DNA sequence analysis of spontaneous tonB deletion mutations in a polA1 strain of Escherichia coli K12.

abstract：:The alpha- and/or beta-subunits of human beta-hexosaminidase A (alphabeta) and B (betabeta) are approximately 60% identical. In vivo only beta-hexosaminidase A can utilize GM2 ganglioside as a substrate, but requires the GM2 activator protein to bind GM2 ganglioside and then interact with the enzyme, placing the termi...

journal_title：Biochemical and biophysical research communications

authors： Zarghooni M,Bukovac S,Tropak M,Callahan J,Mahuran D

更新日期：2004-11-19 00:00:00

abstract：:Saccharomyces cerevisiae RecQ helicase (Sgs1) combines with DNA topoisomerase III (Top3) and RecQ-mediated genome instability 1 (Rmi1) to form an evolutionarily conserved complex that is required for processing homologous recombination intermediates and restarting collapsed replication forks. It was previously reporte...

journal_title：Biochemical and biophysical research communications

authors： Lai MS,Seki M,Tada S,Enomoto T

更新日期：2012-10-26 00:00:00

abstract：:pNO40/PS1D, a novel nucleolar protein, has been characterized as a core protein of eukaryotic 60S ribosome and at least two splicing forms of pNO40 mRNAs with alternative starting sites have been identified. Through production of knockout (ko) mice with either exon 2 (△E2), exon 4 (△E4) or △E2+E4 targeted disruption w...

journal_title：Biochemical and biophysical research communications

authors： Lin YM,Wu CC,Chang YC,Wu CH,Ho HL,Hu JW,Chang RC,Wang CT,Ouyang P

更新日期：2016-01-22 00:00:00

abstract：:N1999A2 (NA2) is a new non-protein antitumor antibiotic that contains a stable 9-membered ring enediyne chromophore similar to a neocarzinostatin chromophore (NCS-chr). We have compared DNA cleavage reactions between NA2 and NCS-chr, and also clarified some characteristics of DNA strand scission by NA2. It was found t...

journal_title：Biochemical and biophysical research communications

authors： Miyagawa N,Sasaki D,Matsuoka M,Imanishi M,Ando T,Sugiura Y

更新日期：2003-06-20 00:00:00

abstract：:The effect of HMG1 on the transcriptional block caused by left-handed Z-form in a highly negatively supercoiled DNA was examined using a supercoiled plasmid containing a (CG)10 sequence downstream of promoters. The transcription by E. coli RNA polymerase was blocked at the boundary of alternating CG sequence in the Z-...

journal_title：Biochemical and biophysical research communications

authors： Waga S,Mizuno S,Yoshida M

更新日期：1988-05-31 00:00:00

abstract：:Adenosine is a potent bronchoconstricting agent that is released by activated mast cells and hypoxic lung tissue. However, both inhibition and stimulation of mediator release from human lung mast cells by adenosine have been described, and this discrepancy seems to be due to contaminating cells or the effects of enzym...

journal_title：Biochemical and biophysical research communications

authors： Suzuki H,Takei M,Nakahata T,Fukamachi H

更新日期：1998-01-26 00:00:00

abstract：:Reverse cholesterol transport (RCT) plays an important role in cholesterol and lipid metabolism. Regulating the activities of key transporters and receptors in RCT, such as ATP-binding cassette transporter A1 (ABCA1), helps to prevent atherosclerotic cardiovascular disease. In this study, we used an ABCA1 promoter luc...

journal_title：Biochemical and biophysical research communications

authors： Wang X,Luo J,Li N,Liu L,Han X,Liu C,Zuo X,Jiang X,Li Y,Xu Y,Si S

更新日期：2018-09-26 00:00:00

abstract：RATIONALE:Islet-1 positive (Islet-1(+)) cardiac progenitor cells give rise to the right ventricle, atria and outflow tract during murine cardiac development. In the adult heart Islet-1 expression is limited to parasympathetic neurons, few cardiomyocytes, smooth muscle cells, within the proximal aorta and pulmonary arte...

journal_title：Biochemical and biophysical research communications

authors： Weinberger F,Mehrkens D,Starbatty J,Nicol P,Eschenhagen T

更新日期：2015-01-02 00:00:00

abstract：:Platelet activating factor was isolated from the lesional scale of psoriatic patients using the method described by Bligh and Dyer (8). The extract was subjected to thin layer chromatography, and the region of the plate co-migrating with platelet activating factor removed. A portion of each sample was assayed for aggr...

journal_title：Biochemical and biophysical research communications

authors： Mallet AI,Cunningham FM

更新日期：1985-01-16 00:00:00

abstract：:Protein phosphorylation, governed by kinases and phosphatases, plays a pivotal role in enormous cellular signaling pathways. Although PPP family of serine/threonine phosphatases have been involved in multiplication and growth of trypanosomatid parasites, but comprehensive knowledge is still very limited. In the presen...

journal_title：Biochemical and biophysical research communications

authors： Qureshi R,Jakkula P,Sagurthi SR,Qureshi IA

更新日期：2019-08-27 00:00:00

abstract：:The forced expression of the catalytic subunit of human telomerase, hTERT, produces telomerase activity, allows telomere maintenance, and extends the cellular life span of IMR90 human lung fibroblasts. The mutation D869A abolishes both the catalytic activity of hTERT and its ability to extend cellular life span, demon...

journal_title：Biochemical and biophysical research communications

authors： Ouellette MM,Aisner DL,Savre-Train I,Wright WE,Shay JW

更新日期：1999-01-27 00:00:00

abstract：:We have previously shown that C-CAM1 cell adhesion molecule can suppress the growth of prostate cancer cells in vivo. In this study, we determined the minimal domain of C-CAM1 that is required for its tumor-suppressive activity. DU145 prostate cancer cells were infected with recombinant adenoviruses containing various...

journal_title：Biochemical and biophysical research communications

authors： Estrera VT,Luo W,Phan D,Earley K,Hixson DC,Lin SH

更新日期：1999-10-05 00:00:00

abstract：:A more precise description of the binding of N-(phosphonacetyl)-L- aspartate to the catalytic chains of aspartate carbamoyltransferase clarifies aspects of the specificity of this enzyme toward its substrates, carbamoylphosphate and L-aspartate, and suggests a catalytic role for His-134. ...

journal_title：Biochemical and biophysical research communications

authors： Volź KW,Krause KL,Lipscomb WN

更新日期：1986-04-29 00:00:00

abstract：:Patient-derived xenografts (PDXs) are widely recognised as a more physiologically relevant preclinical model than standard cell lines, but are expensive and low throughput, have low engraftment rate and take a long time to develop. Our newly developed conditional reprogramming (CR) technology addresses many PDX drawba...

journal_title：Biochemical and biophysical research communications

authors： Mondal AM,Ma AH,Li G,Krawczyk E,Yuan R,Lu J,Schlegel R,Stamatakis L,Kowalczyk KJ,Philips GK,Pan CX,Liu X

更新日期：2019-09-10 00:00:00

abstract：:Proteolytic cleavage at specific sites is a key event that modulates protein functions in biological processes. These cleavage sites are identified through mass spectrometry-based peptidomics of overlapping peptide sequences. Here, we assessed to what extent a recent capillary electrophoresis (CE) system interfaced wi...

journal_title：Biochemical and biophysical research communications

authors： Tsuchiya T,Nakayama A,Kawamura T,Sasaki K

更新日期：2020-12-17 00:00:00

abstract：:Insulin inhibits hepatic very low density lipoprotein (VLDL) apo B secretion in rats. Current studies test whether the insulin effect is LDL receptor-mediated by examining the effect of insulin on VLDL apo B secretion in hepatocytes derived from Ldlr-/- and control mice. Primary hepatocytes were incubated overnight wi...

journal_title：Biochemical and biophysical research communications

authors： Chirieac DV,Cianci J,Collins HL,Sparks JD,Sparks CE

更新日期：2002-09-13 00:00:00

abstract：:p70 S6 kinase (p70 S6k) is important in regulating a variety of cellular functions including mRNA translation and cell cycle progression and is activated by mitogens and hormones. Unexpectedly, we have found that, in adult rat cardiomyocytes, arsenite, which generally induces stress responses, markedly and rapidly act...

journal_title：Biochemical and biophysical research communications

authors： Wang X,Proud CG

更新日期：1997-09-08 00:00:00

abstract：:Heme oxygenase cleaves heme to form biliverdin, carbon monoxide (CO), and iron, and consists of two structurally related isozymes, HO-1 and HO-2. HO-2 is also known as a potential oxygen sensor. Here we show that the relative CO content in arterial blood, which reflects the total amount of endogenous heme degradation,...

journal_title：Biochemical and biophysical research communications

authors： Han F,Takeda K,Yokoyama S,Ueda H,Shinozawa Y,Furuyama K,Shibahara S

更新日期：2005-12-09 00:00:00

abstract：:One hypothesis for the etiology of Parkinson's disease (PD) is that the formation of proteinaceous inclusion, which is mainly composed of alpha-synuclein, may contribute to the selective loss of dopaminergic neurons. To further explore the role of alpha-synuclein in neurodegeneration of PD, we examined the possible ef...

journal_title：Biochemical and biophysical research communications

authors： Yu F,Xu H,Zhuo M,Sun L,Dong A,Liu X

更新日期：2005-05-27 00:00:00

abstract：:Autophagy is an evolutionary conserved intracellular process whereby cells break down long-lived proteins and organelles. Accumulating evidences suggest increasing physiological significance of autophagy in pathogenesis of infectious diseases. Anthrax lethal toxin (LT) exerts its influence on numerous cells and herein...

journal_title：Biochemical and biophysical research communications

authors： Tan YK,Kusuma CM,St John LJ,Vu HA,Alibek K,Wu A

更新日期：2009-02-06 00:00:00

abstract：:We used a lactose-substituted polystyrene, poly-N-p-vinylbenzyl-D-lactonamide (PVLA), as a substratum for adult rat hepatocytes in primary culture. Spherical-shaped hepatocytes attached on PVLA substratum formed stable multilayer aggregates anchored on substratum through the stimulation of epidermal growth factor (EGF...

journal_title：Biochemical and biophysical research communications

authors： Tobe S,Takei Y,Kobayashi K,Akaike T

更新日期：1992-04-15 00:00:00

abstract：:The effect of silkworm hemolymph on baculovirus-induced insect cell apoptosis was investigated. The addition of silkworm hemolymph into the culture medium either before or during the baculovirus infection increased the host cell longevity; however, its addition after the infection was less effective. This can be expla...

journal_title：Biochemical and biophysical research communications

authors： Rhee WJ,Park TH

更新日期：2000-04-29 00:00:00

abstract：:The retinoic acid related orphan receptor RORalpha activates transcription of genes that play an important role in cerebellar development, the protection against age-related degenerative processes, the regulation of inflammatory responses, and is one of the pivotal participants that control the circadian rhythmicity i...

journal_title：Biochemical and biophysical research communications

authors： Lechtken A,Hörnig M,Werz O,Corvey N,Zündorf I,Dingermann T,Brandes R,Steinhilber D

更新日期：2007-07-06 00:00:00

abstract：:The diagnostic significance of phosphoglycerate mutase (PGM) B-type isozyme activity capable of being inducible under hypoxia at the gene level as a serum marker for cerebral stroke was investigated. The normal level (mean +/- 2 SD) in human serum was determined to be 38 +/- 18 units/L. Within 2 h after the onset of c...

journal_title：Biochemical and biophysical research communications

authors： Hayashi T,Matuo Y

更新日期：2001-10-05 00:00:00

abstract：:The characterization of a 20 kilodalton (20 kD) variant of rat growth hormone is reported. The 20 kD variant from rat pituitary gland extracts was identified on Western immunoblots of polyacrylamide gels. It was also shown that pituitary tissue maintained in culture secretes the 20 kD form. A rat growth hormone cDNA f...

journal_title：Biochemical and biophysical research communications

authors： Howland DS,Farrington MA,Taylor WD,Hymer WC

更新日期：1987-09-15 00:00:00

abstract：:SPAK and OSR1 are two protein kinases that play important roles in regulating the function of numerous ion co-transporters. They are activated by two distinct mechanisms that involve initial phosphorylation at their T-loops by WNK kinases and subsequent binding to a scaffolding protein termed MO25. To understand this ...

journal_title：Biochemical and biophysical research communications

authors： Mehellou Y,Alamri MA,Dhiani BA,Kadri H

更新日期：2018-09-10 00:00:00

abstract：:Muscarinic receptor extracted from porcine atria in digitonin-cholate copurified with Galpha(o), Galpha(i1-3), and caveolins. The presence of complexes was confirmed by coimmunoprecipitation of the receptor, alpha-subunits, and caveolins in various combinations. Homooligomers of alpha(i2) were detected on Western blot...

journal_title：Biochemical and biophysical research communications

authors： Ma AW,Pawagi AB,Wells JW

更新日期：2008-09-12 00:00:00

abstract：:In order to identify the mechanism by which cyclic AMP stimulates expression of the human renin gene (REN), the effect of forskolin was tested in transient expression analyses of REN 5'-flanking DNA-chloramphenicol acetyltransferase (CAT) reporter gene constructs in secondary cultures of human chorio-decidual cells, a...

journal_title：Biochemical and biophysical research communications

authors： Smith DL,Morris BJ,Do YS,Law RE,Shaw KJ,Hseuh WA

更新日期：1994-04-15 00:00:00

abstract：UNLABELLED:The L-arginine-nitric oxide (NO) pathway was investigated in human internal mammary artery (HIMA) in vitro. HIMA rings were mounted in organ bath, and then incubated in Krebs buffer for 1 to 8 hrs, relaxing agents were tested. Under these conditions, L-arginine (0.1 microM - 1 mM) elicited only minor relaxat...

journal_title：Biochemical and biophysical research communications

authors： Qi X,Chen D,Nottin R,Mace L,Herve P,Weiss M

更新日期：1993-08-31 00:00:00

abstract：:Crystallographic studies have shown independent binding sites for sugar and peptide ligands of concanavalin A, although they were considered functional mimics based on biochemical experiments. The topological correlation of 12-residue peptide with different carbohydrate ligands of concanavalin A showed similarity betw...

journal_title：Biochemical and biophysical research communications

authors： Jain D,Kaur KJ,Goel M,Salunke DM

更新日期：2000-06-16 00:00:00