of 2-(1-carboxy-1-hydroxyethyl)-3,4-dimethylthiazolium dipolar ion and investigated its geometric and electric changes during decarboxylation process by the MNDO-PM3 method, a molecular orbital method. The salient features of the optimized structure are that the dih...

journal_title：Biochemical and biophysical research communications

authors： Nakajima Y,Sakagishi Y,Shiibashi M,Suzuki Y,Kato H

更新日期：1993-08-31 00:00:00

abstract：:Ribosome modulation factor (RMF) is involved in stabilization of ribosomes during the transition from exponential growth to the stationary growth phase in Escherichia coli. A deficiency of RMF is known to reduce cell viability. Overaccumulation of spermidine also leads to a decrease in cell viability and to a decrease...

journal_title：Biochemical and biophysical research communications

authors： Apirakaramwong A,Fukuchi J,Kashiwagi K,Kakinuma Y,Ito E,Ishihama A,Igarashi K

更新日期：1998-10-20 00:00:00

abstract：:Little is known about determinants regulating expression of Mannan-binding lectin associated serine protease-2 (MASP-2), the effector component of the lectin pathway of complement activation. Comparative bioinformatic analysis of the MASP2 promoter regions in human, mouse, and rat, revealed conservation of two putativ...

journal_title：Biochemical and biophysical research communications

authors： Unterberger C,Hanson S,Klingenhoff A,Oesterle D,Frankenberger M,Endo Y,Matsushita M,Fujita T,Schwaeble W,Weiss EH,Ziegler-Heitbrock L,Stover C

更新日期：2007-12-28 00:00:00

abstract：:Of two major isoforms of angiotensin II receptors, AT1 and AT2, biological roles of AT2 remain unclear. Using vascular smooth muscle cells, we investigated the regulation of expression of AT2 by growth factors in comparison with that of AT1. The cultured rat aorta smooth muscle cells had detectable AT2 binding sites, ...

journal_title：Biochemical and biophysical research communications

authors： Kambayashi Y,Bardhan S,Inagami T

更新日期：1993-07-15 00:00:00