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Switches in 6-phosphofructo-2-kinase isoenzyme expression during rat sperm maturation.

Abstract：

:Here we analyzed Pfkfb3 and Pfkfb4 gene expression in rat testis development, isolated testicular cells and spermatozoa. Real time RT-PCR analysis during testis development showed the maximum expression of Pfkfb3 in pre-puber samples and of Pfkfb4 in adult samples. Western blot analysis showed that uPFK-2 protein, a product of Pfkfb3 gene, was present in all the cell types forming the seminiferous epithelium (Sertoli, interstitial and spermatogenic cells). In contrast, tPFK-2, a product of Pfkfb4 gene, was restricted to spermatogenic cells. Confocal analyses by indirect immunofluorescence also corroborated this expression pattern. Immunoblotting studies of isolated spermatozoa demonstrated the presence of uPFK-2 only in immature sperm and once spermatozoa became fully functional this isozyme was replaced by the testicular isozyme tPFK-2. Moreover, immunostaining confirmed that tPFK-2 was localized mainly in the acrosomal region of the sperm head and in the mid-piece of the flagellum, where other spermatogenic cell-specific glycolytic enzymes have been found.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Gómez M,Navarro-Sabaté A,Manzano A,Duran J,Obach M,Bartrons R

doi

10.1016/j.bbrc.2009.07.021

subject

Has Abstract

pub_date

2009-09-18 00:00:00

pages

330-5

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(09)01353-9

journal_volume

387

pub_type

杂志文章

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