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Inhibition, tissue distribution and hormonal specificity of a promoter-binding factor for the uteroglobin gene.

Abstract：

:We had earlier reported a uteroglobin promoter-binding factor in nuclei from progesterone-stimulated rabbit endometrium that was inhibited by a factor in nuclei without progesterone stimulation or from non-target tissues (Rider, V., and Bullock, D.W., 1988. Biochem. Biophys. Res. Comm. 156, 1368-1375). In the course of purification of the inhibitory activity, the effect was shown to be due to contaminating genomic DNA. The inhibitor was destroyed by treatment with DNase I and resisted phenol-chloroform extraction. Fractionation of nuclear extracts on columns of DEAE-Sepharose separated the inhibitor and revealed the presence of binding activity in unstimulated or estrogen-treated endometrium, as well as in liver, lung and ovary. The tissue and hormonal specificity of the promoter binding factor is thus less restricted than recently reported.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Daniel PB,Molloy C,Rayner JC,Hill AM,Bullock DW

doi

10.1006/bbrc.1994.2455

subject

Has Abstract

pub_date

1994-10-14 00:00:00

pages

273-7

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(84)72455-7

journal_volume

204

pub_type

杂志文章

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