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Telomerase activity does not always imply telomere maintenance.

Abstract：

:The forced expression of the catalytic subunit of human telomerase, hTERT, produces telomerase activity, allows telomere maintenance, and extends the cellular life span of IMR90 human lung fibroblasts. The mutation D869A abolishes both the catalytic activity of hTERT and its ability to extend cellular life span, demonstrating that the immortalizing capabilities of the enzyme are dependent on active catalysis. A second mutant of hTERT was examined that contains three copies of an HA epitope inserted at the C-terminus. This mutant produced telomerase activity in fibroblasts that was virtually indistinguishable from that of wild type telomerase when assayed in vitro. However, the forced expression of this mutant failed to maintain telomeres or extend cellular life span. Our results show that the catalytic activity of hTERT is required for cellular immortalization but that the presence of active telomerase does not necessarily imply telomere maintenance and immortality.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Ouellette MM,Aisner DL,Savre-Train I,Wright WE,Shay JW

doi

10.1006/bbrc.1998.0114

subject

Has Abstract

pub_date

1999-01-27 00:00:00

pages

795-803

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(98)90114-0

journal_volume

254

pub_type

杂志文章

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