当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Combination of metformin and RG7388 enhances inhibition of growth and induction of apoptosis of ovarian cancer cells through the PI3K/AKT/mTOR pathway.

Combination of metformin and RG7388 enhances inhibition of growth and induction of apoptosis of ovarian cancer cells through the PI3K/AKT/mTOR pathway.

Abstract：

:Ovarian cancer is a gynecological cancer that has the highest mortality rate and is often resistant to conventional treatments. Therefore, development of new therapies is essential. Metformin (MET), which is the priority drug for treatment of type 2 diabetes, has received increasing attention because of its anti-tumor effects. Here, we examined combined anti-tumor effects of MET and RG7388, the only MDM2 (mouse double minute 2 homolog) antagonist that has entered phase III clinical trials, on ovarian cancer cell lines. We examined effects on proliferation by Cell Counting Kit-8 (CCK-8) and colony formation assays, and effects on apoptosis by flow cytometric analysis and Hoechst staining. Western blotting was used to measure protein expression in cells and tissues treated with MET and/or RG7388. Flow cytometry was used to measure reactive oxygen species (ROS). We also examined the effects of MET and/or RG7388 on inhibition of A2780 cell growth in vivo. The combination of MET and RG7388 significantly increased growth inhibition, apoptosis, and ROS of A2780 and SKOV3 cells compared with either agent alone. Additionally, in vitro and in vivo results showed that MET and/or RG7388 inhibited the phosphoinositide 3-kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) pathway and their combination had a stronger effect. Our findings suggest that the combination of MET and RG7388 enhances growth inhibition and apoptosis induction of ovarian cancer cells through the PI3K/AKT/mTOR pathway and accumulation of intracellular ROS.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Cui Y,Zhou J,Rong F

doi

10.1016/j.bbrc.2020.09.135

subject

Has Abstract

pub_date

2020-12-17 00:00:00

pages

665-671

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(20)31895-7

journal_volume

533

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Domains I and II in the 5' nontranslated region of the HCV genome are required for RNA replication.

    abstract：:Hepatitis C virus (HCV), a hepacivirus member of the Flaviviridae family, has a positive-stranded RNA genome, which consists of a single open reading frame (ORF) and nontranslated regions (NTRs) at the 5' and 3' ends. The 5'NTR was found to contain an internal ribosomal entry site (IRES), which is required for the tra...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.6167

    authors： Kim YK,Kim CS,Lee SH,Jang SK

    更新日期：2002-01-11 00:00:00

  • Inhibition of B7-H3 reverses oxaliplatin resistance in human colorectal cancer cells.

    abstract：:B7-H3, an immunoregulatory protein, has been found highly expressed in several cancer types, and involved in cancer cell migration and invasion. Here, we investigated the role of B7-H3 in oxaliplatin resistance in colorectal cancer (CRC) cells. Transient silencing of B7-H3 enhanced oxaliplatin sensitivity by increasin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.07.001

    authors： Zhang P,Chen Z,Ning K,Jin J,Han X

    更新日期：2017-08-26 00:00:00

  • The role of reactive oxygen compounds derived from 6-hydroxydopamine for bone marrow purging from neuroblastoma cells.

    abstract：:6-Hydroxydopamine(6-OHDA), a specific neurotoxin against sympathetic nerve cells, is a drug already used for purging of bone marrow from neuroblastoma cells before autologous bone marrow transplantation. However, we could not detect significant differences in the toxicity of 6-OHDA against neuroblastoma and other tumo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)90397-3

    authors： Bruchelt G,Buck J,Girgert R,Treuner J,Niethammer D

    更新日期：1985-07-16 00:00:00

  • Auxin-regulated changes in protein phosphorylation in pea epicotyls.

    abstract：:Auxins regulate various aspects of plant growth and development. However, the mechanism by which these hormones elicit diverse physiological processes is not clear. We present evidence for the role of auxin in protein phosphorylation and the possible involvement of calmodulin in auxin-induced changes. In the presence ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(87)80055-4

    authors： Reddy AS,Chengappa S,Poovaiah BW

    更新日期：1987-04-29 00:00:00

  • Rab3A delayed catecholamine secretion from bovine adrenal chromaffin cells.

    abstract：:To study the role of Rab3A in regulated secretion, recombinant Rab3A protein plus various guanine nucleotides were perfused into bovine adrenal chromaffin cells via a patch pipette, and depolarization-evoked catecholamine secretion from individual cells was measured by amperometry. Rab3A plus GTP, GDP, and GTP gamma S...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0655

    authors： Lin CG,Pan CY,Kao LS

    更新日期：1996-04-25 00:00:00

  • Rapamycin targeting mTOR and hedgehog signaling pathways blocks human rhabdomyosarcoma growth in xenograft murine model.

    abstract：:Rhabdomyosarcomas (RMS) represent the most common childhood soft-tissue sarcoma. Over the past few decades outcomes for low and intermediate risk RMS patients have slowly improved while patients with metastatic or relapsed RMS still face a grim prognosis. New chemotherapeutic agents or combinations of chemotherapies h...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.05.001

    authors： Kaylani SZ,Xu J,Srivastava RK,Kopelovich L,Pressey JG,Athar M

    更新日期：2013-06-14 00:00:00

  • Differential deficiency of mevalonate kinase and phosphomevalonate kinase in patients with distinct defects in peroxisome biogenesis: evidence for a major role of peroxisomes in cholesterol biosynthesis.

    abstract：:Peroxisomes catalyze a number of essential metabolic functions especially related to lipid metabolism. There is increasing evidence suggesting that peroxisomes are also involved in the synthesis of isoprenoids via the mevalonate pathway at least in rat liver. In order to obtain independent evidence for a role of perox...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8836

    authors： Wanders RJ,Romeijn GJ

    更新日期：1998-06-29 00:00:00

  • Gln3-Gcn4 hybrid transcriptional activator determines catabolic and biosynthetic gene expression in the yeast Saccharomyces cerevisiae.

    abstract：:The yeast Saccharomyces cerevisiae is able to sense the availability and quality of nitrogen sources and the intrinsic variation of amino acid disponibility for protein synthesis. When this yeast is provided with secondary nitrogen sources, transcription of genes encoding enzymes involved in their catabolism is elicit...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.12.075

    authors： Hernández H,Aranda C,Riego L,González A

    更新日期：2011-01-21 00:00:00

  • PprI: a general switch responsible for extreme radioresistance of Deinococcus radiodurans.

    abstract：:Deinococcus radiodurans exhibits an extraordinary ability to withstand the lethal and mutagenic effects of DNA damaging agents, particularly, ionizing radiation. Available evidence indicates that efficient repair of DNA damage and protection of the chromosomal structure are mainly responsible for the radioresistance. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)00965-3

    authors： Hua Y,Narumi I,Gao G,Tian B,Satoh K,Kitayama S,Shen B

    更新日期：2003-06-27 00:00:00

  • Hydration of sodium hyaluronate, NMR study of an oriented sample.

    abstract：:A sample of highly oriented Na-hyaluronate was prepared with a wet--spinning method and equilibrated to 75% relative humidity. Its proton NMR spectrum was recorded as a function of the angle theta between the hyaluronate helix axis and the external magnetic field. It appeared as a singlet with an angle-dependent line ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(86)80336-9

    authors： Lahajnar G,Rupprecht A

    更新日期：1986-11-26 00:00:00

  • Partial characterization of guinea pig cholinephosphotransferase cDNA.

    abstract：:An alternative molecular biology strategy is needed to characterize cholinephosphotransferase (CPT) gene because of the complexity of the problem associated with the solubilization of the membrane-bound enzyme without denaturation. We have synthesized five heterologous oligonucleotide probes based on the published yea...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7862

    authors： Das SK,Beg O,Mukherjee S

    更新日期：1997-12-18 00:00:00

  • Resveratrol stimulates the proliferation and differentiation of osteoblastic MC3T3-E1 cells.

    abstract：:Nutritional and pharmacological factors are needed to prevent bone loss that occurs with increasing age. The chemical compounds that act on bone metabolism as nutrients in food, however, are poorly understood. The effect of resveratrol, a natural phytoestrogen, on the proliferation and differentiation of osteoblastic ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9870

    authors： Mizutani K,Ikeda K,Kawai Y,Yamori Y

    更新日期：1998-12-30 00:00:00

  • Neurotrophic effect of Semaphorin 4D in PC12 cells.

    abstract：:Semaphorins provide crucial attractive and repulsive cues involved in axon guidance during neural development. Out of them, Semaphorin 4D (Sema4D) is enriched in the nervous and immune tissues, and acts as proliferative and survival factors of peripheral lymphocytes in the immune system, but is poorly understood in th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)03023-1

    authors： Fujioka S,Masuda K,Toguchi M,Ohoka Y,Sakai T,Furuyama T,Inagaki S

    更新日期：2003-02-07 00:00:00

  • MicroRNA-613 represses prostate cancer cell proliferation and invasion through targeting Frizzled7.

    abstract：:A growing number of studies have indicated that microRNAs (miRNAs) are critical regulators of carcinogenesis and cancer progression and may serve as potential therapeutic tools for cancer therapy. Frizzled7 (Fzd7), the most important receptor of the Wnt signaling pathway, is extensively involved in cancer development ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.12.054

    authors： Ren W,Li C,Duan W,Du S,Yang F,Zhou J,Xing J

    更新日期：2016-01-15 00:00:00

  • Retroviral coexpression of thymidylate synthase and dihydrofolate reductase confers fluoropyrimidine and antifolate resistance.

    abstract：:Retroviral gene transfer of dominant selectable markers into hematopoietic cells can be used to select genetically modified cells in vivo or to attenuate the toxic effects of chemotherapeutic agents. We show that retroviral gene transfer of thymidylate synthase (TS) confers resistance to TS directed anticancer agents ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.8059

    authors： Fantz CR,Shaw D,Moore JG,Spencer HT

    更新日期：1998-02-04 00:00:00

  • Molecular and physiological characterization of an invertebrate homologue of a calcitonin-related receptor.

    abstract：:Calcitonin is a key hormone involved in the regulation of calcium metabolism in vertebrates. Using oligonucleotide primers derived from consensus sequences of vertebrate calcitonin receptors, we have cloned and characterized the first representative of an invertebrate calcitonin receptor from the bivalve mollusc Crass...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.09.116

    authors： Dubos MP,Badariotti F,Rodet F,Lelong C,Favrel P

    更新日期：2003-10-24 00:00:00

  • Semaphorin-3F attracts the growth cone of cerebellar granule cells through cGMP signaling pathway.

    abstract：:Growth cone extension is guided by extracellular factors during the brain development but the underlying cellular mechanisms remain largely unclear. Here, we examined the potential function of class-3 semaphorins in cultured cerebellar granule cells. We found neuropilin-2 (NP2), the high-affinity receptor for semaphor...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.03.073

    authors： Ding S,Luo JH,Yuan XB

    更新日期：2007-05-18 00:00:00

  • Inhibition of CCl4 metabolism by oxygen varies between isoenzymes of cytochrome P-450.

    abstract：:Oxygen inhibition of CCl4 metabolism by different isoenzymes of cytochrome P-450 was assessed by studying liver microsomes isolated from control rats and rats treated with phenobarbital or isoniazid. Rates of CCl4 metabolism were similar for all microsomes under a nitrogen atmosphere. An air atmosphere inhibited metab...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80450-9

    authors： Burk RF,Hill KE,Lane JM

    更新日期：1988-05-16 00:00:00

  • Development of xeno-free epithelial differentiation media for adherent, non-expanded adipose stromal vascular cell cultures.

    abstract：INTRODUCTION:Reconstruction of respiratory epithelium is critical for the fabrication of bioengineered airway implants. Epithelial differentiation is typically achieved using bovine pituitary extract (BPE). Due to the xenogenic nature and undefined composition of BPE, an alternative for human clinical applications, dev...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.08.104

    authors： Hintze JM,Tchoukalova YD,Sista R,Shah MK,Zhang N,Lott DG

    更新日期：2018-09-18 00:00:00

  • The splicing factor SRSF1 modulates pattern formation by inhibiting transcription of tissue specific genes during embryogenesis.

    abstract：:Alternative splicing is a major mechanism regulating pattern of gene expression through the production of multiple mRNAs from a single gene transcript. Any misregulation can cause various human diseases and also have severe effects on embryogenesis. SRSF1 is one of the critical factors regulating alternative splicing ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.07.021

    authors： Lee SH,Lee HK,Kim C,Kim YK,Ismail T,Jeong Y,Park K,Park JW,Kwon OS,Kang BS,Lee DS,Park MJ,Park DS,Lee HS

    更新日期：2016-09-02 00:00:00

  • Adenosine induces apoptosis through TNFR1/RIPK1/P38 axis in colon cancer cells.

    abstract：:Adenosine, a metabolite of ATP, ubiquitously exists in a wide range of organs and tissues. We previously reported that adenosine was implicated in apoptosis in many cancer cells by extrinsic and/or intrinsic pathways. Here, we found that adenosine suppresses the cell growth by induction of apoptosis of human colonic c...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.03.103

    authors： Yu S,Hou D,Chen P,Zhang Q,Lv B,Ma Y,Liu F,Liu H,Song EJ,Yang D,Liu J

    更新日期：2015-05-08 00:00:00

  • Oral administration of l-ornithine increases the content of both collagen constituting amino acids and polyamines in mouse skin.

    abstract：:l-Ornithine is found in animals as a free amino acid and is a vital component of the urea cycle in the liver; it is reported to have various functions such as promoting wound healing, promoting growth hormone secretion, hypnotic effects, and so on. The present study aimed to investigate the effects of a single oral ad...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.03.147

    authors： Harada D,Nagamachi S,Aso K,Ikeda K,Takahashi Y,Furuse M

    更新日期：2019-05-14 00:00:00

  • Characterization and functional analysis of a novel double-guide C/D box snoRNA in the fission yeast.

    abstract：:Ribose methylation of eukaryotic rRNA is directed by box C/D small nucleolar RNAs (snoRNAs), which pinpoint the nucleotide to be methylated in specific position within the rRNA sequence. Here, we report the identification of a novel double-guide C/D box snoRNA termed snR88 that directs methylation of two previously un...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.12.207

    authors： Bi YZ,Qu LH,Zhou H

    更新日期：2007-03-02 00:00:00

  • Identification of tick periviscerokinin, the first neurohormone of Ixodidae: single cell analysis by means of MALDI-TOF/TOF mass spectrometry.

    abstract：:The first peptidergic neurohormone from the ticks Ixodes ricinus and Boophilus microplus has been identified by using a combination of immunocytochemistry and mass spectrometric analysis of single cells. The novel peptide (Ixori-PVK, PALIPFPRV-NH2) shows a high sequence homology with members of the insect periviscerok...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.10.165

    authors： Neupert S,Predel R,Russell WK,Davies R,Pietrantonio PV,Nachman RJ

    更新日期：2005-12-30 00:00:00

  • Identification of a common microdeletion cluster in 7q21.3 subband among patients with myeloid leukemia and myelodysplastic syndrome.

    abstract：:Monosomy 7 and interstitial deletions in the long arm of chromosome 7 (-7/7q-) is a common nonrandom chromosomal abnormality found frequently in myeloid disorders including acute myeloid leukemia (AML), myelodysplastic syndrome (MDS), and juvenile myelomonocytic leukemia (JMML). Using a short probe-based microarray co...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.04.004

    authors： Asou H,Matsui H,Ozaki Y,Nagamachi A,Nakamura M,Aki D,Inaba T

    更新日期：2009-05-29 00:00:00

  • Suppression of Npr1, not Npr2 gene function induces hypertrophic growth in H9c2 cells in vitro.

    abstract：:Npr1 gene (coding for NPR-A) and Npr2 gene (coding for NPR-B) are identified as intrinsic anti-hypertrophic genes that opposes abnormal cardiac remodeling. However, the functional role of Npr1 and Npr2 genes during cardiac hypertrophic growth is not well understood. Hence, the present investigation was aimed to study ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.07.123

    authors： Manivasagam S,Vellaichamy E

    更新日期：2017-09-16 00:00:00

  • Unusual biphasic melting behaviour of Rhodotorula gracilis DNA.

    abstract：:Ultraviolet, fluorescence and CD spectral analysis suggested unusual structural features of Rhodotorula gracilis ATCC 90950 DNA. R. gracilis DNA exhibited 13% hyperchromicity at 260 nm as against 26% shown by calf thymus DNA. The biphasic melting curve, one phase between 88-92 degrees C and the other between 92-97 deg...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1907

    authors： Vishnuvardhan D,Divakar S,Joseph R

    更新日期：1995-06-26 00:00:00

  • Self-association of the hepatitis B virus X protein in the yeast two-hybrid system.

    abstract：:Self-association of the transactivator HBx protein of hepatitis B virus was investigated using the yeast two-hybrid system. Expression vectors for the full-length HBx (X0) and its truncated mutants (X15 and X16) were constructed by separately ligating the DNA-binding (BD) and transactivation domains (AD) of Gal4. Co-t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.03.140

    authors： Reddi HV,Kumar V

    更新日期：2004-05-14 00:00:00

  • Two genetically different MU-NANA neuraminidases in human leucocytes.

    abstract：:Human leucocytes contain two different MU-NANA neuraminidases, which can be distinguished by Concanavalin A binding. The Con A binding form is predominant in lymphocytes (more than 80%) and the non-binding form predominates in granulocytes. The pH optima of both these neuraminidases as well as their subcellular locali...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91224-x

    authors： Verheijen FW,Janse HC,van Diggelen OP,Bakker HD,Loonen MC,Durand P,Galjaard H

    更新日期：1983-12-16 00:00:00

  • Surface plasmon resonance for measuring TBP-promoter interaction.

    abstract：:A procedure was developed for real-time measurement of the interaction between an archaeal TATA-binding protein (TBP) with stress-gene promoters from the archaeon Methanosarcina mazeii using surface plasmon resonance (SPR), the BIACORE 3000 equipment, and the SA (streptavidin) Sensor Chip. Measurements were based on t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02388-4

    authors： Conway de Macario E,Rudofsky UH,Macario AJ

    更新日期：2002-11-15 00:00:00