Abstract:
:The second leader exon and the relative promoter of the mouse liver/bone/kidney-type alkaline phosphatase gene were identified and characterized. The transcription initiation site was determined by S1 mapping analysis. The differential expression of the two alternatively spliced transcripts was assessed in cell lines of different origin and in various tissues by polymerase chain reaction and RNase mapping analysis. The first promoter is active in embryo derived cells, whereas the second promoter is silent in basal conditions but it is activated by dibutyryl cAMP in fibroblastic cells. In the whole animal, the transcript driven by the first promoter is found in most tissues albeit at different levels, while the one driven by the second promoter is specifically expressed at high levels only in the heart.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Studer M,Terao M,Gianni M,Garattini Edoi
10.1016/0006-291x(91)91722-osubject
Has Abstractpub_date
1991-09-30 00:00:00pages
1352-60issue
3eissn
0006-291Xissn
1090-2104pii
0006-291X(91)91722-Ojournal_volume
179pub_type
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