当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Expression of inducible nitric oxide synthase mRNA in rat digestive tissues after endotoxin and its role in intestinal mucosal injury.

Expression of inducible nitric oxide synthase mRNA in rat digestive tissues after endotoxin and its role in intestinal mucosal injury.

Abstract：

:Nitric oxide (NO) production is increased in the intestine and may contribute to intestinal injury in sepsis. However, the tissue expression of inducible NO synthase (iNOS) mRNA throughout the digestive tract and its relation with the mucosal damage after endotoxin challenge remain unknown. We therefore measured tissue expression of mRNA encoding iNOS by Northern blot analysis and reverse transcription PCR. The iNOS mRNA was detectable at 1 h, peaked at 4 h, and remained faint at 24 h after endotoxin injection in esophagus, duodenum, jejunum, ileum, and colon, but not in the stomach. Pre-treatment with dexamethasone attenuated the rise of iNOS mRNA. Both dexamethasone and NOS inhibitor, L-NAME, ameliorated the endotoxin-induced increase in intestinal mucosal permeability. Our results indicate that there is tissue-specific expression of iNOS mRNA in the digestive tract. The manipulations that decrease NO production may have therapeutic potential in preserving intestinal mucosal integrity in sepsis.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Chen K,Inoue M,Okada A

doi

10.1006/bbrc.1996.1087

subject

Has Abstract

pub_date

1996-07-25 00:00:00

pages

703-8

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(96)91087-6

journal_volume

224

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Full active baculovirus-expressed human monocyte chemoattractant protein 1 with the intact N-terminus.

    abstract：:The Sf-9 insect cells infected with a recombinant baculovirus integrated with a cDNA encoding human monocyte chemoattractant protein-1 (MCP-1) produced three different MCP-1s, which were isolated by reverse-phase HPLC as 13kDa, 14kDa, and 15kDa bands on SDS-PAGE. The heterogeneity between these MCP-1s was ascribed to ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1135

    authors： Ishii K,Yamagami S,Tanaka H,Motoki M,Suwa Y,Endo N

    更新日期：1995-01-26 00:00:00

  • Position of cyclization in cyclic ADP-ribose.

    abstract：:Cyclic adenosine diphosphoribose (cADPR) is a putative second messenger of calcium homeostasis synthesized from NAD by cleavage of the nicotinamide-ribose bond and cyclization of the ribose to the adenine ring. In this study, the ultraviolet absorption spectra of cADPR have been studied as a function of pH and compare...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1941

    authors： Kim H,Jacobson EL,Jacobson MK

    更新日期：1993-08-16 00:00:00

  • Differential regulation of cyclin-dependent kinase inhibitors in neuroblastoma cells.

    abstract：:Gastrin-releasing peptide (GRP) and its receptor (GRP-R) are highly expressed in undifferentiated neuroblastoma, and they play critical roles in oncogenesis. We previously reported that GRP activates the PI3K/AKT signaling pathway to promote DNA synthesis and cell cycle progression in neuroblastoma cells. Conversely, ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.04.023

    authors： Qiao L,Paul P,Lee S,Qiao J,Wang Y,Chung DH

    更新日期：2013-05-31 00:00:00

  • Proteinase B is, indeed, not required for chitin synthetase 1 function in Saccharomyces cerevisiae.

    abstract：:Previous genetic evidence led to the conclusion that proteinase B of yeast was not involved in the function of chitin synthetase 1 (Chs1), based on the demonstration of normal septum formation, cell division and chitin deposition in mutants devoid of the proteinase (Zubenko, G.S., Mitchell, A.P., and Jones, E.W. (1979...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)90506-3

    authors： Silverman SJ,Shaw JA,Cabib E

    更新日期：1991-01-15 00:00:00

  • NLRP3/ASC/Caspase-1 axis and serine protease activity are involved in neutrophil IL-1β processing during Streptococcus pneumoniae infection.

    abstract：:Streptococcus pneumoniae is a pathogenic bacterium that can cause severe invasive diseases, such as pneumonia, otitis media and meningitis. The pro-inflammatory cytokine, IL-1β, has been reported to play important role in host defense against S. pneumoniae. The mechanism of IL-1β maturation and secretion in macrophage...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.04.004

    authors： Zhang T,Du H,Feng S,Wu R,Chen T,Jiang J,Peng Y,Ye C,Fang R

    更新日期：2019-06-04 00:00:00

  • WITHDRAWN: Ambra1 modulates starvation-induced autophagy through AMPK signaling pathway in cardiomyocytes.

    abstract：:This article has been withdrawn at the request of the author(s). The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.05.007

    authors： Shi C,Wu J,Fu M,Zhang B,Wang J,Yang X,Chi Y

    更新日期：2014-05-10 00:00:00

  • Articular cartilage vesicles contain RNA.

    abstract：:Small membrane-bound extracellular organelles known as articular cartilage matrix vesicles (ACVs) participate in pathologic mineralization in osteoarthritic articular cartilage. ACVs are also present in normal cartilage, although they have no known functions other than mineralization. Recently, RNA was identified in e...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.08.038

    authors： Mitton E,Gohr CM,McNally MT,Rosenthal AK

    更新日期：2009-10-23 00:00:00

  • An in vitro evaluation of the effects of homocysteine thiolactone on key steps of angiogenesis and tumor invasion.

    abstract：:Homocysteine thiolactone is a highly reactive homocysteine derivative that can react easily with proteins. Protein homocysteinylation has been suggested as a possible mechanism underlying the pathological consequences of impaired homocysteine metabolism. Homocysteine inhibits key steps of angiogenesis and tumor invasi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.10.044

    authors： Martínez-Poveda B,Chavarría T,Sánchez-Jiménez F,Quesada AR,Medina MA

    更新日期：2003-11-21 00:00:00

  • Variable allelic expression of imprinted genes in human pluripotent stem cells during differentiation into specialized cell types in vitro.

    abstract：:Genomic imprinting is an epigenetic phenomenon by which a subset of genes is asymmetrically expressed in a parent-of-origin manner. However, little is known regarding the epigenetic behaviors of imprinted genes during human development. Here, we show dynamic epigenetic changes in imprinted genes in hESCs during in vit...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.02.141

    authors： Park SW,Kim J,Park JL,Ko JY,Im I,Do HS,Kim H,Tran NT,Lee SH,Kim YS,Cho YS,Lee DR,Han YM

    更新日期：2014-04-04 00:00:00

  • Rapid grouping of HIV-1 infection in subtypes A to E by V3 peptide serotyping and its relation to sequence analysis.

    abstract：:We developed a typing assay for HIV-1 using subtype specific peptides corresponding to the five major subtypes of HIV-1 (A to E). In eight patients serologically subtyped as A (n = 1), B (n = 3), C (n = 3) and E (n = 1) phyllogenetic analysis of sequenced V3 domain DNA completely correlated to the peptide serotyping. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2858

    authors： Sherefa K,Sönnerborg A,Steinbergs J,Sällberg M

    更新日期：1994-12-30 00:00:00

  • Bone morphogenetic protein.

    abstract：:Bone morphogenetic proteins (BMPs) are multi-functional growth factors belonging to the transforming growth factor-beta superfamily. It has been demonstrated that BMPs had been involved in the regulation of cell proliferation, survival, differentiation and apoptosis. However, their hallmark ability is that play a pivo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2007.08.045

    authors： Xiao YT,Xiang LX,Shao JZ

    更新日期：2007-10-26 00:00:00

  • Proatherogenic flow conditions initiate endothelial apoptosis via thrombospondin-1 and the integrin-associated protein.

    abstract：:Recently it has been shown that vascular endothelial cells (EC) are completely devoid of apoptosis if cultivated under a steady laminar flow and that apoptosis is induced by turning off the flow. An autocrine loop of thrombospondin-1 (TSP-1) and the alpha(v)beta(3) integrin/integrin-associated protein (IAP) complex ha...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5314

    authors： Freyberg MA,Kaiser D,Graf R,Buttenbender J,Friedl P

    更新日期：2001-08-10 00:00:00

  • The role of active site residues in the oxidant specificity of the Orp1 thiol peroxidase.

    abstract：:In this study we investigated the role of active site residues in the peroxidase activity of Orp1 (GPx3) using three different peroxide substrates. Using a structural homology model of the reduced form of Orp1, we identified Asn126 and Phe127 as evolutionarily conserved residues that line the back of the Orp1 active s...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.10.109

    authors： Takanishi CL,Ma LH,Wood MJ

    更新日期：2010-12-03 00:00:00

  • Modulation of airway epithelial cell ciliary beat frequency by nitric oxide.

    abstract：:NOS activity has been recently described in airway epithelial cells. Because these cells are often ciliated we hypothesized that NO modulates airway ciliary beating. CBF was measured in cultured BBECs using video microscopy. L-NMMA, a NOS inhibitor, caused a 40% decrease in CBF following pre-stimulation with isoproter...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1187

    authors： Jain B,Rubinstein I,Robbins RA,Leise KL,Sisson JH

    更新日期：1993-02-26 00:00:00

  • In-house made nucleofection buffer for efficient and cost effective transfection of RAW 264.7 macrophages.

    abstract：:Electroporation is the most widely employed method of gene transfer into macrophages which are hard to transfect. RAW 264.7 is a widely used cell line for studying macrophage responses. Electroporation of RAW 264.7 cells with commercial reagents although very efficient is expensive necessitating the development of cos...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.04.043

    authors： Alamuru-Yellapragada NP,Kapadia B,Parsa KVL

    更新日期：2017-05-27 00:00:00

  • Conserved GC-boxes, E-box and GATA motif are essential for GATA-4 gene expression in P19CL6 cells.

    abstract：:The promoter of the GATA-4 gene was analyzed in P19CL6 cells. A 124bp segment containing conserved two GC-boxes and E-box was essential for the basal promoter activity, as determined with a transient luciferase reporter gene assay. However, an extended 1312 bp reporter construct but not the 124 bp segment, when ligate...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.08.036

    authors： Ishibashi T,Yokura Y,Ohashi K,Yamamoto H,Maeda M

    更新日期：2011-09-23 00:00:00

  • Annexin II is a novel receptor for Pseudomonas aeruginosa.

    abstract：:Infections with Pseudomonas aeruginosa (P. aeruginosa) are critical in ventilated and poly-traumatized patients. Most important, these bacteria cause frequent and chronic pulmonary infections in patients with cystic fibrosis. Therefore, identification of molecular mechanisms that mediate the infection of mammalian cel...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.12.089

    authors： Kirschnek S,Adams C,Gulbins E

    更新日期：2005-02-18 00:00:00

  • A novel protease inhibitor causes inclusion vacuole reduction and disrupts the intracellular growth of Chlamydia trachomatis.

    abstract：:Chlamydia (C.) trachomatis, characterized by a unique biphasic life cycle, is an obligate intracellular bacterial pathogen which is responsible for the highest number of sexually transmitted bacterial infections globally. However, its pathogenic mechanisms have not been fully elucidated because of its unique developme...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.05.184

    authors： Zhou Y,Lu X,Huang D,Lu Y,Zhang H,Zhang L,Yu P,Wang F,Wang Y

    更新日期：2019-08-13 00:00:00

  • Inhibition of the vacuolar H(+)-pump with bafilomycin A1 does not induce acrosome reaction or activate proacrosin in mouse spermatozoa.

    abstract：:Acrosomal protease activation is regarded as an important event triggered by acrosomal reaction and leading to sperm passage through zona pellucida. Mammalian acrosome has an internal acid pH that probably helps to maintain inactive proenzymes that otherwise could be precociously activated and prevent normal fertiliza...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.10.002

    authors： Codelia VA,Cortes CJ,Moreno RD

    更新日期：2005-12-02 00:00:00

  • Long non-coding RNA HNF1A-AS1 functioned as an oncogene and autophagy promoter in hepatocellular carcinoma through sponging hsa-miR-30b-5p.

    abstract：:Long non-coding RNAs (lncRNAs) had been proved to be pivotal regulators in carcinogenesis. On the basis of competitive endogenous RNAs (ceRNAs) system, lncRNAs significantly expanded their regulating networks. In our research, we aimed to figure out the exact role of lncRNA HNF1A-AS1 in the pathogenesis of hepatocellu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.04.054

    authors： Liu Z,Wei X,Zhang A,Li C,Bai J,Dong J

    更新日期：2016-05-13 00:00:00

  • The reversibility of the glutathionyl-quercetin adduct spreads oxidized quercetin-induced toxicity.

    abstract：:Quercetin is one of the most prominent dietary antioxidants. During its antioxidant activity, quercetin becomes oxidized into its o-quinone/quinone methide QQ. QQ is toxic since it instantaneously reacts with thiols of, e.g., proteins. In cells, QQ will initially form an adduct with glutathione (GSH), giving GSQ. We h...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.10.031

    authors： Boots AW,Balk JM,Bast A,Haenen GR

    更新日期：2005-12-16 00:00:00

  • Physical association of Ras and cathepsins B and L in the conditioned medium of v-Ha-ras-transformed NIH3T3 cells.

    abstract：:Immunoblot analysis of the conditioned medium of v-Ha-ras-transformed NIH3T3 cells showed that Ras protein is secreted in the culture fluid. Because cathepsins L and B whose activities are inhibited by Ras are also secreted, possible association between Ras and cathepsins was investigated by immunoprecipitation and fo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2696

    authors： Hiwasa T,Kominami E

    更新日期：1995-11-22 00:00:00

  • Vaccination with a DNA vaccine encoding CD317-targeting HBs antigen elicits enhanced immunity in mice.

    abstract：BACKGROUND:Conventional hepatitis B virus (HBV) vaccines fail to induce protective antibody titers in 5-10% of immune-competent vaccines. Therefore, safe and effective HBV vaccines are still clinically needed. METHODS:In this study, we developed a plasmid DNA vaccine encoding CD317 single-chain fragment variable (α317...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.09.024

    authors： Zhang G,Li X,Liu L,Li J,Chen Q,Huang S,Li Y,Wan X

    更新日期：2018-10-12 00:00:00

  • Modulation of RAB5A early endosome trafficking in response to KRas mediated macropinocytic fluxes in pancreatic cancer cells.

    abstract：:KRAS is the key mutated gene in pancreatic ductal adenocarcinoma (PDAC). Emerging evidence indicates that KRas modulates endocytic uptake. The present study aimed to explore the fate of early endosomal trafficking under the control of KRas expression in PDAC. Surprisingly, PANC-1 cells lacking KRas exhibited significa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.08.157

    authors： Teske C,Schweitzer C,Palamidessi A,Aust DE,Scita G,Weitz J,Welsch T

    更新日期：2017-11-04 00:00:00

  • Insulin-dependent phosphatidylinositol 3'-kinase activity co-precipitates with insulin receptor in human circulating mononuclear cells.

    abstract：:In order to establish a working cellular model for the study of post-receptor signaling events, insulin-dependent phosphatidylinositol 3' (PtdIns 3')-kinase activity was investigated in circulating mononuclear cells from normal subjects. The p85 alpha regulatory subunit of PtdIns 3'-kinase co-precipitated with activat...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1494

    authors： Renard E,Grigorescu F,Lavabre C,Kahn CR

    更新日期：1995-04-06 00:00:00

  • Adipose tissue-deprived stem cells acquire cementoblast features treated with dental follicle cell conditioned medium containing dentin non-collagenous proteins in vitro.

    abstract：:Adipose tissue-derived stem cells (ADSCs), which are easily harvested and show excellent pluripotency potential, have generated considerable interest in regenerative medicine. In this study, the differentiation of ADSCs was assessed after treatment with dental follicle cell conditioned medium (DFCCM) containing dentin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.05.067

    authors： Wen X,Nie X,Zhang L,Liu L,Deng M

    更新日期：2011-06-10 00:00:00

  • The effect of variable domain orientation and arrangement on the antigen-binding activity of a recombinant human bispecific diabody.

    abstract：:In recent years a variety of recombinant methods have been developed for efficient production of bispecific antibodies (BsAb) in various formats. Bispecific diabody (bDAb), a 55-60 kDa molecule comprising two non-covalently associated cross-over single chain Fv (scFv) polypeptides, represents one of the most promising...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.04.060

    authors： Lu D,Jimenez X,Witte L,Zhu Z

    更新日期：2004-05-28 00:00:00

  • Regulated expression of 5'-deleted mouse GLUT4 minigenes in transgenic mice: effects of exercise training and high-fat diet.

    abstract：:Fourteen kb murine GLUT4 minigene (= -7395 GLUT4) contains DNA sequence that confers tissue specific, exercise-induced up-regulation of the GLUT4 gene in skeletal muscle and high-fat diet induced-down-regulation in white adipose tissue. To identify the DNA sequences required for regulated expression, we generated GLUT...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7516

    authors： Tsunoda N,Cooke DW,Ikemoto S,Maruyama K,Takahashi M,Lane MD,Ezaki O

    更新日期：1997-10-20 00:00:00

  • Crystal structure of the protein kinase domain of yeast AMP-activated protein kinase Snf1.

    abstract：:AMP-activated protein kinase (AMPK) is a master metabolic regulator, and is an important target for drug development against diabetes, obesity, and other diseases. AMPK is a hetero-trimeric enzyme, with a catalytic (alpha) subunit, and two regulatory (beta and gamma) subunits. Here we report the crystal structure at 2...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.09.181

    authors： Rudolph MJ,Amodeo GA,Bai Y,Tong L

    更新日期：2005-12-02 00:00:00

  • Alzheimer's disease brain extract stimulates the survival of cerebral cortical neurons from neonatal rats.

    abstract：:Cell cultures of neonatal rat cerebral cortex in a serum-free medium were used to investigate a lack of neuronotrophic factors in Alzheimer's disease (AD) brain. A few neurons survived in the absence of brain extract. The addition of normal brain extract resulted in a 2.5-fold increase in neuronal survival. AD brain e...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(88)90765-6

    authors： Uchida Y,Ihara Y,Tomonaga M

    更新日期：1988-02-15 00:00:00