Abstract:
:The high affinity laminin receptor is differentially expressed in metastasis. We now report that there are multiple copies (6 +/- 1) of the laminin receptor gene in the murine genome of normal diploid cells as well as in cell lines derived from cancer cells. We have analyzed three distinct cDNA clones isolated from an Okayama-Berg cDNA library of transformed mouse fibroblasts that may represent transcripts of three different laminin receptor genes. Polymorphic changes include insertion of bases at the 5' terminus, a base substitution within the coding region resulting in an amino acid change from phenylalanine to leucine, a base substitution obliterating a polyadenylation signal, as well as changes in the length of the 3' untranslated domains. The discovery of multiple transcripts of laminin receptor genes suggests that there is a strong selective pressure to maintain laminin receptor expression in murine cells.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Fernández MT,Castronovo V,Rao CN,Sobel MEdoi
10.1016/s0006-291x(05)81203-3subject
Has Abstractpub_date
1991-02-28 00:00:00pages
84-90issue
1eissn
0006-291Xissn
1090-2104pii
S0006-291X(05)81203-3journal_volume
175pub_type
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journal_title:Biochemical and biophysical research communications
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