Abstract:
:Trichosanthin (TCS) is a type I ribosome-inactivating protein that inhibits the replication of both human immunodeficiency virus type 1 (HIV-1) and herpes simplex virus type 1 (HSV-1). The mechanism of inhibition is not clear. This investigation explored the effects of TCS on the stages of HSV-1 infection in Hep-2 cells, from attachment to release. We demonstrated that TCS reduced HSV-1 antigen and DNA content and interfered with viral replication as early as 3-15 h after infection. TCS had no effect on HSV-1 attachment, penetration or immediate-early gene expression. However, the expression of early and late genes and virion release were diminished. In summary, this study demonstrates that TCS primarily affects HSV-1 replication in Hep-2 cells during the early to late infection period.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
He DX,Tam SCdoi
10.1016/j.bbrc.2010.10.080subject
Has Abstractpub_date
2010-11-26 00:00:00pages
670-5issue
4eissn
0006-291Xissn
1090-2104pii
S0006-291X(10)01958-3journal_volume
402pub_type
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