Non-C-G recognition sequences of DNA cytosine-5-methyltransferase from rat liver.

abstract：:cGMP-Phosphodiesterase 6 (PDE6) is the central effector enzyme in the phototransduction system of vertebrate photoreceptors. We have recently found that PDE6 accumulates in a detergent-resistant membrane (DRM) fraction in response to excitation of bovine rod phototransduction system. Here, we studied the molecular mec...

journal_title：Biochemical and biophysical research communications

authors： Liu H,Seno K,Hayashi F

更新日期：2003-03-28 00:00:00

abstract：:The aggregation state of the gonadotropin receptor has been examined by coupling fluorescence energy transfer donor and acceptor fluorophores to hCG and LH. Energy transfer is observed at low (4 degrees C) but not at high (37 degrees C) temperature. Energy transfer could also be detected with receptor solubilized in t...

journal_title：Biochemical and biophysical research communications

authors： Harmatz D,Ji TH,Middaugh CR

更新日期：1985-03-15 00:00:00

abstract：:Overexpression of AtNTRC (AtNTRC(OE)) in Arabidopsis thaliana led to a freezing and cold stress tolerance, whereas a knockout mutant (atntrc) showed a stress-sensitive phenotype. Biochemical analyses showed that the recombinant AtNTRC proteins exhibited a cryoprotective activity for malate dehydrogenase and lactic deh...

journal_title：Biochemical and biophysical research communications

authors： Moon JC,Lee S,Shin SY,Chae HB,Jung YJ,Jung HS,Lee KO,Lee JR,Lee SY

更新日期：2015-08-07 00:00:00

abstract：:The cellular molecular motor kinesin-1 mediates the microtubule-dependent transport of a range of cargo. We have previously identified an interaction between the cargo-binding domain of kinesin-1 heavy chain KIF5B and the membrane-associated SNARE proteins SNAP-25 and SNAP-23. In this study we further defined the mini...

journal_title：Biochemical and biophysical research communications

authors： Morton AM,Cunningham AL,Diefenbach RJ

更新日期：2010-01-01 00:00:00

abstract：:By DNA sequence analysis, we have determined a spectrum of 61 spontaneous mutations occurring in the endogenous tonB gene in the polA1 strain of Escherichia coli. The overall mutation frequency was approximately 2.4-fold higher in the polA1 strain and this was attributable to enhanced rates of deletion and frameshift ...

journal_title：Biochemical and biophysical research communications

authors： Agemizu Y,Uematsu N,Yamamoto K

更新日期：1999-08-11 00:00:00

abstract：:Fibrinogen deposition and smooth muscle cell migration are important causes of atherosclerosis and angiogenesis. Involvement of calpains in vascular smooth muscle cell adhesion onto fibrinogen was investigated. Using calpain inhibitors, we showed that activation of calpains was required for smooth muscle cell spreadin...

journal_title：Biochemical and biophysical research communications

authors： Paulhe F,Bogyo A,Chap H,Perret B,Racaud-Sultan C

更新日期：2001-11-09 00:00:00

abstract：:The effects of pioglitazone, a novel antidiabetic insulin-sensitizing agent, on insulin signaling in skeletal muscles from genetically obese Wistar fatty rats and their lean littermates were studied. Increased tyrosine phosphorylation levels of insulin receptors (IRs) and insulin receptor substrate 1 (IRS-1) in respon...

journal_title：Biochemical and biophysical research communications

authors： Hayakawa T,Shiraki T,Morimoto T,Shii K,Ikeda H

更新日期：1996-06-14 00:00:00

abstract：:We have examined the kinetics of the effects of inhibitors of the Na+/H(+)-antiporter (dimethylamiloride) and the vacuolar H(+)-ATPase (bafilomycin A1) on bone resorption by disaggregated rat osteoclasts in the bone slice assay. Bafilomycin A1 (100 nM) inhibited resorption by approximately 95%, 75%, 80% and 60% respec...

journal_title：Biochemical and biophysical research communications

authors： Hall TJ,Schaeublin M,Chambers TJ

更新日期：1992-11-16 00:00:00

abstract：:Inflammatory mediators and adhesion molecules have been implicated in a variety of diseases including atherosclerosis. As both the mediator-releasing and targeted cells, vascular endothelial cells play key role in pathological processes. NF-κB signaling regulates a cluster of inflammatory factors in LPS-activated vasc...

journal_title：Biochemical and biophysical research communications

authors： Yu S,Chen X,Xiu M,He F,Xing J,Min D,Guo F

更新日期：2017-03-25 00:00:00

abstract：:Using the whole-cell and single channel recording techniques, the influence of actin cytoskeletons on L-type Ca2+ current was investigated in human gastric smooth muscle cells. In isotonic condition, an actin depolymerizer cytochalasin D (Cyt-D) markedly decreased the whole-cell current (I(Ba)) without changing steady...

journal_title：Biochemical and biophysical research communications

authors： Kim JH,Rhee PL,Kang TM

更新日期：2007-01-12 00:00:00

abstract：:Alzheimer's disease (AD) is a progressive neurodegenerative disorder and the most common cause of dementia. Today, AD can be diagnosed with certainty only post-mortem, by histopathologic staining of Abeta plaques and neurofibrillary tangles in brain tissue sections. We have developed an ultra-sensitive assay potential...

journal_title：Biochemical and biophysical research communications

authors： Funke SA,Birkmann E,Henke F,Görtz P,Lange-Asschenfeldt C,Riesner D,Willbold D

更新日期：2007-12-28 00:00:00

abstract：:The secondary structure of human pancreatic thread protein (HPTP) in solution at acid pH was derived using Fourier transform infrared (FT-IR) and laser Raman spectroscopic studies. The experimentally derived secondary structure of HPTP was compared with the secondary structure obtained by the Chou-Fasman algorithm. Pa...

journal_title：Biochemical and biophysical research communications

authors： Renugopalakrishnan V,Dobbs JC,Collette TW,Carreira LA,Hutson TB,Garduño-Juarez R

更新日期：1999-05-19 00:00:00

abstract：:The 32/20-kDa two-chain form of protective protein/cathepsin A (CathA) secreted by human platelets was thermostable in the aggregation supernatant at acidic pH, but was denatured at neutral pH. Leupeptin partly protected the CathA against denaturation, which was not observed in the supernatant after depletion of the c...

journal_title：Biochemical and biophysical research communications

authors： Itoh K,Naganawa Y,Kamei S,Shimmoto M,Sakuraba H

更新日期：1998-12-18 00:00:00

abstract：:In Wernicke's encephalopathy and thiamine deficiency (TD), the cause of this brain disorder, development of inflammation is an important aspect of the disease process. How this pathological mechanism relates to the neurologic impairment associated with TD, however, remains unclear. A key feature of the inflammatory pr...

journal_title：Biochemical and biophysical research communications

authors： Wang D,Hazell AS

更新日期：2010-11-05 00:00:00

abstract：:Ophiopogonin D (OPD) is the chief pharmacological active component of the traditional Chinese herbal prescription drug-Shenmai injection (SMI), which has been used to prevent and treat cardiovascular diseases. In the present study, we investigated whether OPD protectively relieve cardiac hypertrophy against inflammati...

journal_title：Biochemical and biophysical research communications

authors： Wang Y,Huang X,Ma Z,Wang Y,Chen X,Gao Y

更新日期：2018-09-05 00:00:00

abstract：:18β-Glycyrrhetinic acid (18β-GA) obtained from the herb liquorice has various pharmacological properties including anti-inflammatory and anti-bacterial activities. However, potential biological anti-obesity activities are unclear. In this study, novel biological activities of 18β-GA in the adipogenesis of 3T3-L1 pread...

journal_title：Biochemical and biophysical research communications

authors： Moon MH,Jeong JK,Lee YJ,Seol JW,Ahn DC,Kim IS,Park SY

更新日期：2012-04-20 00:00:00

abstract：:Despite their advantages in analysis, 4D NMR experiments are still infrequently used as a routine tool in protein NMR projects due to the long duration of the measurement and limited digital resolution. Recently, new acquisition techniques for speeding up multidimensional NMR experiments, such as nonlinear sampling, i...

journal_title：Biochemical and biophysical research communications

authors： Shigemitsu Y,Ikeya T,Yamamoto A,Tsuchie Y,Mishima M,Smith BO,Güntert P,Ito Y

更新日期：2015-02-06 00:00:00

abstract：:Phosphorylation of HMG 14 proteins from calf thymus and avian erythrocytes was studied using a cyclic GMP-dependent protein kinase from bovine lung. HMG 14 from calf thymus was a good substrate for the enzyme, but HMG 14 from avian erythrocytes was not phosphorylated. Of the potential phosphorylation sites, the one in...

journal_title：Biochemical and biophysical research communications

authors： Palvimo J,Linnala-Kankkunen A,Mäenpää PH

更新日期：1983-01-27 00:00:00

abstract：:We have studied the importance of charge and hydrogen-bonding potential of the phosphodiester backbone for binding and cleavage by EcoRI restriction endonuclease. We used 12-mer oligodeoxynucleotide substrates with single substitutions of phosphates by chiral methylphosphonates at each position of the recognition sequ...

journal_title：Biochemical and biophysical research communications

authors： Rosati O,Srivastava TK,Katti SB,Alves J

更新日期：2002-07-05 00:00:00

abstract：:The x-ray absorption near edge structure (XANES) spectra of hemoglobin and myoglobin have been measured at the wiggler beam line of the Frascati Synchrotron Radiation Facility. The energy shifts of the iron absorption jump edge and the chemical shifts of the bound excited state at threshold of 1s core excitations, goi...

journal_title：Biochemical and biophysical research communications

authors： Bianconi A,Congiu-Castellano A,Dell'Ariccia M,Giovannelli A,Burattini E,Durham PJ

更新日期：1985-08-30 00:00:00

abstract：:Ambroxol is often used as a mucolytic agent in various lung diseases. However, it is unclear how ambroxol acts on bronchial epithelial cells. To clarify the action of ambroxol, we studied the effects of ambroxol on the ion transport in human Calu-3 cells, a human submucosal serous cell line, measuring the transepithel...

journal_title：Biochemical and biophysical research communications

authors： Hasegawa I,Niisato N,Iwasaki Y,Marunaka Y

更新日期：2006-05-05 00:00:00

abstract：:Internal backbone dynamic motions are essential for different protein functions and occur on a wide range of time scales, from femtoseconds to seconds. Molecular dynamic (MD) simulations and nuclear magnetic resonance (NMR) spin relaxation measurements are valuable tools to gain access to fast (nanosecond) internal mo...

journal_title：Biochemical and biophysical research communications

authors： Liu Q,Shi C,Yu L,Zhang L,Xiong Y,Tian C

更新日期：2015-02-13 00:00:00

abstract：:Discerning oncogenic drivers from passengers remains a major effort in understanding of the essence of the initiation and development of hepatocellular carcinoma (HCC), the most common primary liver malignancy and the third leading cause of cancer mortality worldwide. Here we report that MTA2, Metastasis Associated 1 ...

journal_title：Biochemical and biophysical research communications

authors： Guan C,Chang Z,Gu X,Liu R

更新日期：2019-07-12 00:00:00

abstract：:NF-kappaB plays an important role in oncogenesis. Recently, we have demonstrated that loss of p53 function enhances DNA binding and transcriptional activities of NF-kappaB via IKKalpha and IKKbeta, and that glycolysis, activated by NF-kappaB, has an integral role in oncogene-induced cell transformation. Here, we show ...

journal_title：Biochemical and biophysical research communications

authors： Kawauchi K,Araki K,Tobiume K,Tanaka N

更新日期：2008-07-18 00:00:00

abstract：:The study of [3H]-uridine uptake by mouse peritoneal macrophages showed that this is an active, temperature- and protein synthesis-dependent phenomenon, which is early altered when are exposed to a variety of stimuli. Murine recombinant interferon-gamma, a stimulus able to activate macrophage and to induce the product...

journal_title：Biochemical and biophysical research communications

authors： Busolo F,Conventi L,Gatti C,Monti D,Troiano L,Franceschi C

更新日期：1989-04-14 00:00:00

abstract：:Yeast cells adapted to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) exhibit a plasma membrane less susceptible to 2,4-D-induced disruption and are more tolerant than unadapted cells to lethal concentrations of the herbicide. These cells, adapted to grow in the presence of increasing concentrations of 2,4-D, we...

journal_title：Biochemical and biophysical research communications

authors： Viegas CA,Cabral MG,Teixeira MC,Neumann G,Heipieper HJ,Sá-Correia I

更新日期：2005-04-29 00:00:00

abstract：:We have recently reported that mutant but not wild-type (wt) p53 protein was ADP-ribosylated in primary rat cells overexpressing the temperature-sensitive murine p53val135 gene. To examine whether the lack of susceptibility to modification is a specific feature of p53val135 adopting wt conformation or rather a general...

journal_title：Biochemical and biophysical research communications

authors： Wesierska-Gadek J,Schmid G,Cerni C

更新日期：1996-07-05 00:00:00

abstract：:We report the identification of the full-length cDNA for a novel ATP-binding cassette (ABC) transporter from human macrophages. The mRNA is of 6.8 kb size and contains an open reading frame encoding a polypeptide of 2146 amino acids with a calculated molecular weight of 220 kDa. The predicted protein product is compos...

journal_title：Biochemical and biophysical research communications

authors： Kaminski WE,Orsó E,Diederich W,Klucken J,Drobnik W,Schmitz G

更新日期：2000-07-05 00:00:00

abstract：:Fanconi anemia (FA) is a genetic disorder characterized by congenital malfunction, bone marrow failure and hypersensitivity to DNA damage. FANCD2 protein play the central role in FA pathway. To study the in vivo role of FANCD2, we generated and characterized a new Fancd2 knockout mouse strain with 7bp deletion in Fanc...

journal_title：Biochemical and biophysical research communications

authors： Yang Q,Xie H,Zhong Y,Li D,Ke X,Ying H,Yu B,Zhang T

更新日期：2019-06-30 00:00:00

abstract：:Porphyromonas gingivalis acquires heme through an outer-membrane heme transporter HmuR and heme-binding hemophore-like lipoprotein HmuY. Here, we compare binding of iron(III) mesoporphyrin IX (mesoheme) and iron(III) deuteroporphyrin IX (deuteroheme) to HmuY with that of iron(III) protoporphyrin IX (protoheme) and pro...

journal_title：Biochemical and biophysical research communications

authors： Wojaczyński J,Wójtowicz H,Bielecki M,Olczak M,Smalley JW,Latos-Grażyński L,Olczak T

更新日期：2011-07-29 00:00:00