Effect of advanced glycation end products on lens epithelial cells in vitro.

abstract：:Peroxisome proliferator-activated receptors (PPARs) have been considered to be desirable targets for metabolic syndrome, even though their specific agonists have several side effects including body weight gain, edema and tissue failure. Previously, we have reported in vitro effects of Sargassum yezoense (SY) and its i...

journal_title：Biochemical and biophysical research communications

authors： Kim SN,Lee W,Bae GU,Kim YK

更新日期：2012-08-10 00:00:00

abstract：:Tropomyosins are a family of actin binding proteins encoded by a group of highly conserved genes. Humans have four tropomyosin-encoding genes: TPM1, TPM2, TPM3, and TPM4, each of which is known to generate multiple isoforms by alternative splicing, promoters, and 3' end processing. TPM1 is the most versatile and encod...

journal_title：Biochemical and biophysical research communications

authors： Denz CR,Narshi A,Zajdel RW,Dube DK

更新日期：2004-08-06 00:00:00

abstract：:Oriented fibers of DNA, prepared by the wet spin method, were imaged by atomic force microscopy. It was found that an oriented fiber's substructure is an array of assemblages of DNA. For native DNA, the assemblages exhibit a characteristic width of 76 nm, a thickness of 20 nm, and appear to carry a right handed twist....

journal_title：Biochemical and biophysical research communications

authors： Rampino NJ

更新日期：1992-01-15 00:00:00

abstract：:Inducible 6-phosphofructo-2-kinase (iPFK-2; PFKFB3) produces fructose-2,6-bisphosphate (F2,6BP), which is a potent allosteric activator of 6-phosphofructo-1-kinase (PFK-1), the rate-limiting step in glycolysis. iPFK-2 functions as an activator of anaerobic glycolysis within the hypoxic microenvironment of growing tumo...

journal_title：Biochemical and biophysical research communications

authors： Chesney J,Telang S,Yalcin A,Clem A,Wallis N,Bucala R

更新日期：2005-05-27 00:00:00

abstract：:Fanconi anemia (FA) is a genetic disorder characterized by congenital malfunction, bone marrow failure and hypersensitivity to DNA damage. FANCD2 protein play the central role in FA pathway. To study the in vivo role of FANCD2, we generated and characterized a new Fancd2 knockout mouse strain with 7bp deletion in Fanc...

journal_title：Biochemical and biophysical research communications

authors： Yang Q,Xie H,Zhong Y,Li D,Ke X,Ying H,Yu B,Zhang T

更新日期：2019-06-30 00:00:00

abstract：:Endothelin (200 nM) evoked a rapid rise in [Ca2+]i which was then followed by a maintained elevation of [Ca2+]i. The initial transient can be explained by the release of stored Ca2+ whilst the maintained plateau is likely to be an influx of Ca2+ as it was partially inhibited by nifedipine (5 microM) and the remaining ...

journal_title：Biochemical and biophysical research communications

authors： Simpson AW,Ashley CC

更新日期：1989-09-29 00:00:00

abstract：:Studies examining the relationship between cellular sortilin and VLDL-B100 secretion demonstrate inconsistent results. Current studies explore the possibility that discrepancies may be related to insulin sensitivity. McArdle RH7777 cells (McA cells) cultured under serum enriched conditions lose sensitivity to insulin....

journal_title：Biochemical and biophysical research communications

authors： Sparks RP,Guida WC,Sowden MP,Jenkins JL,Starr ML,Fratti RA,Sparks CE,Sparks JD

更新日期：2016-09-16 00:00:00

abstract：:Using a novel approach for analysis of TRPC channel activity, we report here that NSAIDs are involved into regulation of TRPC channels in the podocytes of the freshly isolated decapsulated glomeruli. Fluorescence and electron microscopy techniques confirmed the integrity of podocytes in the glomeruli. Western blotting...

journal_title：Biochemical and biophysical research communications

authors： Ilatovskaya DV,Levchenko V,Ryan RP,Cowley AW Jr,Staruschenko A

更新日期：2011-05-06 00:00:00

abstract：:Two multidrug-resistant human leukemic CCRF-CEM sublines (CEM/VCR R and CEM/VLB100) were significantly more resistant to tetracycline, a hydrophilic antibiotic, than parental cells (P < 0.001). Verapamil and cyclosporin A completely reversed tetracycline resistance in CEM/VCR R cells, which also accumulated and retain...

journal_title：Biochemical and biophysical research communications

authors： Kavallaris M,Madafiglio J,Norris MD,Haber M

更新日期：1993-01-15 00:00:00

abstract：:The eukaryotic DNA cytosine-5-methyltransferase (E.C.2.1.1.37) is known to methylate cytosine in DNA mainly, but not exclusively in C-G. In the present study the minor, non-C-G recognition sequences of a rat DNA methyltransferase were analyzed by Maxam-Gilbert sequencing of in vitro methylated SV40 DNA. The enzyme met...

journal_title：Biochemical and biophysical research communications

authors： Hubrich-Kühner K,Buhk HJ,Wagner H,Kröger H,Simon D

更新日期：1989-05-15 00:00:00

abstract：:The presence of a specific receptor for 1,25-dihydroxyvitamin D (1,25(OH)2D) was investigated in a cell line A7r5 derived from fetal aorta. 1,25(OH)2[3H]D3 binding to cytosol was saturated at 0.6-1 nM, and Scatchard analysis yielded dissociation constant and binding sites, (3.02 +/- 0.4) X 10(-11) M and 33.9 +/- 5.8 f...

journal_title：Biochemical and biophysical research communications

authors： Kawashima H

更新日期：1987-07-15 00:00:00

abstract：:Transmissible gastroenteritis virus (TGEV), an enteropathogenic coronavirus, causes severe lethal watery diarrhea and dehydration in piglets. Previous studies indicate that TGEV infection induces cell apoptosis in host cells. In this study, we investigated the roles and regulation of reactive oxygen species (ROS) in T...

journal_title：Biochemical and biophysical research communications

authors： Ding L,Zhao X,Huang Y,Du Q,Dong F,Zhang H,Song X,Zhang W,Tong D

更新日期：2013-12-06 00:00:00

abstract：:Oxytocin binding activity of myoepithelial cell membranes from mammary tissue was measured under a variety of different experimental conditions. Mammary tissue from non-lactating rats bound oxytocin with a Kd of 9.2 +/- 1.6 nM (+/- S.E.) and indicates that receptors are retained by the myoepithelial cells in a non-lac...

journal_title：Biochemical and biophysical research communications

authors： Ruberti A,Olins GM,Eakle KA,Bremel RD

更新日期：1983-04-29 00:00:00

abstract：:PHF8 is a JmjC domain-containing protein and erases repressive histone marks including H4K20me1 and H3K9me1/2. It binds to H3K4me3, an active histone mark usually located at transcription start sites (TSSs), through its plant homeo-domain, and is thus recruited and enriched in gene promoters. PHF8 is involved in the d...

journal_title：Biochemical and biophysical research communications

authors： Shen Y,Pan X,Zhao H

更新日期：2014-08-15 00:00:00

abstract：:TRPA1 of insects and several tetrapod vertebrates except for those of rodents have been reported to be activated by noxious chemicals and also by high temperature with a relatively clear threshold. We previously analyzed the characteristics of two TRPA1 paralogs of zebrafish (zTRPA1a, b) and demonstrated that zTRPA1a ...

journal_title：Biochemical and biophysical research communications

authors： Oda M,Saito K,Hatta S,Kubo Y,Saitoh O

更新日期：2017-12-09 00:00:00

abstract：:Aqueous extracts of cigarette tar produce hydroxyl radicals that are spin trapped by 5,5-dimethyl-1-pyrroline-N-oxide. The addition of catalase almost completely inhibits and superoxide dismutase partially inhibits spin adduct formation. The addition of ethylenediamine tetraacetic acid greatly increases the amount of ...

journal_title：Biochemical and biophysical research communications

authors： Cosgrove JP,Borish ET,Church DF,Pryor WA

更新日期：1985-10-15 00:00:00

abstract：:This study examines the effect of TNFα on whole bovine intervertebral discs in organ culture and its association with changes characteristic of intervertebral disc degeneration (IDD) in order to inform future treatments to mitigate the chronic inflammatory state commonly found with painful IDD. Pro-inflammatory cytoki...

journal_title：Biochemical and biophysical research communications

authors： Purmessur D,Walter BA,Roughley PJ,Laudier DM,Hecht AC,Iatridis J

更新日期：2013-03-29 00:00:00

abstract：:Oxidative stress is involved in a multitude of pathological conditions. In the present study, cell cycle arrest was demonstrated in monolayer cultures of Chinese hamster ovary cells subjected to low-level oxidative stress induced by the addition of hydrogen peroxide. Fluorescence-activated cell sorting analysis charac...

journal_title：Biochemical and biophysical research communications

authors： Clopton DA,Saltman P

更新日期：1995-05-05 00:00:00

abstract：:Two common non-steroidal anti-inflammatory drugs (NSAIDs) and their nitric oxide (NO) adducts were evaluated for effects on stomach and thymus. Following 4-h duration (acute) oral dosing of fasted male Wistar rats, 1.33 x 10(-4)mol/kg of ibuprofen caused significant visual irritation score and microscopic thinning, al...

journal_title：Biochemical and biophysical research communications

authors： Downing JE,Madden JC,Ingram MJ,Rostron C

更新日期：2005-08-26 00:00:00

abstract：:Erythropoietin has been thought to be secreted to plasma soon after the production because of the difficulty of Western blot analysis and immunohistochemistry. We established the new methods of Western blot analysis and immunohistochemistry. Using the new methods, we investigated the effects of aldosterone and fludroc...

journal_title：Biochemical and biophysical research communications

authors： Yasuoka Y,Izumi Y,Nagai T,Fukuyama T,Nakayama Y,Inoue H,Horikawa K,Kimura M,Nanami M,Yanagita K,Oshima T,Yamazaki T,Uematsu T,Yamamura R,Kobayashi N,Shimada Y,Nagaba Y,Nakanishi T,Yamashita T,Mukoyama M,Sato Y,K

更新日期：2018-09-18 00:00:00

abstract：:Contact between an adherent cell and the extracellular matrix (ECM) promotes the recruitment of structural and signaling molecules to the cytoplasmic domain of integrins, which mediate cell adhesion, cell migration, and cell growth. It is unclear whether the intracellular recruitment of these cytoplasmic molecules enh...

journal_title：Biochemical and biophysical research communications

authors： Rahman A,Tseng Y,Wirtz D

更新日期：2002-08-23 00:00:00

abstract：:The tumor suppressor, RASSF2 (Ras association domain family 2), is frequently downregulated in a number of cancers. Although exogenously expressed RASSF2 induces apoptotic cell death, the precise roles of RASSF2 under pro-apoptotic conditions remain largely unknown. Here, we demonstrate that MST1 (mammalian sterile 20...

journal_title：Biochemical and biophysical research communications

authors： Song H,Oh S,Oh HJ,Lim DS

更新日期：2010-01-01 00:00:00

abstract：:Glial precursor cells (GPCs) are present in the adult human central nervous system (CNS) and they can be isolated and maintained in culture for in vitro studies. This study analysed expression of mGluR3 and mGluR5 metabotropic glutamate receptor (mGluR) mRNAs in GPCs. A2B5 surface antigen positive GPCs were isolated u...

journal_title：Biochemical and biophysical research communications

authors： Luyt K,Varadi A,Halfpenny CA,Scolding NJ,Molnar E

更新日期：2004-06-18 00:00:00

abstract：:Acyl-CoA dehydrogenases (ACADs) are a family of mitochondrial enzymes catalyzing the initial rate-limiting step in the beta-oxidation of fatty acyl-CoA. The reaction provides main source of energy for human heart and skeletal muscle. Eight human ACADs have been described. Deficiency of these enzymes, especially very l...

journal_title：Biochemical and biophysical research communications

authors： Zhang J,Zhang W,Zou D,Chen G,Wan T,Zhang M,Cao X

更新日期：2002-10-04 00:00:00

abstract：:Several fusion proteins of mouse Interleukins (mILs) and the enhanced green fluorescent protein (EGFP) were expressed in fibroblast and epithelial cells. Among these proteins, the mIL-31 derivative was the most efficiently secreted into the medium in a N-glycosylation-dependent manner. From the analysis of deletion mu...

journal_title：Biochemical and biophysical research communications

authors： Akiyama N,Ohno Y,Fukuda T,Manome Y,Saito S

更新日期：2009-04-17 00:00:00

abstract：:Liver X receptors LXRα (NR1H3) and LXRβ (NR1H2) are transcription factors belonging to the nuclear receptor superfamily, activated by specific oxysterols, oxidized derivatives of cholesterol. These receptors are involved in the regulation of testis physiology. Lxr-deficient mice pointed to the physiological roles of t...

journal_title：Biochemical and biophysical research communications

authors： Oumeddour A,Viennois E,Caira F,Decourbey C,Maqdasy S,Tahraoui A,Baron S,Volle DH,Lobaccaro JM

更新日期：2014-04-11 00:00:00

abstract：:Previously, we have reported that two clustered cat genes from Acenitobacter lwoffi K24 had different arrangements, catB1C1A1 and catB2A2C2 (Kim, S.I., S.-H. Leem, J.-S. Choi, Y.H. Chung, S. Kim, Y.-M. Park, Y.K. Park, Y.N. Lee, and K.-S. Ha. 1997, J. Bacteriol. 179, 5226-5231). By further analysis of the organization...

journal_title：Biochemical and biophysical research communications

authors： Kim SI,Leem SH,Choi JS,Ha KS

更新日期：1998-02-04 00:00:00

abstract：:We have previously shown that the transcription factor C/EBP delta is involved in the intestinal inflammatory response. C/EBP delta regulates several inflammatory response genes, such as haptoglobin, in the rat intestinal epithelial cell line IEC-6 in response to IL-1. However, the different C/EBP delta domains involv...

journal_title：Biochemical and biophysical research communications

authors： Svotelis A,Doyon G,Bernatchez G,Désilets A,Rivard N,Asselin C

更新日期：2005-03-11 00:00:00

abstract：:We have previously reported that TTF-1, a homeodomain-containing transcription factor, regulates circadian rhythm of pituitary adenylate cyclase-activating polypeptide gene expression in the rat hypothalamus. In this study we found that TTF-1 mRNA was specifically expressed in the rat suprachiasmatic nucleus (SCN) and...

journal_title：Biochemical and biophysical research communications

authors： Son YJ,Yun CH,Kim JG,Park JW,Kim JH,Kang SG,Lee BJ

更新日期：2009-03-13 00:00:00

abstract：:Tissue nicotinamide adenine dinucleotide (NAD+) decline has been implicated in aging. We have recently identified CD38 as a central regulator involved in tissue NAD+ decline during the aging process. CD38 is an ecto-enzyme highly expressed in endothelial and inflammatory cells. To date, the mechanisms that regulate CD...

journal_title：Biochemical and biophysical research communications

authors： Chini C,Hogan KA,Warner GM,Tarragó MG,Peclat TR,Tchkonia T,Kirkland JL,Chini E

更新日期：2019-05-28 00:00:00