Abstract:
:Two isoforms of the src-family tyrosine kinase p59fyn have been shown to arise through alternative splicing of exon 7 of the fyn gene. These isoforms have been designated fynT, expressed in hematopoietic cells, and fynB, expressed in the brain. Here, we describe a novel isoform, fynDelta7, in which exon 7 is absent. FynDelta7 mRNA transcripts have been identified in peripheral blood mononuclear cells from all individuals tested thus far and semi-quantitative RT-PCR indicates that this fyn transcript is expressed at near wild-type levels. Transcripts coding for the fynDelta7 isoform can be detected in purified B cells, T cells, NK cells, and monocytes, indicating that it is not lineage specific. We further show that the message isolated is functional using an in vitro expression system and by its expression in COS cells.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Goldsmith JF,Hall CG,Atkinson TPdoi
10.1016/s0006-291x(02)02510-xsubject
Has Abstractpub_date
2002-11-08 00:00:00pages
501-4issue
4eissn
0006-291Xissn
1090-2104pii
S0006291X0202510Xjournal_volume
298pub_type
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