Abstract:
:The Mrr protein of Escherichia coli K12 is a cryptic Type IV restriction endonuclease whose activity appears to be triggered by high pressure stress. In this report we used high pressure to isolate and analyze several Mrr mutants, and generated a new structural model of the Mrr protein. The activity of a number of spontaneous and strategically constructed Mrr mutants is discussed in the light of this model, providing a first insight into the structure-function relationships of the Mrr enzyme.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Orlowski J,Mebrhatu MT,Michiels CW,Bujnicki JM,Aertsen Adoi
10.1016/j.bbrc.2008.10.064subject
Has Abstractpub_date
2008-12-19 00:00:00pages
862-6issue
3eissn
0006-291Xissn
1090-2104pii
S0006-291X(08)02036-6journal_volume
377pub_type
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