Abstract:
:The recently detected Rieske iron-sulfur center in the membrane of the thermoacidophilic archaeon Sulfolobus acidocaldarius (Anemüller et al., 1993, FEBS Lett. 318, 61-64) was further characterized by EPR spectroscopy, coupled to redox-potentiometry and functional studies. The reduction potential is pH-dependent above pH 6, revealing the influence of two ionization equilibria in the oxidized form, with pKaox-values of 6.2 and 8.5. Above pH 9, the slope of the curve is--120 mV/pH-unit. A partially purified fraction exerted a ubiquinol-cytochrome c oxidoreductase activity. To our knowledge, for the first time, in a membrane bound Rieske iron-sulfur protein, unequivocal evidence for a two proton dependent redox equilibrium is presented.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Anemüller S,Schmidt CL,Schäfer G,Bill E,Trautwein AX,Teixeira Mdoi
10.1006/bbrc.1994.1920subject
Has Abstractpub_date
1994-07-15 00:00:00pages
252-7issue
1eissn
0006-291Xissn
1090-2104pii
S0006-291X(84)71920-6journal_volume
202pub_type
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